Diagnosis of glycogen storage disease type I by serum immunodiffusion

P. J. Collipp, S. Y. Chen, V. T. Maddaiah, J. Thomas, F. J. Huijing

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

An antiserum was prepared by immunizing guinea pigs with a preparation of partially purified human liver microsomal glucose 6 phosphatase. Using double agar diffusion and immunoelectrophoresis this antiserum was found to react with an antigen in normal human serum, and also with serum from a child with glycogen storage disease, type III, i.e., debranching enzyme deficiency. No reaction occurred with serum or liver from patients with glycogen storage disease type I. While additional experience with this method is needed, this paper presents a potential method for establishing the diagnosis of type I glycogen storage disease without resort to liver biopsy.

Original languageEnglish (US)
Pages (from-to)71-75
Number of pages5
JournalPediatrics
Volume53
Issue number1
StatePublished - Jan 1 1974

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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