Differential contribution of dendritic cell CD1d to NKT cell-enhanced humoral immunity and CD8+ T cell activation

Sunil Joshi, Gillian A. Lang, T. Scott Devera, Amy M. Johnson, Susan Kovats, Mark L. Lang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

CD1d-restricted type I NKT cells provide help for specific antibody production. B cells, which have captured and presented a T-dependent, antigen-derived peptide on MHC class II and CD1d-binding glycolipid α-GC on CD1d, respectively, activate Th and NKT cells to elicit B cell help. However, the role of the DC CD1d in humoral immunity remains unknown. We therefore constructed mixed bone marrow chimeras containing CD1d-ex-pressing, DTR-transgenic DCs and CD1d+ or CD1d nontransgenic DCs. Following DT-mediated DC ablation and immunization, we observed that the primary and secondary antibody responses were equivalent in the presence of CD1d+ and CD1d DCs. In contrast, a total ablation of DCs delayed the primary antibody response. Further experiments revealed that depletion of CD1d+ DCs blocked in vivo expansion of antigen-specific cytotoxic (CD8+) T lymphocytes. These results provide a clear demonstration that although CD1d expression on DCs is essential for NKT-enhanced CD8+ T cell expansion, it is dispensable for specific antibody production.

Original languageEnglish (US)
Pages (from-to)783-790
Number of pages8
JournalJournal of Leukocyte Biology
Volume91
Issue number5
DOIs
StatePublished - May 1 2012
Externally publishedYes

Fingerprint

Natural Killer T-Cells
Humoral Immunity
Dendritic Cells
Antibody Formation
T-Lymphocytes
B-Lymphocytes
Viral Tumor Antigens
Glycolipids
Cytotoxic T-Lymphocytes
Immunization
Bone Marrow
Antigens
Peptides

Keywords

  • α-Galactosylceramide
  • Adaptive Immunity
  • Antibody
  • Antigen Presentation

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Differential contribution of dendritic cell CD1d to NKT cell-enhanced humoral immunity and CD8+ T cell activation. / Joshi, Sunil; Lang, Gillian A.; Devera, T. Scott; Johnson, Amy M.; Kovats, Susan; Lang, Mark L.

In: Journal of Leukocyte Biology, Vol. 91, No. 5, 01.05.2012, p. 783-790.

Research output: Contribution to journalArticle

Joshi, Sunil ; Lang, Gillian A. ; Devera, T. Scott ; Johnson, Amy M. ; Kovats, Susan ; Lang, Mark L. / Differential contribution of dendritic cell CD1d to NKT cell-enhanced humoral immunity and CD8+ T cell activation. In: Journal of Leukocyte Biology. 2012 ; Vol. 91, No. 5. pp. 783-790.
@article{38ef026bfb2246098f60d9da88daf62f,
title = "Differential contribution of dendritic cell CD1d to NKT cell-enhanced humoral immunity and CD8+ T cell activation",
abstract = "CD1d-restricted type I NKT cells provide help for specific antibody production. B cells, which have captured and presented a T-dependent, antigen-derived peptide on MHC class II and CD1d-binding glycolipid α-GC on CD1d, respectively, activate Th and NKT cells to elicit B cell help. However, the role of the DC CD1d in humoral immunity remains unknown. We therefore constructed mixed bone marrow chimeras containing CD1d-ex-pressing, DTR-transgenic DCs and CD1d+ or CD1d nontransgenic DCs. Following DT-mediated DC ablation and immunization, we observed that the primary and secondary antibody responses were equivalent in the presence of CD1d+ and CD1d DCs. In contrast, a total ablation of DCs delayed the primary antibody response. Further experiments revealed that depletion of CD1d+ DCs blocked in vivo expansion of antigen-specific cytotoxic (CD8+) T lymphocytes. These results provide a clear demonstration that although CD1d expression on DCs is essential for NKT-enhanced CD8+ T cell expansion, it is dispensable for specific antibody production.",
keywords = "α-Galactosylceramide, Adaptive Immunity, Antibody, Antigen Presentation",
author = "Sunil Joshi and Lang, {Gillian A.} and Devera, {T. Scott} and Johnson, {Amy M.} and Susan Kovats and Lang, {Mark L.}",
year = "2012",
month = "5",
day = "1",
doi = "10.1189/jlb.1111559",
language = "English (US)",
volume = "91",
pages = "783--790",
journal = "Journal of Leukocyte Biology",
issn = "0741-5400",
publisher = "FASEB",
number = "5",

}

TY - JOUR

T1 - Differential contribution of dendritic cell CD1d to NKT cell-enhanced humoral immunity and CD8+ T cell activation

AU - Joshi, Sunil

AU - Lang, Gillian A.

AU - Devera, T. Scott

AU - Johnson, Amy M.

AU - Kovats, Susan

AU - Lang, Mark L.

PY - 2012/5/1

Y1 - 2012/5/1

N2 - CD1d-restricted type I NKT cells provide help for specific antibody production. B cells, which have captured and presented a T-dependent, antigen-derived peptide on MHC class II and CD1d-binding glycolipid α-GC on CD1d, respectively, activate Th and NKT cells to elicit B cell help. However, the role of the DC CD1d in humoral immunity remains unknown. We therefore constructed mixed bone marrow chimeras containing CD1d-ex-pressing, DTR-transgenic DCs and CD1d+ or CD1d nontransgenic DCs. Following DT-mediated DC ablation and immunization, we observed that the primary and secondary antibody responses were equivalent in the presence of CD1d+ and CD1d DCs. In contrast, a total ablation of DCs delayed the primary antibody response. Further experiments revealed that depletion of CD1d+ DCs blocked in vivo expansion of antigen-specific cytotoxic (CD8+) T lymphocytes. These results provide a clear demonstration that although CD1d expression on DCs is essential for NKT-enhanced CD8+ T cell expansion, it is dispensable for specific antibody production.

AB - CD1d-restricted type I NKT cells provide help for specific antibody production. B cells, which have captured and presented a T-dependent, antigen-derived peptide on MHC class II and CD1d-binding glycolipid α-GC on CD1d, respectively, activate Th and NKT cells to elicit B cell help. However, the role of the DC CD1d in humoral immunity remains unknown. We therefore constructed mixed bone marrow chimeras containing CD1d-ex-pressing, DTR-transgenic DCs and CD1d+ or CD1d nontransgenic DCs. Following DT-mediated DC ablation and immunization, we observed that the primary and secondary antibody responses were equivalent in the presence of CD1d+ and CD1d DCs. In contrast, a total ablation of DCs delayed the primary antibody response. Further experiments revealed that depletion of CD1d+ DCs blocked in vivo expansion of antigen-specific cytotoxic (CD8+) T lymphocytes. These results provide a clear demonstration that although CD1d expression on DCs is essential for NKT-enhanced CD8+ T cell expansion, it is dispensable for specific antibody production.

KW - α-Galactosylceramide

KW - Adaptive Immunity

KW - Antibody

KW - Antigen Presentation

UR - http://www.scopus.com/inward/record.url?scp=84860911029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860911029&partnerID=8YFLogxK

U2 - 10.1189/jlb.1111559

DO - 10.1189/jlb.1111559

M3 - Article

C2 - 22331103

AN - SCOPUS:84860911029

VL - 91

SP - 783

EP - 790

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

IS - 5

ER -