Abstract
CD1d-restricted type I NKT cells provide help for specific antibody production. B cells, which have captured and presented a T-dependent, antigen-derived peptide on MHC class II and CD1d-binding glycolipid α-GC on CD1d, respectively, activate Th and NKT cells to elicit B cell help. However, the role of the DC CD1d in humoral immunity remains unknown. We therefore constructed mixed bone marrow chimeras containing CD1d-ex-pressing, DTR-transgenic DCs and CD1d+ or CD1d nontransgenic DCs. Following DT-mediated DC ablation and immunization, we observed that the primary and secondary antibody responses were equivalent in the presence of CD1d+ and CD1d DCs. In contrast, a total ablation of DCs delayed the primary antibody response. Further experiments revealed that depletion of CD1d+ DCs blocked in vivo expansion of antigen-specific cytotoxic (CD8+) T lymphocytes. These results provide a clear demonstration that although CD1d expression on DCs is essential for NKT-enhanced CD8+ T cell expansion, it is dispensable for specific antibody production.
Original language | English (US) |
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Pages (from-to) | 783-790 |
Number of pages | 8 |
Journal | Journal of Leukocyte Biology |
Volume | 91 |
Issue number | 5 |
DOIs | |
State | Published - May 2012 |
Keywords
- Adaptive Immunity
- Antibody
- Antigen Presentation
- α-Galactosylceramide
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Cell Biology