Diabetes is not associated with a change in the elemental composition of the pancreatic B cell in diabetic C57BL KsJ-db/db mice

Lisa Juntti-Berggren, Ulf Lindh, Per Olof Berggren, Ove Berglund, Barbara J. Frankel

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Freeze-dried pancreas sections from 7-, 17-and 27-week-old genetically diabetic (db/db) and normal (±/±) mice were subjected to proton bombardment and the concentrations of 15 elements in B cells and exocrine pancreas were calculated from the characteristic X-rays emitted. In the 7-week-old diabetic animals, B cells contained significantly above-normal levels of Na and S, while exocrine pancreas contained subnormal levels of Ca, and excess Mn. The B cells from the 17-week-old diabetic animals contained subnormal levels of Cu and the exocrine pancreas of the 27-week-old diabetic animals was deficient in Cd. The 7-, 17- and 27-week-old, genetically diabetic (db/db) mice were hyperglycemic, hyperinsulinemic and heavier than age-matched normal (±/±) mice. Although significant changes were found in elemental composition when comparing both B cells and exocrine pancreas at different ages, the changes were not consistent. Therefore, it appears as if the measured elemental changes were random and not related to the onset of diabetes.

Original languageEnglish
Pages (from-to)217-223
Number of pages7
JournalBioscience Reports
Volume10
Issue number2
DOIs
StatePublished - Apr 1 1990
Externally publishedYes

Fingerprint

Exocrine Pancreas
Insulin-Secreting Cells
Medical problems
B-Lymphocytes
Cells
Animals
Chemical analysis
Obese Mice
Protons
Pancreas
X-Rays
X rays

Keywords

  • C57BL KsJ-db/db mice
  • diabetes mellitus
  • exocrine pancreas
  • Islets of Langerhans
  • pancreatic B cell
  • proton microprobe analysis

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Diabetes is not associated with a change in the elemental composition of the pancreatic B cell in diabetic C57BL KsJ-db/db mice. / Juntti-Berggren, Lisa; Lindh, Ulf; Berggren, Per Olof; Berglund, Ove; Frankel, Barbara J.

In: Bioscience Reports, Vol. 10, No. 2, 01.04.1990, p. 217-223.

Research output: Contribution to journalArticle

Juntti-Berggren, Lisa ; Lindh, Ulf ; Berggren, Per Olof ; Berglund, Ove ; Frankel, Barbara J. / Diabetes is not associated with a change in the elemental composition of the pancreatic B cell in diabetic C57BL KsJ-db/db mice. In: Bioscience Reports. 1990 ; Vol. 10, No. 2. pp. 217-223.
@article{cb34e8ef0ff242ff9116b4144730182a,
title = "Diabetes is not associated with a change in the elemental composition of the pancreatic B cell in diabetic C57BL KsJ-db/db mice",
abstract = "Freeze-dried pancreas sections from 7-, 17-and 27-week-old genetically diabetic (db/db) and normal (±/±) mice were subjected to proton bombardment and the concentrations of 15 elements in B cells and exocrine pancreas were calculated from the characteristic X-rays emitted. In the 7-week-old diabetic animals, B cells contained significantly above-normal levels of Na and S, while exocrine pancreas contained subnormal levels of Ca, and excess Mn. The B cells from the 17-week-old diabetic animals contained subnormal levels of Cu and the exocrine pancreas of the 27-week-old diabetic animals was deficient in Cd. The 7-, 17- and 27-week-old, genetically diabetic (db/db) mice were hyperglycemic, hyperinsulinemic and heavier than age-matched normal (±/±) mice. Although significant changes were found in elemental composition when comparing both B cells and exocrine pancreas at different ages, the changes were not consistent. Therefore, it appears as if the measured elemental changes were random and not related to the onset of diabetes.",
keywords = "C57BL KsJ-db/db mice, diabetes mellitus, exocrine pancreas, Islets of Langerhans, pancreatic B cell, proton microprobe analysis",
author = "Lisa Juntti-Berggren and Ulf Lindh and Berggren, {Per Olof} and Ove Berglund and Frankel, {Barbara J.}",
year = "1990",
month = "4",
day = "1",
doi = "10.1007/BF01116581",
language = "English",
volume = "10",
pages = "217--223",
journal = "Bioscience Reports",
issn = "0144-8463",
publisher = "Portland Press Ltd.",
number = "2",

}

TY - JOUR

T1 - Diabetes is not associated with a change in the elemental composition of the pancreatic B cell in diabetic C57BL KsJ-db/db mice

AU - Juntti-Berggren, Lisa

AU - Lindh, Ulf

AU - Berggren, Per Olof

AU - Berglund, Ove

AU - Frankel, Barbara J.

PY - 1990/4/1

Y1 - 1990/4/1

N2 - Freeze-dried pancreas sections from 7-, 17-and 27-week-old genetically diabetic (db/db) and normal (±/±) mice were subjected to proton bombardment and the concentrations of 15 elements in B cells and exocrine pancreas were calculated from the characteristic X-rays emitted. In the 7-week-old diabetic animals, B cells contained significantly above-normal levels of Na and S, while exocrine pancreas contained subnormal levels of Ca, and excess Mn. The B cells from the 17-week-old diabetic animals contained subnormal levels of Cu and the exocrine pancreas of the 27-week-old diabetic animals was deficient in Cd. The 7-, 17- and 27-week-old, genetically diabetic (db/db) mice were hyperglycemic, hyperinsulinemic and heavier than age-matched normal (±/±) mice. Although significant changes were found in elemental composition when comparing both B cells and exocrine pancreas at different ages, the changes were not consistent. Therefore, it appears as if the measured elemental changes were random and not related to the onset of diabetes.

AB - Freeze-dried pancreas sections from 7-, 17-and 27-week-old genetically diabetic (db/db) and normal (±/±) mice were subjected to proton bombardment and the concentrations of 15 elements in B cells and exocrine pancreas were calculated from the characteristic X-rays emitted. In the 7-week-old diabetic animals, B cells contained significantly above-normal levels of Na and S, while exocrine pancreas contained subnormal levels of Ca, and excess Mn. The B cells from the 17-week-old diabetic animals contained subnormal levels of Cu and the exocrine pancreas of the 27-week-old diabetic animals was deficient in Cd. The 7-, 17- and 27-week-old, genetically diabetic (db/db) mice were hyperglycemic, hyperinsulinemic and heavier than age-matched normal (±/±) mice. Although significant changes were found in elemental composition when comparing both B cells and exocrine pancreas at different ages, the changes were not consistent. Therefore, it appears as if the measured elemental changes were random and not related to the onset of diabetes.

KW - C57BL KsJ-db/db mice

KW - diabetes mellitus

KW - exocrine pancreas

KW - Islets of Langerhans

KW - pancreatic B cell

KW - proton microprobe analysis

UR - http://www.scopus.com/inward/record.url?scp=0025330143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025330143&partnerID=8YFLogxK

U2 - 10.1007/BF01116581

DO - 10.1007/BF01116581

M3 - Article

C2 - 2192765

AN - SCOPUS:0025330143

VL - 10

SP - 217

EP - 223

JO - Bioscience Reports

JF - Bioscience Reports

SN - 0144-8463

IS - 2

ER -