Abstract
CD1d-restricted type I NKT cells provide help for specific antibody production. B cells, which have captured and presented a T-dependent, antigen-derived peptide on MHC class II and CD1d-binding glycolipid α-GC on CD1d, respectively, activate Th and NKT cells to elicit B cell help. However, the role of the DC CD1d in humoral immunity remains unknown. We therefore constructed mixed bone marrow chimeras containing CD1d-ex-pressing, DTR-transgenic DCs and CD1d+ or CD1d nontransgenic DCs. Following DT-mediated DC ablation and immunization, we observed that the primary and secondary antibody responses were equivalent in the presence of CD1d+ and CD1d DCs. In contrast, a total ablation of DCs delayed the primary antibody response. Further experiments revealed that depletion of CD1d+ DCs blocked in vivo expansion of antigen-specific cytotoxic (CD8+) T lymphocytes. These results provide a clear demonstration that although CD1d expression on DCs is essential for NKT-enhanced CD8+ T cell expansion, it is dispensable for specific antibody production.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 783-790 |
| Number of pages | 8 |
| Journal | Journal of Leukocyte Biology |
| Volume | 91 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 1 2012 |
| Externally published | Yes |
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Keywords
- α-Galactosylceramide
- Adaptive Immunity
- Antibody
- Antigen Presentation
ASJC Scopus subject areas
- Cell Biology
- Immunology
Cite this
Differential contribution of dendritic cell CD1d to NKT cell-enhanced humoral immunity and CD8+ T cell activation. / Joshi, Sunil; Lang, Gillian A.; Devera, T. Scott; Johnson, Amy M.; Kovats, Susan; Lang, Mark L.
In: Journal of Leukocyte Biology, Vol. 91, No. 5, 01.05.2012, p. 783-790.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential contribution of dendritic cell CD1d to NKT cell-enhanced humoral immunity and CD8+ T cell activation
AU - Joshi, Sunil
AU - Lang, Gillian A.
AU - Devera, T. Scott
AU - Johnson, Amy M.
AU - Kovats, Susan
AU - Lang, Mark L.
PY - 2012/5/1
Y1 - 2012/5/1
N2 - CD1d-restricted type I NKT cells provide help for specific antibody production. B cells, which have captured and presented a T-dependent, antigen-derived peptide on MHC class II and CD1d-binding glycolipid α-GC on CD1d, respectively, activate Th and NKT cells to elicit B cell help. However, the role of the DC CD1d in humoral immunity remains unknown. We therefore constructed mixed bone marrow chimeras containing CD1d-ex-pressing, DTR-transgenic DCs and CD1d+ or CD1d nontransgenic DCs. Following DT-mediated DC ablation and immunization, we observed that the primary and secondary antibody responses were equivalent in the presence of CD1d+ and CD1d DCs. In contrast, a total ablation of DCs delayed the primary antibody response. Further experiments revealed that depletion of CD1d+ DCs blocked in vivo expansion of antigen-specific cytotoxic (CD8+) T lymphocytes. These results provide a clear demonstration that although CD1d expression on DCs is essential for NKT-enhanced CD8+ T cell expansion, it is dispensable for specific antibody production.
AB - CD1d-restricted type I NKT cells provide help for specific antibody production. B cells, which have captured and presented a T-dependent, antigen-derived peptide on MHC class II and CD1d-binding glycolipid α-GC on CD1d, respectively, activate Th and NKT cells to elicit B cell help. However, the role of the DC CD1d in humoral immunity remains unknown. We therefore constructed mixed bone marrow chimeras containing CD1d-ex-pressing, DTR-transgenic DCs and CD1d+ or CD1d nontransgenic DCs. Following DT-mediated DC ablation and immunization, we observed that the primary and secondary antibody responses were equivalent in the presence of CD1d+ and CD1d DCs. In contrast, a total ablation of DCs delayed the primary antibody response. Further experiments revealed that depletion of CD1d+ DCs blocked in vivo expansion of antigen-specific cytotoxic (CD8+) T lymphocytes. These results provide a clear demonstration that although CD1d expression on DCs is essential for NKT-enhanced CD8+ T cell expansion, it is dispensable for specific antibody production.
KW - α-Galactosylceramide
KW - Adaptive Immunity
KW - Antibody
KW - Antigen Presentation
UR - http://www.scopus.com/inward/record.url?scp=84860911029&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860911029&partnerID=8YFLogxK
U2 - 10.1189/jlb.1111559
DO - 10.1189/jlb.1111559
M3 - Article
C2 - 22331103
AN - SCOPUS:84860911029
VL - 91
SP - 783
EP - 790
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 5
ER -