Abstract
Ferromagnetic dicopper(II) complexes [Cu2(μ-O 2CCH3)(μ-OH)(L)2(μ-L1)] (PF6)2, where L = 1,10-phenanthroline (phen), L 1 = H2O in 1 and L = dipyrido[3,2-d:2′,3′-f] quinoxaline (dpq), L1 = CH3CN in 2, are prepared and structurally characterized. Crystals of 1 and 2 belong to the monoclinic space group of P21/n and P21/m, respectively. The copper(II) centers display distorted square-pyramidal geometry having a phenanthroline base and two oxygen atoms of the bridging hydroxo and acetate group in the basal plane. The fifth coordination site has weak axially bound bridging solvent molecule H2O in 1 and CH3CN in 2. The Cu·· ·Cu distances are 3.034 and 3.046 in 1 and 2, respectively. The complexes show efficient hydrolytic cleavage of supercoiled pUC19 DNA as evidenced from the mechanistic studies that include T4 DNA ligase experiments. The binuclear complexes form monomeric copper(II) adducts [Cu(L)2(BNPP)](PF 6) (L = phen, 3; dpq, 4) with bis(4-nitrophenyl)phosphate (BNPP) as a model phosphodiester. The crystal structures of 3 and 4 reveal distorted trigonal bipyramidal geometry in which BNPP binds through the oxygen atom of the phosphate. The kinetic data of the DNA cleavage reactions of the binuclear complexes under pseudo- and true-Michaelis-Menten conditions indicate remarkable enhancement in the DNA hydrolysis rate in comparison to the control data.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 173-180 |
| Number of pages | 8 |
| Journal | Inorganica Chimica Acta |
| Volume | 375 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 1 2011 |
| Externally published | Yes |
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Keywords
- Bis(4-nitrophenyl)phosphate adduct
- Copper
- Crystal structures
- Hydrolytic DNA cleavage
- Phenanthroline bases
- Reaction kinetics
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Inorganic Chemistry
- Materials Chemistry
Cite this
Dicopper(II) complexes showing DNA hydrolase activity and monomeric adduct formation with bis(4-nitrophenyl)phosphate. / Roy, Mithun; Dhar, Shanta; Maity, Basudev; Chakravarty, Akhil R.
In: Inorganica Chimica Acta, Vol. 375, No. 1, 01.09.2011, p. 173-180.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dicopper(II) complexes showing DNA hydrolase activity and monomeric adduct formation with bis(4-nitrophenyl)phosphate
AU - Roy, Mithun
AU - Dhar, Shanta
AU - Maity, Basudev
AU - Chakravarty, Akhil R.
PY - 2011/9/1
Y1 - 2011/9/1
N2 - Ferromagnetic dicopper(II) complexes [Cu2(μ-O 2CCH3)(μ-OH)(L)2(μ-L1)] (PF6)2, where L = 1,10-phenanthroline (phen), L 1 = H2O in 1 and L = dipyrido[3,2-d:2′,3′-f] quinoxaline (dpq), L1 = CH3CN in 2, are prepared and structurally characterized. Crystals of 1 and 2 belong to the monoclinic space group of P21/n and P21/m, respectively. The copper(II) centers display distorted square-pyramidal geometry having a phenanthroline base and two oxygen atoms of the bridging hydroxo and acetate group in the basal plane. The fifth coordination site has weak axially bound bridging solvent molecule H2O in 1 and CH3CN in 2. The Cu·· ·Cu distances are 3.034 and 3.046 in 1 and 2, respectively. The complexes show efficient hydrolytic cleavage of supercoiled pUC19 DNA as evidenced from the mechanistic studies that include T4 DNA ligase experiments. The binuclear complexes form monomeric copper(II) adducts [Cu(L)2(BNPP)](PF 6) (L = phen, 3; dpq, 4) with bis(4-nitrophenyl)phosphate (BNPP) as a model phosphodiester. The crystal structures of 3 and 4 reveal distorted trigonal bipyramidal geometry in which BNPP binds through the oxygen atom of the phosphate. The kinetic data of the DNA cleavage reactions of the binuclear complexes under pseudo- and true-Michaelis-Menten conditions indicate remarkable enhancement in the DNA hydrolysis rate in comparison to the control data.
AB - Ferromagnetic dicopper(II) complexes [Cu2(μ-O 2CCH3)(μ-OH)(L)2(μ-L1)] (PF6)2, where L = 1,10-phenanthroline (phen), L 1 = H2O in 1 and L = dipyrido[3,2-d:2′,3′-f] quinoxaline (dpq), L1 = CH3CN in 2, are prepared and structurally characterized. Crystals of 1 and 2 belong to the monoclinic space group of P21/n and P21/m, respectively. The copper(II) centers display distorted square-pyramidal geometry having a phenanthroline base and two oxygen atoms of the bridging hydroxo and acetate group in the basal plane. The fifth coordination site has weak axially bound bridging solvent molecule H2O in 1 and CH3CN in 2. The Cu·· ·Cu distances are 3.034 and 3.046 in 1 and 2, respectively. The complexes show efficient hydrolytic cleavage of supercoiled pUC19 DNA as evidenced from the mechanistic studies that include T4 DNA ligase experiments. The binuclear complexes form monomeric copper(II) adducts [Cu(L)2(BNPP)](PF 6) (L = phen, 3; dpq, 4) with bis(4-nitrophenyl)phosphate (BNPP) as a model phosphodiester. The crystal structures of 3 and 4 reveal distorted trigonal bipyramidal geometry in which BNPP binds through the oxygen atom of the phosphate. The kinetic data of the DNA cleavage reactions of the binuclear complexes under pseudo- and true-Michaelis-Menten conditions indicate remarkable enhancement in the DNA hydrolysis rate in comparison to the control data.
KW - Bis(4-nitrophenyl)phosphate adduct
KW - Copper
KW - Crystal structures
KW - Hydrolytic DNA cleavage
KW - Phenanthroline bases
KW - Reaction kinetics
UR - http://www.scopus.com/inward/record.url?scp=80051596781&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051596781&partnerID=8YFLogxK
U2 - 10.1016/j.ica.2011.04.046
DO - 10.1016/j.ica.2011.04.046
M3 - Article
AN - SCOPUS:80051596781
VL - 375
SP - 173
EP - 180
JO - Inorganica Chimica Acta
JF - Inorganica Chimica Acta
SN - 0020-1693
IS - 1
ER -