Differences in flow cytometry and 3H-thymidine analyses of perturbed human lymphocytes

Alan Pollack, C. B. Bagwell, J. Hudson, G. L. Irvin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A calf thymocyte crude aqueous extract was tested for DNA synthesis inhibitory activity using phytohemagglutinin-stimulated human peripheral blood lymphocytes. Inhibition of DNA synthesis was assayed using tritiated thymidine and flow cytometry. Although the calf thymocyte crude extract inhibited tritiated thymidine incorporation by over 50%, only very slight changes in the flow cytometric analysis were observed. When dibutyryl-cyclic adenosine monophosphate was used as an inhibitor, a correlation in terms of the inhibition of tritiated thymidine to the inhibition by flow cytometry was observed.

Original languageEnglish
Pages (from-to)486-490
Number of pages5
JournalJournal of Histochemistry and Cytochemistry
Volume27
Issue number1
StatePublished - Jan 1 1979

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Thymidine
Flow Cytometry
Lymphocytes
Thymocytes
Complex Mixtures
DNA
Phytohemagglutinins
Cyclic AMP

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Differences in flow cytometry and 3H-thymidine analyses of perturbed human lymphocytes. / Pollack, Alan; Bagwell, C. B.; Hudson, J.; Irvin, G. L.

In: Journal of Histochemistry and Cytochemistry, Vol. 27, No. 1, 01.01.1979, p. 486-490.

Research output: Contribution to journalArticle

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