Differential effect of cyclooxygenase inhibitors on absorptive hyperemia

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4 Scopus citations

Abstract

To test whether the hydrolytic products of digestion could stimulate vasoactive prostaglandin synthesis in the intestine, the muscularis and mucosa of the rat jejunum were suffused with a biocarbonate-buffered Ringer vehicle containing cyclooxygenase inhibitors (meclofenamate or indomethacin; 3 x 10-5 M). To evoke blood flow changes, either glucose (56 mM), oleic acid (20 or 40 mM), or sodium arachidonate was added to the mucosal vehicle. Bile salt (taurocholic acid, 10 mM) was added to emulsify oleic acid. Blood flow was calculated (BF(c)) in submucosal arterioles by use of video microscopy. Neither bile salt nor cyclooxygenase inhibitors altered resting BF(c). Neither oleic acid concentration nor solution osmolality altered the magnitude of absorptive hyperemia. After 10 min of glucose, BF(c) increased 36 ± 6% with vehicle (n = 16) and 39 ± 8% with meclofenamate (n = 10). After 10 min of oleic acid, BF(c) increased 21 ± 5% with vehicle (n = 17) and 34 ± 6% with inhibitors (n = 17). In animals exposed twice to the same concentration of oleic acid, the paired difference between the vehicle and inhibitors (19 ± 7%; n = 9) was significant. Arachidonate alone produced no dose-related (0.06-1.9 mM) effect on BF(c) (n = 41), but arachidonate (0.3 mM) combined with cyclooxygenase inhibitors (6 x 10-5 M) produced a significant BF(c) increase of 35 ± 7% (n = 6). These observations suggest that the absorption of oleic acid, but not glucose, stimulates the synthesis of a vasoactive metabolite of arachidonate. The mechanisms for the differential effect of clyclooxygenase inhibitors is unknown but could involve nonprostaglandin metabolites or arachidonate, such as lipoxygenase of cytochrome P-450 products.

Original languageEnglish (US)
Pages (from-to)H755-H762
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume18
Issue number4
StatePublished - Jan 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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