Different molecular weight forms of opioid receptors revealed by polyclonal antibodies

Sabita Roy, Y. X. Zhu, N. M. Lee, H. H. Loh

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Polyclonal antibodies were raised against a purified opioid receptor from bovine brain (Cho, et. al., 1986), and shown to inhibit 3H-diprenorphine binding to this receptor in a dose-dependent fashion. These antibodies were then used to characterize opioid-binding material present in rat brain and in NG108-15 neuroblastoma-glioma hybrid cells. Western blot analysis revealed that the antibodies reacted with a single species of 58,000 molecular weight in rat brain membranes; this closely corresponds in size to the bovine opioid receptor used to raise the antibodies. In contrast, the polyclonal antibodies reacted with a 45,000 molecular weight species in NG108-15 neuroblastoma-glioma hybrid cells; moreover, this band was specifically reduced in NG108-15 cells in which opioid receptors had been down-regulated by incubation with D-ala2-D-leu5-enkephalin for 24 hours. Thus at least two distinct opioid receptor molecules have been identified, which have antigenic similarities.

Original languageEnglish (US)
Pages (from-to)237-244
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume150
Issue number1
DOIs
StatePublished - Jan 15 1988
Externally publishedYes

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Opioid Receptors
Molecular Weight
Molecular weight
Antibodies
Brain
Hybrid Cells
Neuroblastoma
Glioma
Rats
Diprenorphine
Enkephalins
Opioid Analgesics
Western Blotting
Membranes
Molecules

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Different molecular weight forms of opioid receptors revealed by polyclonal antibodies. / Roy, Sabita; Zhu, Y. X.; Lee, N. M.; Loh, H. H.

In: Biochemical and Biophysical Research Communications, Vol. 150, No. 1, 15.01.1988, p. 237-244.

Research output: Contribution to journalArticle

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