Diagnosis of human immunodeficiency virus type 1 infection in infants by immune complex dissociation p24 assay

M. O. Paul, G. Toedter, D. Hofheinz, S. Tetali, S. Pelton, M. Marecki, A. Brena, E. J. Abrams, S. Landesman, S. Pahwa

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Using immune complex dissociation (ICD), we retrospectively examined serum and plasma of 206 infants aged 0 to 4 months who were perinatally exposed to human immunodeficiency virus (HIV). All samples were analyzed in a blinded manner. Infection status was determined based on the results of HIV culture and Centers for Disease Control and Prevention classification. The overall diagnostic sensitivity of the assay was 59% (93 samples, 73 infants), and specificity was 100% (160 samples, 133 infants). When the samples were analyzed according to age, sensitivity was highest at age 1 to 2 months (17 of 21 infants, 81%). Sensitivities at other ages were 53% at < 1 month, 55% at 2 to 3 months, and 48% at 3 to 4 months (9 of 17, 11 of 20, and 12 of 25 cases, respectively). In 11 evaluable cases there was a possible correlation of p24 antigen quantitation (in picograms per milliliter) with disease progression. We conclude that, as determined in this study, the ICD p24 is a rapid diagnostic assay for HIV infection with a sensitivity of > 80% at 1 to 2 months of age and 100% specificity, as evaluated, up to 4 months of age.

Original languageEnglish (US)
Pages (from-to)75-78
Number of pages4
JournalClinical and Diagnostic Laboratory Immunology
Volume4
Issue number1
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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    Paul, M. O., Toedter, G., Hofheinz, D., Tetali, S., Pelton, S., Marecki, M., Brena, A., Abrams, E. J., Landesman, S., & Pahwa, S. (1997). Diagnosis of human immunodeficiency virus type 1 infection in infants by immune complex dissociation p24 assay. Clinical and Diagnostic Laboratory Immunology, 4(1), 75-78. https://doi.org/10.1128/cdli.4.1.75-78.1997