Abstract
In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such a K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffi n-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffi n-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 45-65 |
| Number of pages | 21 |
| Journal | Methods in Molecular Biology |
| Volume | 1175 |
| DOIs | |
| State | Published - 2014 |
| Externally published | Yes |
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Keywords
- Diagnostic technique
- Paraffin tumoral tissue
- Personalized therapy
- Pharmacogenomic
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies. / Palmirotta, Raffaele; De Marchis, Maria Laura; Ludovici, Giorgia; Ferroni, Patrizia; Abete, Pasquale; Guadagni, Fiorella; Della Morte, David.
In: Methods in Molecular Biology, Vol. 1175, 2014, p. 45-65.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies
AU - Palmirotta, Raffaele
AU - De Marchis, Maria Laura
AU - Ludovici, Giorgia
AU - Ferroni, Patrizia
AU - Abete, Pasquale
AU - Guadagni, Fiorella
AU - Della Morte, David
PY - 2014
Y1 - 2014
N2 - In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such a K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffi n-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffi n-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.
AB - In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such a K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffi n-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffi n-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.
KW - Diagnostic technique
KW - Paraffin tumoral tissue
KW - Personalized therapy
KW - Pharmacogenomic
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UR - http://www.scopus.com/inward/citedby.url?scp=84927673730&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-0956-8_4
DO - 10.1007/978-1-4939-0956-8_4
M3 - Article
C2 - 25150866
AN - SCOPUS:84927673730
VL - 1175
SP - 45
EP - 65
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -