Differential Effect of Ionizing Radiation on Transcription in Repair-deficient and Repair-proficient Mice

George P. Munson, Gayle E. Woloschak

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Experiments were designed to examine in vivo changes in total transcription and in the expression of the c-fos gene following whole-body exposure of mice to JANUS fission-spectrum neutrons. Radiation repair-deficient (wst/wst) and -proficient (wstfc C57BL/6 x C3H FO mice were exposed to JANUS fission-spectrum neutrons calibrated to deliver a gut dose of 50 cGy. Animals were sacrificed <10 or at 60 min postirradiation, and gut tissues were removed for study. Our results revealed that, in repair-proficient mice, an immediate depression (relative to untreated controls) in total transcription was evident that continued through 1 h postirradiation. Conversely, radiation-sensitive wst/wst mice displayed doubled transcription levels postirradiation. Expression of c-fos was consistently depressed following radiation exposure in control and wst/wst mice. However, the depression of c-fos mRNA was delayed in wst/wst mice relative to controls. These results demonstrate abnormal regulation of transcription and of c-fos mRNA accumulation in repair-deficient wasted mice following exposure to ionizing radiation. In addition, this work documents rapid total transcriptional depression in normal mice following radiation exposure.

Original languageEnglish (US)
Pages (from-to)5045-5048
Number of pages4
JournalCancer Research
Volume50
Issue number16
StatePublished - Aug 15 1990
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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