Abstract
This study was designed to determine whether the various classes of Ca2+ channel blockers have differential protective effects on isolated adult rat ventricular myocytes exposed to high K+ under anoxic (100% N2) conditions. Calcium-tolerant myocytes were incubated under control (4 mm K+) aerobic conditions and then subjected to high K+ (75 mm) and N2. The cells were assessed by morphological criteria (i.e. absence of blebbing, granulation etc.), maintenance of ATP levels, exclusion of trypan blue, and the presence or absence of spontaneous contractile activity. Under control conditions, the cells were quiescent and declined at a rate of ≈10%/h. In the absence of O2, the rate of cell decline was significantly faster. Verapamil, diltiazem and the dihydropyridines had no significant effects on cell decline under these conditions. Cells exposed to 75 mm K0+ exhibited contractile activity and accelerated rate of decline under anoxic conditions; these effects were independent of lowering Na0+ to 75 mm. Cells in high K0+ and N2 were significantly protected (i.e. contractile activity and rate of decline were decreased) by verapamil, less so by diltiazem, and not at all by the dihydropyridines. The uptake of 45Ca2+ into cells in high K0+ was not significantly altered by verapamil or diltiazem. Caffeine induced the immediate cessation of contractile activity of cells incubated in high K0+, but did not affect the accelerated rate of cell decline under anoxic conditions. Verapamil and diltiazem still conferred significant protection in this non-beating cell preparation. Neither verapamil nor diltiazem had any effect on the oscillation frequency of skinned heart cells. The data indicate that, based on their sites and mechanisms of action, the various classes of Ca2+ antagonists can differentially affect cells under pathophysiological conditions. Verapamil and diltiazem may act at the sarcolemmal membrane of depolarized cells at sites independent of the Na+/Ca2+ exchange carrier and the fast inactivating Ca2+ channel.
| Original language | English |
|---|---|
| Pages (from-to) | 1129-1137 |
| Number of pages | 9 |
| Journal | Journal of Molecular and Cellular Cardiology |
| Volume | 17 |
| Issue number | 12 |
| DOIs | |
| State | Published - Jan 1 1985 |
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Keywords
- Calcium antagonists
- Cardiac myocytes
- Dihydropyridines
- Diltiazem
- Verapamil
ASJC Scopus subject areas
- Molecular Biology
- Cardiology and Cardiovascular Medicine
Cite this
Differential effects of calcium antagonists on viability of adult rat ventricular myocytes. / Miller, Linda S.; Barrett, John; Cameron, John S.; Bassett, Arthur L.
In: Journal of Molecular and Cellular Cardiology, Vol. 17, No. 12, 01.01.1985, p. 1129-1137.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential effects of calcium antagonists on viability of adult rat ventricular myocytes
AU - Miller, Linda S.
AU - Barrett, John
AU - Cameron, John S.
AU - Bassett, Arthur L.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - This study was designed to determine whether the various classes of Ca2+ channel blockers have differential protective effects on isolated adult rat ventricular myocytes exposed to high K+ under anoxic (100% N2) conditions. Calcium-tolerant myocytes were incubated under control (4 mm K+) aerobic conditions and then subjected to high K+ (75 mm) and N2. The cells were assessed by morphological criteria (i.e. absence of blebbing, granulation etc.), maintenance of ATP levels, exclusion of trypan blue, and the presence or absence of spontaneous contractile activity. Under control conditions, the cells were quiescent and declined at a rate of ≈10%/h. In the absence of O2, the rate of cell decline was significantly faster. Verapamil, diltiazem and the dihydropyridines had no significant effects on cell decline under these conditions. Cells exposed to 75 mm K0+ exhibited contractile activity and accelerated rate of decline under anoxic conditions; these effects were independent of lowering Na0+ to 75 mm. Cells in high K0+ and N2 were significantly protected (i.e. contractile activity and rate of decline were decreased) by verapamil, less so by diltiazem, and not at all by the dihydropyridines. The uptake of 45Ca2+ into cells in high K0+ was not significantly altered by verapamil or diltiazem. Caffeine induced the immediate cessation of contractile activity of cells incubated in high K0+, but did not affect the accelerated rate of cell decline under anoxic conditions. Verapamil and diltiazem still conferred significant protection in this non-beating cell preparation. Neither verapamil nor diltiazem had any effect on the oscillation frequency of skinned heart cells. The data indicate that, based on their sites and mechanisms of action, the various classes of Ca2+ antagonists can differentially affect cells under pathophysiological conditions. Verapamil and diltiazem may act at the sarcolemmal membrane of depolarized cells at sites independent of the Na+/Ca2+ exchange carrier and the fast inactivating Ca2+ channel.
AB - This study was designed to determine whether the various classes of Ca2+ channel blockers have differential protective effects on isolated adult rat ventricular myocytes exposed to high K+ under anoxic (100% N2) conditions. Calcium-tolerant myocytes were incubated under control (4 mm K+) aerobic conditions and then subjected to high K+ (75 mm) and N2. The cells were assessed by morphological criteria (i.e. absence of blebbing, granulation etc.), maintenance of ATP levels, exclusion of trypan blue, and the presence or absence of spontaneous contractile activity. Under control conditions, the cells were quiescent and declined at a rate of ≈10%/h. In the absence of O2, the rate of cell decline was significantly faster. Verapamil, diltiazem and the dihydropyridines had no significant effects on cell decline under these conditions. Cells exposed to 75 mm K0+ exhibited contractile activity and accelerated rate of decline under anoxic conditions; these effects were independent of lowering Na0+ to 75 mm. Cells in high K0+ and N2 were significantly protected (i.e. contractile activity and rate of decline were decreased) by verapamil, less so by diltiazem, and not at all by the dihydropyridines. The uptake of 45Ca2+ into cells in high K0+ was not significantly altered by verapamil or diltiazem. Caffeine induced the immediate cessation of contractile activity of cells incubated in high K0+, but did not affect the accelerated rate of cell decline under anoxic conditions. Verapamil and diltiazem still conferred significant protection in this non-beating cell preparation. Neither verapamil nor diltiazem had any effect on the oscillation frequency of skinned heart cells. The data indicate that, based on their sites and mechanisms of action, the various classes of Ca2+ antagonists can differentially affect cells under pathophysiological conditions. Verapamil and diltiazem may act at the sarcolemmal membrane of depolarized cells at sites independent of the Na+/Ca2+ exchange carrier and the fast inactivating Ca2+ channel.
KW - Calcium antagonists
KW - Cardiac myocytes
KW - Dihydropyridines
KW - Diltiazem
KW - Verapamil
UR - http://www.scopus.com/inward/record.url?scp=0022359680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022359680&partnerID=8YFLogxK
U2 - 10.1016/S0022-2828(85)80109-7
DO - 10.1016/S0022-2828(85)80109-7
M3 - Article
VL - 17
SP - 1129
EP - 1137
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
SN - 0022-2828
IS - 12
ER -