Dexamethasone and methylprednisolone do not inhibit neuritic outgrowth while inhibiting outgrowth of fibroblasts from spiral ganglion explants

Alexis Furze, Deanna Kralick, Anand Vakharia, Korey Jaben, Reid Graves, Eelam Adil, Adrien Eshraghi, Thomas J. Balkany, Thomas R Van De Water

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Conclusion. Dexamethasone and methylprednisolone do not inhibit neuritic outgrowth while inhibiting fibroblastic outgrowth from spiral ganglion micro-explants. Objectives. To demonstrate reduced fibroblastic outgrowth while maintaining neurite outgrowth for several corticosteroids using an in vitro test system of neonatal rat spiral ganglion micro-explants. Materials and methods. The in vitro test system comprised 3-day-old rat spiral ganglion micro-explants. Dexamethasone, methylprednisolone, triamcinolone acetonide, and human recombinant brain-derived neurotrophic factor (hrBDNF) were tested in vitro. The control was ganglion micro-explants in supplemented Dulbecco's modified Eagle's medium. Areas of the ganglion explant, neurite and fibroblast outgrowth of ganglion explants after 10 days in vitro were imaged, digitized, and analyzed using Image Tool 3.00 on a PC workstation. Areas of neurite and fibroblast outgrowth from the experimental explants were compared against values obtained from control explants. Results. Dexamethasone gave the best result of the three corticosteroids tested for inhibiting fibroblast outgrowth while not inhibiting neurite outgrowth from the ganglion micro-explants. Media containing hrBDNF (10 ng/ml) stimulated significantly greater neurite outgrowth than outgrowth from control explants (p<0.001). Ganglion micro-explants treated with dexamethasone (0.02 mg/ml) and methylprednisolone (0.5 mg/ml) provided the greatest inhibition of fibroblast outgrowth compared with control explants (p<0.001).

Original languageEnglish
Pages (from-to)122-127
Number of pages6
JournalActa Oto-Laryngologica
Volume128
Issue number2
DOIs
StatePublished - Jan 24 2008

Fingerprint

Spiral Ganglion
Methylprednisolone
Ganglia
Dexamethasone
Fibroblasts
Adrenal Cortex Hormones
Triamcinolone Acetonide
Eagles
Neuronal Outgrowth
In Vitro Techniques

Keywords

  • Auditory neurons
  • Cochlear implantation
  • Dexamethasone
  • Fibroblast outgrowth
  • hrBDNF
  • Methylprednisolone
  • Neurite outgrowth
  • Spiral ganglion
  • Triamcinolone acetonide

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Dexamethasone and methylprednisolone do not inhibit neuritic outgrowth while inhibiting outgrowth of fibroblasts from spiral ganglion explants. / Furze, Alexis; Kralick, Deanna; Vakharia, Anand; Jaben, Korey; Graves, Reid; Adil, Eelam; Eshraghi, Adrien; Balkany, Thomas J.; Van De Water, Thomas R.

In: Acta Oto-Laryngologica, Vol. 128, No. 2, 24.01.2008, p. 122-127.

Research output: Contribution to journalArticle

Furze, Alexis ; Kralick, Deanna ; Vakharia, Anand ; Jaben, Korey ; Graves, Reid ; Adil, Eelam ; Eshraghi, Adrien ; Balkany, Thomas J. ; Van De Water, Thomas R. / Dexamethasone and methylprednisolone do not inhibit neuritic outgrowth while inhibiting outgrowth of fibroblasts from spiral ganglion explants. In: Acta Oto-Laryngologica. 2008 ; Vol. 128, No. 2. pp. 122-127.
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abstract = "Conclusion. Dexamethasone and methylprednisolone do not inhibit neuritic outgrowth while inhibiting fibroblastic outgrowth from spiral ganglion micro-explants. Objectives. To demonstrate reduced fibroblastic outgrowth while maintaining neurite outgrowth for several corticosteroids using an in vitro test system of neonatal rat spiral ganglion micro-explants. Materials and methods. The in vitro test system comprised 3-day-old rat spiral ganglion micro-explants. Dexamethasone, methylprednisolone, triamcinolone acetonide, and human recombinant brain-derived neurotrophic factor (hrBDNF) were tested in vitro. The control was ganglion micro-explants in supplemented Dulbecco's modified Eagle's medium. Areas of the ganglion explant, neurite and fibroblast outgrowth of ganglion explants after 10 days in vitro were imaged, digitized, and analyzed using Image Tool 3.00 on a PC workstation. Areas of neurite and fibroblast outgrowth from the experimental explants were compared against values obtained from control explants. Results. Dexamethasone gave the best result of the three corticosteroids tested for inhibiting fibroblast outgrowth while not inhibiting neurite outgrowth from the ganglion micro-explants. Media containing hrBDNF (10 ng/ml) stimulated significantly greater neurite outgrowth than outgrowth from control explants (p<0.001). Ganglion micro-explants treated with dexamethasone (0.02 mg/ml) and methylprednisolone (0.5 mg/ml) provided the greatest inhibition of fibroblast outgrowth compared with control explants (p<0.001).",
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AU - Furze, Alexis

AU - Kralick, Deanna

AU - Vakharia, Anand

AU - Jaben, Korey

AU - Graves, Reid

AU - Adil, Eelam

AU - Eshraghi, Adrien

AU - Balkany, Thomas J.

AU - Van De Water, Thomas R

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N2 - Conclusion. Dexamethasone and methylprednisolone do not inhibit neuritic outgrowth while inhibiting fibroblastic outgrowth from spiral ganglion micro-explants. Objectives. To demonstrate reduced fibroblastic outgrowth while maintaining neurite outgrowth for several corticosteroids using an in vitro test system of neonatal rat spiral ganglion micro-explants. Materials and methods. The in vitro test system comprised 3-day-old rat spiral ganglion micro-explants. Dexamethasone, methylprednisolone, triamcinolone acetonide, and human recombinant brain-derived neurotrophic factor (hrBDNF) were tested in vitro. The control was ganglion micro-explants in supplemented Dulbecco's modified Eagle's medium. Areas of the ganglion explant, neurite and fibroblast outgrowth of ganglion explants after 10 days in vitro were imaged, digitized, and analyzed using Image Tool 3.00 on a PC workstation. Areas of neurite and fibroblast outgrowth from the experimental explants were compared against values obtained from control explants. Results. Dexamethasone gave the best result of the three corticosteroids tested for inhibiting fibroblast outgrowth while not inhibiting neurite outgrowth from the ganglion micro-explants. Media containing hrBDNF (10 ng/ml) stimulated significantly greater neurite outgrowth than outgrowth from control explants (p<0.001). Ganglion micro-explants treated with dexamethasone (0.02 mg/ml) and methylprednisolone (0.5 mg/ml) provided the greatest inhibition of fibroblast outgrowth compared with control explants (p<0.001).

AB - Conclusion. Dexamethasone and methylprednisolone do not inhibit neuritic outgrowth while inhibiting fibroblastic outgrowth from spiral ganglion micro-explants. Objectives. To demonstrate reduced fibroblastic outgrowth while maintaining neurite outgrowth for several corticosteroids using an in vitro test system of neonatal rat spiral ganglion micro-explants. Materials and methods. The in vitro test system comprised 3-day-old rat spiral ganglion micro-explants. Dexamethasone, methylprednisolone, triamcinolone acetonide, and human recombinant brain-derived neurotrophic factor (hrBDNF) were tested in vitro. The control was ganglion micro-explants in supplemented Dulbecco's modified Eagle's medium. Areas of the ganglion explant, neurite and fibroblast outgrowth of ganglion explants after 10 days in vitro were imaged, digitized, and analyzed using Image Tool 3.00 on a PC workstation. Areas of neurite and fibroblast outgrowth from the experimental explants were compared against values obtained from control explants. Results. Dexamethasone gave the best result of the three corticosteroids tested for inhibiting fibroblast outgrowth while not inhibiting neurite outgrowth from the ganglion micro-explants. Media containing hrBDNF (10 ng/ml) stimulated significantly greater neurite outgrowth than outgrowth from control explants (p<0.001). Ganglion micro-explants treated with dexamethasone (0.02 mg/ml) and methylprednisolone (0.5 mg/ml) provided the greatest inhibition of fibroblast outgrowth compared with control explants (p<0.001).

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KW - Fibroblast outgrowth

KW - hrBDNF

KW - Methylprednisolone

KW - Neurite outgrowth

KW - Spiral ganglion

KW - Triamcinolone acetonide

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