Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies

Raffaele Palmirotta; Maria Laura De Marchis; Giorgia Ludovici; Patrizia Ferroni; Pasquale Abete; Fiorella Guadagni; David Della Morte

doi:10.1007/978-1-4939-0956-8_4

Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies

Raffaele Palmirotta, Maria Laura De Marchis, Giorgia Ludovici, Patrizia Ferroni, Pasquale Abete, Fiorella Guadagni, David Della Morte

Research output: Contribution to journal › Article

2 Scopus citations

Abstract

In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such a K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffi n-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffi n-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.

Original language	English (US)
Pages (from-to)	45-65
Number of pages	21
Journal	Methods in Molecular Biology
Volume	1175
DOIs	https://doi.org/10.1007/978-1-4939-0956-8_4
State	Published - 2014
Externally published	Yes

Keywords

Diagnostic technique
Paraffin tumoral tissue
Personalized therapy
Pharmacogenomic

ASJC Scopus subject areas

Molecular Biology
Genetics

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Palmirotta, R., De Marchis, M. L., Ludovici, G., Ferroni, P., Abete, P., Guadagni, F., & Della Morte, D. (2014). Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies. Methods in Molecular Biology, 1175, 45-65. https://doi.org/10.1007/978-1-4939-0956-8_4

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abstract = "In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such a K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffi n-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffi n-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.",

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AU - Guadagni, Fiorella

AU - Della Morte, David

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