Dexamethasone attenuates NF-κB DNA binding activity without inducing IκB levels in rat brain in vivo

M. Tino Unlap, Richard S Jope

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

This investigation tested if glucocorticoid hormones modulate activation of the NF-κB transcription factor in rat brain in vivo. Dexamethasone (2 mg/kg) administration decreased NF-κB DNA binding in cerebral cortex and hippocampus nuclear extracts, maximally at 3-6 h after dexamethasone, followed by recovery at 24 h. Dexamethasone did not inhibit NF-κB by increasing the level of the inhibitory protein IκBα, as occurs in some peripheral cells, but instead lowered IκBα levels. Direct protein-protein inhibition by glucocorticoids was indicated by co-precipitation of glucocorticoid receptors with the p65 NF-κB subunit. Thus, glucocorticoids inhibit NF-κB DNA binding in rat brain, apparently by complexing with NF-κB subunits, which may contribute to the detrimental effects of glucocorticoids on neuronal function associated with oxidative stress and excitotoxicity.

Original languageEnglish
Pages (from-to)83-89
Number of pages7
JournalMolecular Brain Research
Volume45
Issue number1
DOIs
StatePublished - Apr 1 1997
Externally publishedYes

Fingerprint

Dexamethasone
Glucocorticoids
DNA
Brain
Proteins
Glucocorticoid Receptors
Cerebral Cortex
Hippocampus
Oxidative Stress
Transcription Factors
Hormones

Keywords

  • AP-1
  • Glucocorticoid
  • IκB
  • NF-κB
  • Oxidative stress

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Dexamethasone attenuates NF-κB DNA binding activity without inducing IκB levels in rat brain in vivo. / Unlap, M. Tino; Jope, Richard S.

In: Molecular Brain Research, Vol. 45, No. 1, 01.04.1997, p. 83-89.

Research output: Contribution to journalArticle

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