Diel variation in chlorophyll a, carbohydrate and protein content of the marine diatom Skeletonema costatum

G. L. Hitchcock

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Skeletonema costatum (Greville) Cleve isolated from Narragansett Bay, USA, was incubated at 3 light intensities (ca. 0.008, 0.040 and 0.075 ly min-1) under a 12 h light: 12 h dark (12L:12D) photoperiod at 2°, 10° and 20°C. Cellular chlorophyll a increased at intensities less than ca. 0.040 ly min-1; increases occured within one photoperiod at temperatures above 10°C. Cellular carbohydrate increased with light intensity at all temperatures; increases during the photophase were due to net production of the dilute acid-soluble fraction. Cellular protein increased during the photoperiod at 10° and 20°C; there was little difference in cellular protein among all cultures after one photoperiod. The rate at which cellular chlorophyll a increased in response to a decrease in light suggests that diel variation in cellular chlorophyll a is temperature-dependent in S. costatum. Protein: carbohydrate ratios ranged from ca. 0.5 to 2.0 over a diel cycle; ratios increased at lower intensities and higher temperatures. The diel range in protein:carbohydrate ratios equals that in cultures developing nitrogen deficiency; thus, use of this ratio as an index to phytoplankton physiological state must account for diel light effects.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalMarine Biology
Volume57
Issue number4
DOIs
StatePublished - Oct 1 1980

Fingerprint

diel variation
Skeletonema costatum
Bacillariophyceae
photoperiod
carbohydrate content
carbohydrate
chlorophyll a
diatom
protein content
chlorophyll
protein
carbohydrates
light intensity
temperature
proteins
light effect
photophase
physiological state
phytoplankton
acids

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Diel variation in chlorophyll a, carbohydrate and protein content of the marine diatom Skeletonema costatum. / Hitchcock, G. L.

In: Marine Biology, Vol. 57, No. 4, 01.10.1980, p. 271-278.

Research output: Contribution to journalArticle

@article{3ac68d25cd184a2bb2dd1845523deb00,
title = "Diel variation in chlorophyll a, carbohydrate and protein content of the marine diatom Skeletonema costatum",
abstract = "Skeletonema costatum (Greville) Cleve isolated from Narragansett Bay, USA, was incubated at 3 light intensities (ca. 0.008, 0.040 and 0.075 ly min-1) under a 12 h light: 12 h dark (12L:12D) photoperiod at 2°, 10° and 20°C. Cellular chlorophyll a increased at intensities less than ca. 0.040 ly min-1; increases occured within one photoperiod at temperatures above 10°C. Cellular carbohydrate increased with light intensity at all temperatures; increases during the photophase were due to net production of the dilute acid-soluble fraction. Cellular protein increased during the photoperiod at 10° and 20°C; there was little difference in cellular protein among all cultures after one photoperiod. The rate at which cellular chlorophyll a increased in response to a decrease in light suggests that diel variation in cellular chlorophyll a is temperature-dependent in S. costatum. Protein: carbohydrate ratios ranged from ca. 0.5 to 2.0 over a diel cycle; ratios increased at lower intensities and higher temperatures. The diel range in protein:carbohydrate ratios equals that in cultures developing nitrogen deficiency; thus, use of this ratio as an index to phytoplankton physiological state must account for diel light effects.",
author = "Hitchcock, {G. L.}",
year = "1980",
month = "10",
day = "1",
doi = "10.1007/BF00387570",
language = "English",
volume = "57",
pages = "271--278",
journal = "Marine Biology",
issn = "0025-3162",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Diel variation in chlorophyll a, carbohydrate and protein content of the marine diatom Skeletonema costatum

AU - Hitchcock, G. L.

PY - 1980/10/1

Y1 - 1980/10/1

N2 - Skeletonema costatum (Greville) Cleve isolated from Narragansett Bay, USA, was incubated at 3 light intensities (ca. 0.008, 0.040 and 0.075 ly min-1) under a 12 h light: 12 h dark (12L:12D) photoperiod at 2°, 10° and 20°C. Cellular chlorophyll a increased at intensities less than ca. 0.040 ly min-1; increases occured within one photoperiod at temperatures above 10°C. Cellular carbohydrate increased with light intensity at all temperatures; increases during the photophase were due to net production of the dilute acid-soluble fraction. Cellular protein increased during the photoperiod at 10° and 20°C; there was little difference in cellular protein among all cultures after one photoperiod. The rate at which cellular chlorophyll a increased in response to a decrease in light suggests that diel variation in cellular chlorophyll a is temperature-dependent in S. costatum. Protein: carbohydrate ratios ranged from ca. 0.5 to 2.0 over a diel cycle; ratios increased at lower intensities and higher temperatures. The diel range in protein:carbohydrate ratios equals that in cultures developing nitrogen deficiency; thus, use of this ratio as an index to phytoplankton physiological state must account for diel light effects.

AB - Skeletonema costatum (Greville) Cleve isolated from Narragansett Bay, USA, was incubated at 3 light intensities (ca. 0.008, 0.040 and 0.075 ly min-1) under a 12 h light: 12 h dark (12L:12D) photoperiod at 2°, 10° and 20°C. Cellular chlorophyll a increased at intensities less than ca. 0.040 ly min-1; increases occured within one photoperiod at temperatures above 10°C. Cellular carbohydrate increased with light intensity at all temperatures; increases during the photophase were due to net production of the dilute acid-soluble fraction. Cellular protein increased during the photoperiod at 10° and 20°C; there was little difference in cellular protein among all cultures after one photoperiod. The rate at which cellular chlorophyll a increased in response to a decrease in light suggests that diel variation in cellular chlorophyll a is temperature-dependent in S. costatum. Protein: carbohydrate ratios ranged from ca. 0.5 to 2.0 over a diel cycle; ratios increased at lower intensities and higher temperatures. The diel range in protein:carbohydrate ratios equals that in cultures developing nitrogen deficiency; thus, use of this ratio as an index to phytoplankton physiological state must account for diel light effects.

UR - http://www.scopus.com/inward/record.url?scp=0000231323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000231323&partnerID=8YFLogxK

U2 - 10.1007/BF00387570

DO - 10.1007/BF00387570

M3 - Article

AN - SCOPUS:0000231323

VL - 57

SP - 271

EP - 278

JO - Marine Biology

JF - Marine Biology

SN - 0025-3162

IS - 4

ER -