Diagnosis of toxoplasma parasitemia in patients with AIDS by gene detection after amplification with polymerase chain reaction

G. A. Filice, J. A. Hitt, Charles D Mitchell, M. Blackstad, S. W. Sorensen

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We sought evidence of toxoplasma parasitemia among 37 people with active or dormant Toxoplasma gondii infection or no evidence of infection. DNA was extracted from erythrocyte-free portions of blood samples, and the T. gondii B1 gene was amplified by the polymerase chain reaction. Evidence of T. gondii parasitemia was found in six patients with severe immunosuppression from AIDS and clinical evidence suggestive of or compatible with toxoplasmosis. Results were negative for patients unlikely to have active toxoplasmosis. Gene detection after amplification with the polymerase chain reaction is a promising test for detection of parasitemia, and parasitemia should be tested for in patients with AIDS and unexplained fever or central nervous system abnormalities.

Original languageEnglish
Pages (from-to)2327-2331
Number of pages5
JournalJournal of Clinical Microbiology
Volume31
Issue number9
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Parasitemia
Toxoplasma
Toxoplasmosis
Acquired Immunodeficiency Syndrome
Polymerase Chain Reaction
Genes
Nervous System Malformations
Immunosuppression
Fever
Central Nervous System
Erythrocytes
DNA
Infection

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Diagnosis of toxoplasma parasitemia in patients with AIDS by gene detection after amplification with polymerase chain reaction. / Filice, G. A.; Hitt, J. A.; Mitchell, Charles D; Blackstad, M.; Sorensen, S. W.

In: Journal of Clinical Microbiology, Vol. 31, No. 9, 01.01.1993, p. 2327-2331.

Research output: Contribution to journalArticle

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