Differences in flow cytometry and 3H-thymidine analyses of perturbed human lymphocytes

A. Pollack, C. B. Bagwell, J. Hudson, G. L. Irvin

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A calf thymocyte crude aqueous extract was tested for DNA synthesis inhibitory activity using phytohemagglutinin-stimulated human peripheral blood lymphocytes. Inhibition of DNA synthesis was assayed using tritiated thymidine and flow cytometry. Although the calf thymocyte crude extract inhibited tritiated thymidine incorporation by over 50%, only very slight changes in the flow cytometric analysis were observed. When dibutyryl-cyclic adenosine monophosphate was used as an inhibitor, a correlation in terms of the inhibition of tritiated thymidine to the inhibition by flow cytometry was observed.

Original languageEnglish (US)
Pages (from-to)486-490
Number of pages5
JournalJournal of Histochemistry and Cytochemistry
Issue number1
StatePublished - Jan 1 1979


ASJC Scopus subject areas

  • Anatomy
  • Histology

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