Differential effects of central leptin, insulin, or glucose administration during fasting on the hypothalamic-pituitary-thyroid axis and feeding-related neurons in the arcuate nucleus

Csaba Fekete, Praful S. Singru, Edith Sanchez, Sumit Sarkar, Marcelo A. Christoffolete, Rogerio S. Riberio, William M. Rand, Charles H. Emerson, Antonio C. Bianco, Ronald M. Lechan

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The reductions in circulating levels of leptin, insulin, and glucose with fasting serve as important homeostasis signals to neurons of the hypothalamic arcuate nucleus that synthesize neuropeptide Y (NPY)/agouti-related protein (AGRP) and α-MSH/cocaine and amphetamine-regulated transcript. Because the central administration of leptin is capable of preventing the inhibitory effects of fasting on TRH mRNA in hypophysiotropic neurons primarily through effects on the arcuate nucleus, we determined whether the continuous administration of 30 mU/d insulin or 648 μg/d glucose into the cerebrospinal fluid by osmotic minipump might also have similar effects on the hypothalamic-pituitary-thyroid axis. As anticipated, the intracerebroventricular infusion of leptin reduced fasting-induced elevations in NPY and AGRP mRNA and increased proopiomelanocortin and cocaine and amphetamine-regulated transcript mRNA in the arcuate nucleus. In addition, leptin prevented fasting-induced reduction in pro-TRH mRNA levels in the paraventricular nucleus and in circulating thyroid hormone levels. In contrast, whereas insulin increased proopiomelanocortin mRNA and both insulin and glucose reduced NPY mRNA in arcuate nucleus neurons, neither prevented the fasting-induced suppression in hypophysiotropic TRH mRNA or circulating thyroid hormone levels. We conclude that insulin and glucose only partially replicate the central effects of leptin and may not be essential components of the hypothalamic-pituitary-thyroid regulatory system during fasting.

Original languageEnglish
Pages (from-to)520-529
Number of pages10
JournalEndocrinology
Volume147
Issue number1
DOIs
StatePublished - Jan 1 2006

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Arcuate Nucleus of Hypothalamus
Leptin
Fasting
Thyroid Gland
Insulin
Neurons
Glucose
Messenger RNA
Neuropeptide Y
Agouti-Related Protein
Pro-Opiomelanocortin
Amphetamine
Thyroid Hormones
Cocaine
Intraventricular Infusions
Melanocyte-Stimulating Hormones
Paraventricular Hypothalamic Nucleus
Cerebrospinal Fluid
Homeostasis

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Differential effects of central leptin, insulin, or glucose administration during fasting on the hypothalamic-pituitary-thyroid axis and feeding-related neurons in the arcuate nucleus. / Fekete, Csaba; Singru, Praful S.; Sanchez, Edith; Sarkar, Sumit; Christoffolete, Marcelo A.; Riberio, Rogerio S.; Rand, William M.; Emerson, Charles H.; Bianco, Antonio C.; Lechan, Ronald M.

In: Endocrinology, Vol. 147, No. 1, 01.01.2006, p. 520-529.

Research output: Contribution to journalArticle

Fekete, C, Singru, PS, Sanchez, E, Sarkar, S, Christoffolete, MA, Riberio, RS, Rand, WM, Emerson, CH, Bianco, AC & Lechan, RM 2006, 'Differential effects of central leptin, insulin, or glucose administration during fasting on the hypothalamic-pituitary-thyroid axis and feeding-related neurons in the arcuate nucleus', Endocrinology, vol. 147, no. 1, pp. 520-529. https://doi.org/10.1210/en.2005-0956
Fekete, Csaba ; Singru, Praful S. ; Sanchez, Edith ; Sarkar, Sumit ; Christoffolete, Marcelo A. ; Riberio, Rogerio S. ; Rand, William M. ; Emerson, Charles H. ; Bianco, Antonio C. ; Lechan, Ronald M. / Differential effects of central leptin, insulin, or glucose administration during fasting on the hypothalamic-pituitary-thyroid axis and feeding-related neurons in the arcuate nucleus. In: Endocrinology. 2006 ; Vol. 147, No. 1. pp. 520-529.
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