Diagnosis of human immunodeficiency virus infection using an immunoglobulin E-based assay

Maryann Fletcher, Maria J. Miguez-Burbano, Gail Shor-Posner, Viola Lopez, Hong Lai, Marianna K. Baum

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Immunoglobulin assays that are sensitive and specific for detecting human immunodeficiency virus type 1 (HIV-1) infection are especially important in developing countries where PCR and vital culture may not be readily available. Immunoglobulin E (IgE), which is elevated in HIV-1 infection, is the only antibody that does not cross the placenta, making it potentially valuable for vital detection in both children and adults. This study developed an assay for detection of HIV specific IgE antibodies in adults. A total of 170 serum samples from 170 adults (116 HIV positive and 54 HIV negative) were analyzed. Serum or plasma samples were treated by using the protein G affinity method. The HIV status was determined by using two IgG enzyme-linked immunosorbent assays (ELISAs) and one Western blot evaluation. The IgE enzyme immunoassay test for HIV-1 correctly identified the HIV status in 98.8% of the samples (168 of 170). One false-positive and one false- negative test occurred with the IgE ELISA, as well as with the IgG ELISA test but were correctly identified by the IgE test. Analysis of the data demonstrated a high specificity (99%) and sensitivity (99%) of the IgE test, with 95% confidence intervals. The IgE assay appears to be sensitive and specific, suggesting that IgE-specific antibodies offer an effective method to detect HIV-1 infection in adults.

Original languageEnglish
Pages (from-to)55-57
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume7
Issue number1
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Virus Diseases
Viruses
Immunoglobulin E
Assays
HIV
Immunosorbents
HIV-1
Enzyme-Linked Immunosorbent Assay
Enzymes
Antibodies
Immunoglobulin G
Serum
Immunoenzyme Techniques
Developing countries
Placenta
Developing Countries
Immunoglobulins
Western Blotting
Confidence Intervals
Plasmas

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

Fletcher, M., Miguez-Burbano, M. J., Shor-Posner, G., Lopez, V., Lai, H., & Baum, M. K. (2000). Diagnosis of human immunodeficiency virus infection using an immunoglobulin E-based assay. Clinical and Diagnostic Laboratory Immunology, 7(1), 55-57.

Diagnosis of human immunodeficiency virus infection using an immunoglobulin E-based assay. / Fletcher, Maryann; Miguez-Burbano, Maria J.; Shor-Posner, Gail; Lopez, Viola; Lai, Hong; Baum, Marianna K.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 7, No. 1, 01.01.2000, p. 55-57.

Research output: Contribution to journalArticle

Fletcher, M, Miguez-Burbano, MJ, Shor-Posner, G, Lopez, V, Lai, H & Baum, MK 2000, 'Diagnosis of human immunodeficiency virus infection using an immunoglobulin E-based assay', Clinical and Diagnostic Laboratory Immunology, vol. 7, no. 1, pp. 55-57.
Fletcher M, Miguez-Burbano MJ, Shor-Posner G, Lopez V, Lai H, Baum MK. Diagnosis of human immunodeficiency virus infection using an immunoglobulin E-based assay. Clinical and Diagnostic Laboratory Immunology. 2000 Jan 1;7(1):55-57.
Fletcher, Maryann ; Miguez-Burbano, Maria J. ; Shor-Posner, Gail ; Lopez, Viola ; Lai, Hong ; Baum, Marianna K. / Diagnosis of human immunodeficiency virus infection using an immunoglobulin E-based assay. In: Clinical and Diagnostic Laboratory Immunology. 2000 ; Vol. 7, No. 1. pp. 55-57.
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