Dicopper(II) complexes showing DNA hydrolase activity and monomeric adduct formation with bis(4-nitrophenyl)phosphate

Mithun Roy; Shanta Dhar; Basudev Maity; Akhil R. Chakravarty

doi:10.1016/j.ica.2011.04.046

Dicopper(II) complexes showing DNA hydrolase activity and monomeric adduct formation with bis(4-nitrophenyl)phosphate

Mithun Roy, Shanta Dhar, Basudev Maity, Akhil R. Chakravarty

Research output: Contribution to journal › Article

13 Scopus citations

Abstract

Ferromagnetic dicopper(II) complexes [Cu₂(μ-O ₂CCH₃)(μ-OH)(L)₂(μ-L¹)] (PF₆)₂, where L = 1,10-phenanthroline (phen), L ¹ = H₂O in 1 and L = dipyrido[3,2-d:2′,3′-f] quinoxaline (dpq), L¹ = CH₃CN in 2, are prepared and structurally characterized. Crystals of 1 and 2 belong to the monoclinic space group of P2₁/n and P2₁/m, respectively. The copper(II) centers display distorted square-pyramidal geometry having a phenanthroline base and two oxygen atoms of the bridging hydroxo and acetate group in the basal plane. The fifth coordination site has weak axially bound bridging solvent molecule H₂O in 1 and CH₃CN in 2. The Cu·· ·Cu distances are 3.034 and 3.046 in 1 and 2, respectively. The complexes show efficient hydrolytic cleavage of supercoiled pUC19 DNA as evidenced from the mechanistic studies that include T4 DNA ligase experiments. The binuclear complexes form monomeric copper(II) adducts [Cu(L)₂(BNPP)](PF ₆) (L = phen, 3; dpq, 4) with bis(4-nitrophenyl)phosphate (BNPP) as a model phosphodiester. The crystal structures of 3 and 4 reveal distorted trigonal bipyramidal geometry in which BNPP binds through the oxygen atom of the phosphate. The kinetic data of the DNA cleavage reactions of the binuclear complexes under pseudo- and true-Michaelis-Menten conditions indicate remarkable enhancement in the DNA hydrolysis rate in comparison to the control data.

Original language	English (US)
Pages (from-to)	173-180
Number of pages	8
Journal	Inorganica Chimica Acta
Volume	375
Issue number	1
DOIs	https://doi.org/10.1016/j.ica.2011.04.046
State	Published - Sep 1 2011
Externally published	Yes

Keywords

Bis(4-nitrophenyl)phosphate adduct
Copper
Crystal structures
Hydrolytic DNA cleavage
Phenanthroline bases
Reaction kinetics

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Inorganic Chemistry
Materials Chemistry

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Roy, M., Dhar, S., Maity, B., & Chakravarty, A. R. (2011). Dicopper(II) complexes showing DNA hydrolase activity and monomeric adduct formation with bis(4-nitrophenyl)phosphate. Inorganica Chimica Acta, 375(1), 173-180. https://doi.org/10.1016/j.ica.2011.04.046

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title = "Dicopper(II) complexes showing DNA hydrolase activity and monomeric adduct formation with bis(4-nitrophenyl)phosphate",

abstract = "Ferromagnetic dicopper(II) complexes [Cu2(μ-O 2CCH3)(μ-OH)(L)2(μ-L1)] (PF6)2, where L = 1,10-phenanthroline (phen), L 1 = H2O in 1 and L = dipyrido[3,2-d:2′,3′-f] quinoxaline (dpq), L1 = CH3CN in 2, are prepared and structurally characterized. Crystals of 1 and 2 belong to the monoclinic space group of P21/n and P21/m, respectively. The copper(II) centers display distorted square-pyramidal geometry having a phenanthroline base and two oxygen atoms of the bridging hydroxo and acetate group in the basal plane. The fifth coordination site has weak axially bound bridging solvent molecule H2O in 1 and CH3CN in 2. The Cu·· ·Cu distances are 3.034 and 3.046 in 1 and 2, respectively. The complexes show efficient hydrolytic cleavage of supercoiled pUC19 DNA as evidenced from the mechanistic studies that include T4 DNA ligase experiments. The binuclear complexes form monomeric copper(II) adducts [Cu(L)2(BNPP)](PF 6) (L = phen, 3; dpq, 4) with bis(4-nitrophenyl)phosphate (BNPP) as a model phosphodiester. The crystal structures of 3 and 4 reveal distorted trigonal bipyramidal geometry in which BNPP binds through the oxygen atom of the phosphate. The kinetic data of the DNA cleavage reactions of the binuclear complexes under pseudo- and true-Michaelis-Menten conditions indicate remarkable enhancement in the DNA hydrolysis rate in comparison to the control data.",

keywords = "Bis(4-nitrophenyl)phosphate adduct, Copper, Crystal structures, Hydrolytic DNA cleavage, Phenanthroline bases, Reaction kinetics",

author = "Mithun Roy and Shanta Dhar and Basudev Maity and Chakravarty, {Akhil R.}",

year = "2011",

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doi = "10.1016/j.ica.2011.04.046",

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journal = "Inorganica Chimica Acta",

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T1 - Dicopper(II) complexes showing DNA hydrolase activity and monomeric adduct formation with bis(4-nitrophenyl)phosphate

AU - Roy, Mithun

AU - Dhar, Shanta

AU - Maity, Basudev

AU - Chakravarty, Akhil R.

PY - 2011/9/1

Y1 - 2011/9/1

N2 - Ferromagnetic dicopper(II) complexes [Cu2(μ-O 2CCH3)(μ-OH)(L)2(μ-L1)] (PF6)2, where L = 1,10-phenanthroline (phen), L 1 = H2O in 1 and L = dipyrido[3,2-d:2′,3′-f] quinoxaline (dpq), L1 = CH3CN in 2, are prepared and structurally characterized. Crystals of 1 and 2 belong to the monoclinic space group of P21/n and P21/m, respectively. The copper(II) centers display distorted square-pyramidal geometry having a phenanthroline base and two oxygen atoms of the bridging hydroxo and acetate group in the basal plane. The fifth coordination site has weak axially bound bridging solvent molecule H2O in 1 and CH3CN in 2. The Cu·· ·Cu distances are 3.034 and 3.046 in 1 and 2, respectively. The complexes show efficient hydrolytic cleavage of supercoiled pUC19 DNA as evidenced from the mechanistic studies that include T4 DNA ligase experiments. The binuclear complexes form monomeric copper(II) adducts [Cu(L)2(BNPP)](PF 6) (L = phen, 3; dpq, 4) with bis(4-nitrophenyl)phosphate (BNPP) as a model phosphodiester. The crystal structures of 3 and 4 reveal distorted trigonal bipyramidal geometry in which BNPP binds through the oxygen atom of the phosphate. The kinetic data of the DNA cleavage reactions of the binuclear complexes under pseudo- and true-Michaelis-Menten conditions indicate remarkable enhancement in the DNA hydrolysis rate in comparison to the control data.

AB - Ferromagnetic dicopper(II) complexes [Cu2(μ-O 2CCH3)(μ-OH)(L)2(μ-L1)] (PF6)2, where L = 1,10-phenanthroline (phen), L 1 = H2O in 1 and L = dipyrido[3,2-d:2′,3′-f] quinoxaline (dpq), L1 = CH3CN in 2, are prepared and structurally characterized. Crystals of 1 and 2 belong to the monoclinic space group of P21/n and P21/m, respectively. The copper(II) centers display distorted square-pyramidal geometry having a phenanthroline base and two oxygen atoms of the bridging hydroxo and acetate group in the basal plane. The fifth coordination site has weak axially bound bridging solvent molecule H2O in 1 and CH3CN in 2. The Cu·· ·Cu distances are 3.034 and 3.046 in 1 and 2, respectively. The complexes show efficient hydrolytic cleavage of supercoiled pUC19 DNA as evidenced from the mechanistic studies that include T4 DNA ligase experiments. The binuclear complexes form monomeric copper(II) adducts [Cu(L)2(BNPP)](PF 6) (L = phen, 3; dpq, 4) with bis(4-nitrophenyl)phosphate (BNPP) as a model phosphodiester. The crystal structures of 3 and 4 reveal distorted trigonal bipyramidal geometry in which BNPP binds through the oxygen atom of the phosphate. The kinetic data of the DNA cleavage reactions of the binuclear complexes under pseudo- and true-Michaelis-Menten conditions indicate remarkable enhancement in the DNA hydrolysis rate in comparison to the control data.

KW - Bis(4-nitrophenyl)phosphate adduct

KW - Copper

KW - Crystal structures

KW - Hydrolytic DNA cleavage

KW - Phenanthroline bases

KW - Reaction kinetics

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