Differential contribution of dendritic cell CD1d to NKT cell-enhanced humoral immunity and CD8+ T cell activation

Sunil K. Joshi, Gillian A. Lang, T. Scott Devera, Amy M. Johnson, Susan Kovats, Mark L. Lang

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

CD1d-restricted type I NKT cells provide help for specific antibody production. B cells, which have captured and presented a T-dependent, antigen-derived peptide on MHC class II and CD1d-binding glycolipid α-GC on CD1d, respectively, activate Th and NKT cells to elicit B cell help. However, the role of the DC CD1d in humoral immunity remains unknown. We therefore constructed mixed bone marrow chimeras containing CD1d-ex-pressing, DTR-transgenic DCs and CD1d+ or CD1d nontransgenic DCs. Following DT-mediated DC ablation and immunization, we observed that the primary and secondary antibody responses were equivalent in the presence of CD1d+ and CD1d DCs. In contrast, a total ablation of DCs delayed the primary antibody response. Further experiments revealed that depletion of CD1d+ DCs blocked in vivo expansion of antigen-specific cytotoxic (CD8+) T lymphocytes. These results provide a clear demonstration that although CD1d expression on DCs is essential for NKT-enhanced CD8+ T cell expansion, it is dispensable for specific antibody production.

Original languageEnglish (US)
Pages (from-to)783-790
Number of pages8
JournalJournal of Leukocyte Biology
Volume91
Issue number5
DOIs
StatePublished - May 1 2012
Externally publishedYes

Keywords

  • Adaptive Immunity
  • Antibody
  • Antigen Presentation
  • α-Galactosylceramide

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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