Developmental changes in the tracheal mucociliary system in neonatal sheep

R. J. Phipps, W. M. Abraham, A. T. Mariassy, P. J. Torrealba, M. W. Sielczak, A. Ahmed, M. McCray, J. S. Stevenson, Adam Wanner

Research output: Contribution to journalArticle

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Abstract

We studied the postnatal development of the tracheal epithelium and mucociliary system in neonatal sheep. Secretion of macromolecules (radiolabeled with 35SO4 and [3H]-threonine), unidirectional fluxes of Cl-, Na+, and water (measured with radioactive tracers), and ciliary beat frequency (CBF) were measured in tracheal tissues in vitro. Tracheal mucus transport velocity (TMV) was measured in vivo. Sheep were studied at 0, 2, 4, 8, and > 24 (adult) wk after birth. In newborn sheep trachea, secretion of macromolecules was significantly elevated (cf. adults), and there was basal net secretion of Cl- under short-circuit and open-circuit conditions. This induced open-circuit secretion of Na+. Secretion of macromolecules decreased rapidly by 2 wk (by 40-50%) and was not different from adult values by 4 wk. Active Na+ absorption developed rapidly, and from 2 wk onward it predominated under open-circuit conditions, inducing net Cl- absorption. These changes in secretory function were associated with an age-related increase in TMV, whereas inherent tracheal CBF was unchanged. In sheep, therefore, the newborn's trachea has elevated secretion of macromolecules and secretes Cl- and liquid under basal conditions. Normal secretory function (a reduction in secretion of macromolecules coupled with net absorption of ions and presumably of liquid also) approaches adult function by 2-4 wk of age.

Original languageEnglish
Pages (from-to)824-832
Number of pages9
JournalJournal of Applied Physiology
Volume67
Issue number2
StatePublished - Jan 1 1989

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Sheep
Mucus
Trachea
Radioactive Tracers
Threonine
Epithelium
Parturition
Ions

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Phipps, R. J., Abraham, W. M., Mariassy, A. T., Torrealba, P. J., Sielczak, M. W., Ahmed, A., ... Wanner, A. (1989). Developmental changes in the tracheal mucociliary system in neonatal sheep. Journal of Applied Physiology, 67(2), 824-832.

Developmental changes in the tracheal mucociliary system in neonatal sheep. / Phipps, R. J.; Abraham, W. M.; Mariassy, A. T.; Torrealba, P. J.; Sielczak, M. W.; Ahmed, A.; McCray, M.; Stevenson, J. S.; Wanner, Adam.

In: Journal of Applied Physiology, Vol. 67, No. 2, 01.01.1989, p. 824-832.

Research output: Contribution to journalArticle

Phipps, RJ, Abraham, WM, Mariassy, AT, Torrealba, PJ, Sielczak, MW, Ahmed, A, McCray, M, Stevenson, JS & Wanner, A 1989, 'Developmental changes in the tracheal mucociliary system in neonatal sheep', Journal of Applied Physiology, vol. 67, no. 2, pp. 824-832.
Phipps RJ, Abraham WM, Mariassy AT, Torrealba PJ, Sielczak MW, Ahmed A et al. Developmental changes in the tracheal mucociliary system in neonatal sheep. Journal of Applied Physiology. 1989 Jan 1;67(2):824-832.
Phipps, R. J. ; Abraham, W. M. ; Mariassy, A. T. ; Torrealba, P. J. ; Sielczak, M. W. ; Ahmed, A. ; McCray, M. ; Stevenson, J. S. ; Wanner, Adam. / Developmental changes in the tracheal mucociliary system in neonatal sheep. In: Journal of Applied Physiology. 1989 ; Vol. 67, No. 2. pp. 824-832.
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