Dietary berries and ellagic acid prevent oxidative DNA damage and modulate expression of DNA repair genes

Harini S. Aiyer, Manicka V. Vadhanam, Radka Stoyanova, Gerard D. Caprio, Margie L. Clapper, Ramesh C. Gupta

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

DNA damage is a pre-requisite for the initiation of cancer and agents that reduce this damage are useful in cancer prevention. In this study, we evaluated the ability of whole berries and berry phytochemical, ellagic acid to reduce endogenous oxidative DNA damage. Ellagic acid was selected based on >95% inhibition of 8-oxodeoxyguosine (8-oxodG) and other unidentified oxidative DNA adducts induced by 4-hydroxy-17β-estradiol and CuCl 2 in vitro. Inhibition of the latter occurred at lower concentrations (10 μM) than that for 8-oxodG (100 μM). In the in vivo study, female CD-1 mice (n=6) were fed either a control diet or diet supplemented with ellagic acid (400 ppm) and dehydrated berries (5% w/w) with varying ellagic acid contents - blueberry (low), strawberry (medium) and red raspberry (high), for 3 weeks. Blueberry and strawberry diets showed moderate reductions in endogenous DNA adducts (25%). However, both red raspberry and ellagic acid diets showed a significant reduction of 59% (p <0.001) and 48% (p < 0.01), respectively. Both diets also resulted in a 3-8 fold over-expression of genes involved in DNA repair such as xeroderma pigmentosum group A complementing protein (XPA), DNA excision repair protein (ERCC5) and DNA ligase III (DNL3). These results suggest that red raspberry and ellagic acid reduce endogenous oxidative DNA damage by mechanisms which may involve increase in DNA repair.

Original languageEnglish (US)
Pages (from-to)327-341
Number of pages15
JournalInternational journal of molecular sciences
Volume9
Issue number3
DOIs
StatePublished - Mar 2008

Keywords

  • 4-Hydroxy estradiol
  • P-postlabeling
  • Dietary intervention
  • DNA damage and repair
  • Edible berries
  • Ellagic acid
  • Polyphenols
  • Raspberries

ASJC Scopus subject areas

  • Chemistry(all)
  • Molecular Biology

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