Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies

Raffaele Palmirotta, Maria Laura De Marchis, Giorgia Ludovici, Patrizia Ferroni, Pasquale Abete, Fiorella Guadagni, David Della Morte

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such a K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffi n-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffi n-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.

Original languageEnglish (US)
Pages (from-to)45-65
Number of pages21
JournalMethods in Molecular Biology
Volume1175
DOIs
StatePublished - 2014
Externally publishedYes

Keywords

  • Diagnostic technique
  • Paraffin tumoral tissue
  • Personalized therapy
  • Pharmacogenomic

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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