Diazepam-insensitive GABA(A) receptors on postnatal spiral ganglion neurones in culture

Brigitte Malgrange, Jean Michel Rigo, Philippe P. Lefebvre, Paul Coucke, Frédéric Goffin, Gaël Xhauflaire, Shibeshih Belachew, Thomas R. Van De Water, Gustave Moonen

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Using dissociated spiral ganglion cell cultures obtained from 3-day-old rat cochlea, we investigated the response of auditory neurones to γ-aminobutyric acid (GABA) using patch-clamp techniques. In our recording conditions, GABA elicited inward currents in > 95% of the neurones which reversed around 0 mV. Similar inward currents were measured using isoguvacin, a specific agonist of GABA(A) receptors. GABA-gated currents were reversibly inhibited by the channel blocker picrotoxin and the GABA competitive antagonist bicuculline. These functional GABA(A) receptors are characterized by an insensitivity to benzodiazepines and a relatively high sensitivity to β-carbolines and barbiturates. These results show that the GABA(A) receptor pharmacological properties of spiral ganglion neurones are close to those of cerebellar granule cells.

Original languageEnglish (US)
Pages (from-to)591-596
Number of pages6
JournalNeuroreport
Volume8
Issue number3
DOIs
StatePublished - Jan 1 1997

Keywords

  • afferent auditory neurones
  • benzodiazepines
  • GABA(A) receptor
  • patch-clamp

ASJC Scopus subject areas

  • Neuroscience(all)

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