Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies

Raffaele Palmirotta; Maria Laura De Marchis; Giorgia Ludovici; Patrizia Ferroni; Pasquale Abete; Fiorella Guadagni; David Della Morte

doi:10.1007/978-1-4939-0956-8_4

Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies

Raffaele Palmirotta, Maria Laura De Marchis, Giorgia Ludovici, Patrizia Ferroni, Pasquale Abete, Fiorella Guadagni, David Della Morte

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such a K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffi n-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffi n-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.

Original language	English (US)
Pages (from-to)	45-65
Number of pages	21
Journal	Methods in Molecular Biology
Volume	1175
DOIs	https://doi.org/10.1007/978-1-4939-0956-8_4
State	Published - 2014
Externally published	Yes

Keywords

Diagnostic technique
Paraffin tumoral tissue
Personalized therapy
Pharmacogenomic

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-0956-8_4

Fingerprint Dive into the research topics of 'Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies'. Together they form a unique fingerprint.

Cite this

@article{0dbf0f2223fc4bf79e78b88515d281ec,

title = "Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies",

abstract = "In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such a K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffi n-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffi n-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.",

keywords = "Diagnostic technique, Paraffin tumoral tissue, Personalized therapy, Pharmacogenomic",

author = "Raffaele Palmirotta and {De Marchis}, {Maria Laura} and Giorgia Ludovici and Patrizia Ferroni and Pasquale Abete and Fiorella Guadagni and {Della Morte}, David",

year = "2014",

doi = "10.1007/978-1-4939-0956-8_4",

language = "English (US)",

volume = "1175",

pages = "45--65",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies

AU - Palmirotta, Raffaele

AU - De Marchis, Maria Laura

AU - Ludovici, Giorgia

AU - Ferroni, Patrizia

AU - Abete, Pasquale

AU - Guadagni, Fiorella

AU - Della Morte, David

PY - 2014

Y1 - 2014

N2 - In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such a K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffi n-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffi n-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.

AB - In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such a K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffi n-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffi n-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.

KW - Diagnostic technique

KW - Paraffin tumoral tissue

KW - Personalized therapy

KW - Pharmacogenomic

UR - http://www.scopus.com/inward/record.url?scp=84927673730&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84927673730&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-0956-8_4

DO - 10.1007/978-1-4939-0956-8_4

M3 - Article

C2 - 25150866

AN - SCOPUS:84927673730

VL - 1175

SP - 45

EP - 65

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

SN - 1064-3745

ER -

Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this