Developmental regulation of IL-4, IL-2, and IFN-γ production by murine peripheral T lymphocytes

Rebecca D Adkins, Arman Ghanei, Kara Hamilton

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Abstract

Lymph node T cells from naive, 4-day-old neonatal mice resemble activated adult T cells in that they produce low levels of IL-2 but high levels of IL-4 in response to anti-CD3 stimulation in vitro. Herein, we show that the capacity for high level IL-4 production is rapidly lost in the early postnatal period. A decline is first evident at 5 days postbirth. By 6 days postbirth, T cells secrete IL-4 at low levels, similar to those produced by T cells from naive, adult animals. In contrast, the acquisition of high, adultlike IL-2 or IFN-γ production does not occur until approximately 6 wk of life. Thus, the loss of high level IL-4 production and the acquisition of high level IL-2 and IFN-γ production are distinct developmental events, occurring at widely separated intervals. There are two phases in the transition to adultlike IL-2 production. In the early neonatal period, the underproduction of IL-2 appears to be due to a combination of intrinsic nonresponsiveness to CD3-mediated stimulation and the production of a soluble inhibitor of IL-2 production. The inhibitory activity required the presence of IL-4 because neutralizing anti-IL-4 antibody completely eliminated inhibition. Moreover, experiments using IL-4 showed that IL-4 a one was sufficient to dramatically inhibit IL-2 production by T cells from naive, adult animals in a primary stimulation. Between 4 to 5 days and 6 wk of life, the underproduction of IL-2 and IFN-γ appears to result solely from a lowered responsiveness of the T cells. Thus, the progression to adultlike lymphokine production involves a combination of changes in the types of lymphokines produced and in the magnitude of the response to activation signals.

Original languageEnglish
Pages (from-to)6617-6626
Number of pages10
JournalJournal of Immunology
Volume151
Issue number12
StatePublished - Dec 15 1993

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Interleukin-4
Interleukin-2
T-Lymphocytes
Lymphokines
Phase Transition
Lymph Nodes
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Developmental regulation of IL-4, IL-2, and IFN-γ production by murine peripheral T lymphocytes. / Adkins, Rebecca D; Ghanei, Arman; Hamilton, Kara.

In: Journal of Immunology, Vol. 151, No. 12, 15.12.1993, p. 6617-6626.

Research output: Contribution to journalArticle

Adkins, RD, Ghanei, A & Hamilton, K 1993, 'Developmental regulation of IL-4, IL-2, and IFN-γ production by murine peripheral T lymphocytes', Journal of Immunology, vol. 151, no. 12, pp. 6617-6626.
Adkins, Rebecca D ; Ghanei, Arman ; Hamilton, Kara. / Developmental regulation of IL-4, IL-2, and IFN-γ production by murine peripheral T lymphocytes. In: Journal of Immunology. 1993 ; Vol. 151, No. 12. pp. 6617-6626.
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