Dietary sodium inhibits aqueous copper uptake in rainbow trout (Oncorhynchus mykiss)

G. G. Pyle, C. N. Kamunde, D. G. McDonald, C. M. Wood

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Ours is the first study to demonstrate an influence of dietary sodium on waterborne copper uptake in fish. We examined possible interactions between dietary sodium and the response of freshwater rainbow trout (Oncorhynchus mykiss) to waterborne copper in light of recent evidence of interactions between sodium and copper metabolism in the gills. Trout were maintained for 6days on one of four diets of increasing sodium concentration (0.25 mmol g-1, 0.51 mmol g-1, 0.76 mmol g-1 and 1.27 mmolg-1, which corresponds to 0.6%, 1.2%, 1.8% and 3% sodium by mass, respectively). At the end of 7days, fish were exposed for 6h to waterborne copper spiked with 64Cu to determine if the dietary sodium affected responses to a subsequent short-term waterborne copper exposure. The radiotracer allowed us to distinguish between Cu occurring in fish tissues before the experiment and 'newly accumulated' Cu arising from the experimental exposure. Dietary sodium concentrations of 1.8% or 3% reduced newly accumulated copper concentrations in gill (from 93.9 ng g-1 in control to 38.9 ng g-1 and 20.0 ng g-1 in fish fed 1.8% or 3% Na+-supplemented diets, respectively), liver (from 64.3 ng g-1 to 23.1 ng g-1 and 7.5 ng g-1, respectively), kidney (from 29.3 ng g-1 to 11.7 ng g-1 and 7.8 ng g-1, respectively), plasma (from 64.7 ng g-1 to 21.5 ng g-1 and 10.7 ng g-1, respectively) and gut (from 6.8 ng g-1 to 3.4 ng g-1 and 2.2 ng g-1, respectively) by 50.0-88.2%. The 3% Na+-supplemented diets also increased plasma and gut sodium concentrations by 38.1% (from 137.1 μmol g-1 to 189.3 μmol g-1) and 104.3% (from 56.5 μmol g-1 to 115.4 μmol g-1), respectively, relative to fish maintained on untreated diets. Whole body uptake rates of both sodium and copper were significantly reduced, and highly correlated (r=0.97) with one another, in fish fed high-sodium diets relative to controls. Moreover, sodium efflux was 12% and 38% higher in fish fed 1.8% and 3% sodium-enriched diets, respectively. Fish fed high-sodium diets also drank more water, but the contribution of drinking to waterborne copper uptake was negligible. From these results, we speculate that, at least in part, aqueous sodium and copper share a common branchial uptake route, probably through an apical sodium channel. According to this hypothesis, as the channel is downregulated with increasing internal sodium concentrations, both sodium and copper uptake from the water are inhibited.

Original languageEnglish (US)
Pages (from-to)609-618
Number of pages10
JournalJournal of Experimental Biology
Volume206
Issue number3
DOIs
StatePublished - Feb 2003

Keywords

  • Aqueous copper uptake
  • Dietary sodium
  • Fish
  • Oncorhynchus mykiss
  • Rainbow trout

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

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