Developmental potential of CD4-8- thymocytes. Peripheral progeny include mature CD4-8- T cells bearing αβ T cell receptor

C. J. Guidos, I. L. Weissman, Rebecca D Adkins

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

We have used the intra-thymic transfer system to investigate the population dynamics of thymocyte and mature T cell subsets in the absence of continuing precursor input from the bone marrow. We have followed the development and life span of CD4+ and CD8+ thymocyte subsets and mature peripheral T cells from intra-thymically injected adult or fetal CD4-8- thymic precursors. both precursor types proliferated, differentiated, and exported to peripheral lymphoid tissues αβ-TCR+CD4+8- and CD4-8+ progeny which formed a stable, long-lived component of the peripheral T cell pool. The production of phenotypically mature thymocytes and peripheral T cells occurred more rapidly from fetal CD4-8- precursors. CD4+8-:CD4-8+ ratios among peripheral progeny of intra-thymically-injected CD4-8- precursors were initially normal, but they steadily declined among progeny of the fetal precursors. Thus, there appear to be differences in the life span and/or proliferative capacity of mature T cells derived from embryonic vs adult progenitors. In addition to the predominant CD4+8- and CD48+ subsets of peripheral T cells, a minor (1 to 20%) population of Thy-1+CD3+4-8- T cells was identified among peripheral progeny of intra-thymically-injected CD4-8- thymocytes, as well as in lymph nodes of unmanipulated animals. A total of 20 to 34% of this subset expressed V(β8)+ TCR and the majority were CD5(hi), Pgp-1+, and J11d-. The function and specificity of this newly identified population of thymically derived peripheral T cells remains to be investigated.

Original languageEnglish
Pages (from-to)3773-3780
Number of pages8
JournalJournal of Immunology
Volume142
Issue number11
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Thymocytes
T-Cell Antigen Receptor
T-Lymphocytes
T-Lymphocyte Subsets
Population Dynamics
Lymphoid Tissue
Population
Lymph Nodes
Bone Marrow

ASJC Scopus subject areas

  • Immunology

Cite this

Developmental potential of CD4-8- thymocytes. Peripheral progeny include mature CD4-8- T cells bearing αβ T cell receptor. / Guidos, C. J.; Weissman, I. L.; Adkins, Rebecca D.

In: Journal of Immunology, Vol. 142, No. 11, 01.01.1989, p. 3773-3780.

Research output: Contribution to journalArticle

Guidos, C. J. ; Weissman, I. L. ; Adkins, Rebecca D. / Developmental potential of CD4-8- thymocytes. Peripheral progeny include mature CD4-8- T cells bearing αβ T cell receptor. In: Journal of Immunology. 1989 ; Vol. 142, No. 11. pp. 3773-3780.
@article{bf81646a9cfb400cbbe1700725a83f20,
title = "Developmental potential of CD4-8- thymocytes. Peripheral progeny include mature CD4-8- T cells bearing αβ T cell receptor",
abstract = "We have used the intra-thymic transfer system to investigate the population dynamics of thymocyte and mature T cell subsets in the absence of continuing precursor input from the bone marrow. We have followed the development and life span of CD4+ and CD8+ thymocyte subsets and mature peripheral T cells from intra-thymically injected adult or fetal CD4-8- thymic precursors. both precursor types proliferated, differentiated, and exported to peripheral lymphoid tissues αβ-TCR+CD4+8- and CD4-8+ progeny which formed a stable, long-lived component of the peripheral T cell pool. The production of phenotypically mature thymocytes and peripheral T cells occurred more rapidly from fetal CD4-8- precursors. CD4+8-:CD4-8+ ratios among peripheral progeny of intra-thymically-injected CD4-8- precursors were initially normal, but they steadily declined among progeny of the fetal precursors. Thus, there appear to be differences in the life span and/or proliferative capacity of mature T cells derived from embryonic vs adult progenitors. In addition to the predominant CD4+8- and CD48+ subsets of peripheral T cells, a minor (1 to 20{\%}) population of Thy-1+CD3+4-8- T cells was identified among peripheral progeny of intra-thymically-injected CD4-8- thymocytes, as well as in lymph nodes of unmanipulated animals. A total of 20 to 34{\%} of this subset expressed V(β8)+ TCR and the majority were CD5(hi), Pgp-1+, and J11d-. The function and specificity of this newly identified population of thymically derived peripheral T cells remains to be investigated.",
author = "Guidos, {C. J.} and Weissman, {I. L.} and Adkins, {Rebecca D}",
year = "1989",
month = "1",
day = "1",
language = "English",
volume = "142",
pages = "3773--3780",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "11",

}

TY - JOUR

T1 - Developmental potential of CD4-8- thymocytes. Peripheral progeny include mature CD4-8- T cells bearing αβ T cell receptor

AU - Guidos, C. J.

AU - Weissman, I. L.

AU - Adkins, Rebecca D

PY - 1989/1/1

Y1 - 1989/1/1

N2 - We have used the intra-thymic transfer system to investigate the population dynamics of thymocyte and mature T cell subsets in the absence of continuing precursor input from the bone marrow. We have followed the development and life span of CD4+ and CD8+ thymocyte subsets and mature peripheral T cells from intra-thymically injected adult or fetal CD4-8- thymic precursors. both precursor types proliferated, differentiated, and exported to peripheral lymphoid tissues αβ-TCR+CD4+8- and CD4-8+ progeny which formed a stable, long-lived component of the peripheral T cell pool. The production of phenotypically mature thymocytes and peripheral T cells occurred more rapidly from fetal CD4-8- precursors. CD4+8-:CD4-8+ ratios among peripheral progeny of intra-thymically-injected CD4-8- precursors were initially normal, but they steadily declined among progeny of the fetal precursors. Thus, there appear to be differences in the life span and/or proliferative capacity of mature T cells derived from embryonic vs adult progenitors. In addition to the predominant CD4+8- and CD48+ subsets of peripheral T cells, a minor (1 to 20%) population of Thy-1+CD3+4-8- T cells was identified among peripheral progeny of intra-thymically-injected CD4-8- thymocytes, as well as in lymph nodes of unmanipulated animals. A total of 20 to 34% of this subset expressed V(β8)+ TCR and the majority were CD5(hi), Pgp-1+, and J11d-. The function and specificity of this newly identified population of thymically derived peripheral T cells remains to be investigated.

AB - We have used the intra-thymic transfer system to investigate the population dynamics of thymocyte and mature T cell subsets in the absence of continuing precursor input from the bone marrow. We have followed the development and life span of CD4+ and CD8+ thymocyte subsets and mature peripheral T cells from intra-thymically injected adult or fetal CD4-8- thymic precursors. both precursor types proliferated, differentiated, and exported to peripheral lymphoid tissues αβ-TCR+CD4+8- and CD4-8+ progeny which formed a stable, long-lived component of the peripheral T cell pool. The production of phenotypically mature thymocytes and peripheral T cells occurred more rapidly from fetal CD4-8- precursors. CD4+8-:CD4-8+ ratios among peripheral progeny of intra-thymically-injected CD4-8- precursors were initially normal, but they steadily declined among progeny of the fetal precursors. Thus, there appear to be differences in the life span and/or proliferative capacity of mature T cells derived from embryonic vs adult progenitors. In addition to the predominant CD4+8- and CD48+ subsets of peripheral T cells, a minor (1 to 20%) population of Thy-1+CD3+4-8- T cells was identified among peripheral progeny of intra-thymically-injected CD4-8- thymocytes, as well as in lymph nodes of unmanipulated animals. A total of 20 to 34% of this subset expressed V(β8)+ TCR and the majority were CD5(hi), Pgp-1+, and J11d-. The function and specificity of this newly identified population of thymically derived peripheral T cells remains to be investigated.

UR - http://www.scopus.com/inward/record.url?scp=0024344037&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024344037&partnerID=8YFLogxK

M3 - Article

C2 - 2785564

AN - SCOPUS:0024344037

VL - 142

SP - 3773

EP - 3780

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 11

ER -