Dexamethasone Protects Against Radiation-induced Loss of Auditory Hair Cells In Vitro

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Abstract

HYPOTHESIS:: Dexamethasone (DXM) protects against radiation-induced loss of auditory hair cells (HCs) in rat organ of Corti (OC) explants by reducing levels of oxidative stress and apoptosis. BACKGROUND:: Radiation-induced sensorineural hearing loss (HL) is progressive, dose-dependent, and irreversible. Currently, there are no preventative therapeutic modalities for radiation-induced HL. DXM is a synthetic steroid that can potentially target many of the pathways involved in radiation-induced ototoxicity. METHODS:: Whole OC explants were dissected from 3-day-old rat cochleae exposed to specific dosages of single-fraction radiation (0, 2, 5, 10, or 20?Gy), were either untreated or treated with DXM (75, 150, 300?μg/mL), and then cultured for 48 or 96?hours. Confocal microscopy for oxidative stress (CellRox, 48?h) and apoptosis (TUNEL assay, 96?h) and fluorescent microscopy for viable HC counts (fluorescein isothiocyanate-phalloidin, 96?h) were performed. Analysis of variance and Tukey post hoc testing were used for statistical analysis. RESULTS:: Radiation exposure initiated dose-dependent losses of inner and outer HCs, predominantly in the basal turns of the OC explants. DXM protected against radiation-induced HC losses in a dose-dependent manner. DXM significantly reduced levels of oxidative stress and apoptosis in radiation-injured OC explants (p?

Original languageEnglish (US)
JournalOtology and Neurotology
DOIs
StateAccepted/In press - Sep 29 2015

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Clinical Neurology
  • Sensory Systems

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