Diagnosis of hepatitis C virus (HCV) infection using an immunodominant chimeric polyprotein to capture circulating antibodies: Reevaluation of the role of HCV in liver disease

D. Y. Chien, Q. L. Choo, A. Tabrizi, C. Kuo, J. McFarland, K. Berger, C. Lee, J. R. Shuster, Tuan Nguyen, D. L. Moyer, M. Tong, S. Furuta, M. Omata, G. Tegtmeier, H. Alter, Eugene R Schiff, Lennox J Jeffers, M. Houghton, G. Kuo

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Structural and nonstructural regions of the HCV-encoded polyprotein have been expressed in recombinant yeast, bacteria, or insect cells and used to capture and measure reactive antibodies circulating in different individuals. The putative nucleocapsid protein (C) and nonstructural proteins 3-5 (NS3-NS5) were found to contain the most immunodominant epitopes. The NS3, NS4, and C regions were expressed in yeast in the form of a fused, chimeric polyprotein (C25) and a capture assay for reactive antibody was developed. This anti-C25 assay detects all previously identified HCV-seropositive cases and provides a substantially more sensitive diagnostic for both acute and chronic HCV infections than the current anti-C100-3 (NS4) assay. Anti-C25 was detected more frequently than anti-C100-3 in chronic, transfusion-associated non-A, non-B hepatitis patients from the United States (95% vs. 71%) and Japan (98% vs. 82%), in cryptogenic cirrhosis patients from the United States (62% vs. 28%), and in hepatitis B surface antigen-negative cases of hepatocellular carcinoma from Japan (83% vs. 63%). These data indicate that HCV has a greater role in these liver diseases than was previously thought. In volunteer United States blood donors sampled following the introduction of anti-C100-3 screening, the prevalence of anti-C25 and anti-C100-3 was 0.5% and 0.08%, respectively.

Original languageEnglish
Pages (from-to)10011-10015
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number21
StatePublished - Dec 1 1992

Fingerprint

Polyproteins
Virus Diseases
Hepacivirus
Liver Diseases
Antibodies
Japan
Yeasts
Nucleocapsid Proteins
Immunodominant Epitopes
Chronic Hepatitis C
Hepatitis B Surface Antigens
Protein C
Blood Donors
Hepatitis
Insects
Volunteers
Hepatocellular Carcinoma
Bacteria
anti-C100-3
Proteins

Keywords

  • Blood screening
  • Enzyme immunoassay
  • Enzyme-linked immunosorbent assay
  • Non-A, non-B hepatitis

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Diagnosis of hepatitis C virus (HCV) infection using an immunodominant chimeric polyprotein to capture circulating antibodies : Reevaluation of the role of HCV in liver disease. / Chien, D. Y.; Choo, Q. L.; Tabrizi, A.; Kuo, C.; McFarland, J.; Berger, K.; Lee, C.; Shuster, J. R.; Nguyen, Tuan; Moyer, D. L.; Tong, M.; Furuta, S.; Omata, M.; Tegtmeier, G.; Alter, H.; Schiff, Eugene R; Jeffers, Lennox J; Houghton, M.; Kuo, G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 21, 01.12.1992, p. 10011-10015.

Research output: Contribution to journalArticle

Chien, DY, Choo, QL, Tabrizi, A, Kuo, C, McFarland, J, Berger, K, Lee, C, Shuster, JR, Nguyen, T, Moyer, DL, Tong, M, Furuta, S, Omata, M, Tegtmeier, G, Alter, H, Schiff, ER, Jeffers, LJ, Houghton, M & Kuo, G 1992, 'Diagnosis of hepatitis C virus (HCV) infection using an immunodominant chimeric polyprotein to capture circulating antibodies: Reevaluation of the role of HCV in liver disease', Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 21, pp. 10011-10015.
Chien, D. Y. ; Choo, Q. L. ; Tabrizi, A. ; Kuo, C. ; McFarland, J. ; Berger, K. ; Lee, C. ; Shuster, J. R. ; Nguyen, Tuan ; Moyer, D. L. ; Tong, M. ; Furuta, S. ; Omata, M. ; Tegtmeier, G. ; Alter, H. ; Schiff, Eugene R ; Jeffers, Lennox J ; Houghton, M. ; Kuo, G. / Diagnosis of hepatitis C virus (HCV) infection using an immunodominant chimeric polyprotein to capture circulating antibodies : Reevaluation of the role of HCV in liver disease. In: Proceedings of the National Academy of Sciences of the United States of America. 1992 ; Vol. 89, No. 21. pp. 10011-10015.
@article{b0633a3d1e874c188573603f285dda89,
title = "Diagnosis of hepatitis C virus (HCV) infection using an immunodominant chimeric polyprotein to capture circulating antibodies: Reevaluation of the role of HCV in liver disease",
abstract = "Structural and nonstructural regions of the HCV-encoded polyprotein have been expressed in recombinant yeast, bacteria, or insect cells and used to capture and measure reactive antibodies circulating in different individuals. The putative nucleocapsid protein (C) and nonstructural proteins 3-5 (NS3-NS5) were found to contain the most immunodominant epitopes. The NS3, NS4, and C regions were expressed in yeast in the form of a fused, chimeric polyprotein (C25) and a capture assay for reactive antibody was developed. This anti-C25 assay detects all previously identified HCV-seropositive cases and provides a substantially more sensitive diagnostic for both acute and chronic HCV infections than the current anti-C100-3 (NS4) assay. Anti-C25 was detected more frequently than anti-C100-3 in chronic, transfusion-associated non-A, non-B hepatitis patients from the United States (95{\%} vs. 71{\%}) and Japan (98{\%} vs. 82{\%}), in cryptogenic cirrhosis patients from the United States (62{\%} vs. 28{\%}), and in hepatitis B surface antigen-negative cases of hepatocellular carcinoma from Japan (83{\%} vs. 63{\%}). These data indicate that HCV has a greater role in these liver diseases than was previously thought. In volunteer United States blood donors sampled following the introduction of anti-C100-3 screening, the prevalence of anti-C25 and anti-C100-3 was 0.5{\%} and 0.08{\%}, respectively.",
keywords = "Blood screening, Enzyme immunoassay, Enzyme-linked immunosorbent assay, Non-A, non-B hepatitis",
author = "Chien, {D. Y.} and Choo, {Q. L.} and A. Tabrizi and C. Kuo and J. McFarland and K. Berger and C. Lee and Shuster, {J. R.} and Tuan Nguyen and Moyer, {D. L.} and M. Tong and S. Furuta and M. Omata and G. Tegtmeier and H. Alter and Schiff, {Eugene R} and Jeffers, {Lennox J} and M. Houghton and G. Kuo",
year = "1992",
month = "12",
day = "1",
language = "English",
volume = "89",
pages = "10011--10015",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "21",

}

TY - JOUR

T1 - Diagnosis of hepatitis C virus (HCV) infection using an immunodominant chimeric polyprotein to capture circulating antibodies

T2 - Reevaluation of the role of HCV in liver disease

AU - Chien, D. Y.

AU - Choo, Q. L.

AU - Tabrizi, A.

AU - Kuo, C.

AU - McFarland, J.

AU - Berger, K.

AU - Lee, C.

AU - Shuster, J. R.

AU - Nguyen, Tuan

AU - Moyer, D. L.

AU - Tong, M.

AU - Furuta, S.

AU - Omata, M.

AU - Tegtmeier, G.

AU - Alter, H.

AU - Schiff, Eugene R

AU - Jeffers, Lennox J

AU - Houghton, M.

AU - Kuo, G.

PY - 1992/12/1

Y1 - 1992/12/1

N2 - Structural and nonstructural regions of the HCV-encoded polyprotein have been expressed in recombinant yeast, bacteria, or insect cells and used to capture and measure reactive antibodies circulating in different individuals. The putative nucleocapsid protein (C) and nonstructural proteins 3-5 (NS3-NS5) were found to contain the most immunodominant epitopes. The NS3, NS4, and C regions were expressed in yeast in the form of a fused, chimeric polyprotein (C25) and a capture assay for reactive antibody was developed. This anti-C25 assay detects all previously identified HCV-seropositive cases and provides a substantially more sensitive diagnostic for both acute and chronic HCV infections than the current anti-C100-3 (NS4) assay. Anti-C25 was detected more frequently than anti-C100-3 in chronic, transfusion-associated non-A, non-B hepatitis patients from the United States (95% vs. 71%) and Japan (98% vs. 82%), in cryptogenic cirrhosis patients from the United States (62% vs. 28%), and in hepatitis B surface antigen-negative cases of hepatocellular carcinoma from Japan (83% vs. 63%). These data indicate that HCV has a greater role in these liver diseases than was previously thought. In volunteer United States blood donors sampled following the introduction of anti-C100-3 screening, the prevalence of anti-C25 and anti-C100-3 was 0.5% and 0.08%, respectively.

AB - Structural and nonstructural regions of the HCV-encoded polyprotein have been expressed in recombinant yeast, bacteria, or insect cells and used to capture and measure reactive antibodies circulating in different individuals. The putative nucleocapsid protein (C) and nonstructural proteins 3-5 (NS3-NS5) were found to contain the most immunodominant epitopes. The NS3, NS4, and C regions were expressed in yeast in the form of a fused, chimeric polyprotein (C25) and a capture assay for reactive antibody was developed. This anti-C25 assay detects all previously identified HCV-seropositive cases and provides a substantially more sensitive diagnostic for both acute and chronic HCV infections than the current anti-C100-3 (NS4) assay. Anti-C25 was detected more frequently than anti-C100-3 in chronic, transfusion-associated non-A, non-B hepatitis patients from the United States (95% vs. 71%) and Japan (98% vs. 82%), in cryptogenic cirrhosis patients from the United States (62% vs. 28%), and in hepatitis B surface antigen-negative cases of hepatocellular carcinoma from Japan (83% vs. 63%). These data indicate that HCV has a greater role in these liver diseases than was previously thought. In volunteer United States blood donors sampled following the introduction of anti-C100-3 screening, the prevalence of anti-C25 and anti-C100-3 was 0.5% and 0.08%, respectively.

KW - Blood screening

KW - Enzyme immunoassay

KW - Enzyme-linked immunosorbent assay

KW - Non-A, non-B hepatitis

UR - http://www.scopus.com/inward/record.url?scp=0026448768&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026448768&partnerID=8YFLogxK

M3 - Article

C2 - 1279666

AN - SCOPUS:0026448768

VL - 89

SP - 10011

EP - 10015

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 21

ER -