Differential effects in vivo of thyroid hormone on the expression of surfactant phospholipid, surfactant protein mRNA and antioxidant enzyme mRNA in fetal rat lung

Sujatha M. Ramadurai, Heber C. Nielsen, Youwei Chen, Dimitrios Hatzis, Ilene Sosenko

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Antenatal administration of triiodo-L-thyronine (T3) to late gestation rates resulted in decreased lung antioxidant enzyme (AOE) activity but increased surfactant phospholipids. In fetal rat lung explant cultures, T3 decreased the expression of surfactant proteins (SP) A and B. There have been no reported studies of the simultaneous in vivo developmental influence of T3 on both pulmonary AOE and SP gene expression. We hypothesized that antenatal T3 treatment would cause differential regulation of surfactant phospholipid, SP, and AOE genes in the late question fetal rat. Timed pregnant rats received intramuscular injections of either T3 (7 mg/kg) or placebo on days 19 and 20 of gestation and fetuses were delivered on day 21. Fetal lung SP-A, SP-B, SP-C, and AOE mRNA levels were studied by Northern analysis. AOE mRNA levels were further quantitated by solution hybridization. Total lung phospholipids (TPL and disaturated phosphatidylcholine (DSPC) content were quantitated by a phosphorus assay. T3 significantly increased TPL and DSPC content, and significantly decreased the expression of SP-A, SP- C, CuZnSOD, and catalase genes. Because of a crucial interplay of these factors for normal lung function at the time of birth, the molecular mechanisms by which these apparently opposing changes are accomplished warrant further investigation.

Original languageEnglish
Pages (from-to)641-657
Number of pages17
JournalExperimental Lung Research
Volume24
Issue number5
StatePublished - Oct 13 1998

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Thyroid Hormones
Surface-Active Agents
Rats
Phospholipids
Antioxidants
Lung
Messenger RNA
Pulmonary Surfactant-Associated Protein A
Enzymes
Proteins
Protein C
Pulmonary Surfactant-Associated Proteins
Genes
Thyronines
Fetal Proteins
Pregnancy
Intramuscular Injections
Enzyme activity
Gene expression
Catalase

Keywords

  • Antioxidant enzymes (ADE)
  • Iri-iodothyronine (T)
  • Surfactant protein genes (SP)

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Differential effects in vivo of thyroid hormone on the expression of surfactant phospholipid, surfactant protein mRNA and antioxidant enzyme mRNA in fetal rat lung. / Ramadurai, Sujatha M.; Nielsen, Heber C.; Chen, Youwei; Hatzis, Dimitrios; Sosenko, Ilene.

In: Experimental Lung Research, Vol. 24, No. 5, 13.10.1998, p. 641-657.

Research output: Contribution to journalArticle

Ramadurai, Sujatha M. ; Nielsen, Heber C. ; Chen, Youwei ; Hatzis, Dimitrios ; Sosenko, Ilene. / Differential effects in vivo of thyroid hormone on the expression of surfactant phospholipid, surfactant protein mRNA and antioxidant enzyme mRNA in fetal rat lung. In: Experimental Lung Research. 1998 ; Vol. 24, No. 5. pp. 641-657.
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