Development of instrumentation and fluorochromes for automated multiparameter analysis of cells

R. C. Leif, R. A. Thomas, T. A. Yopp, B. D. Watson, V. R. Guarino, D. H. Hindman, N. Lefkove, L. M. Vallarino

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.

Original languageEnglish (US)
Pages (from-to)1492-1498
Number of pages7
JournalUnknown Journal
Volume23
Issue number8
DOIs
StatePublished - Jan 1 1977

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Fingerprint Dive into the research topics of 'Development of instrumentation and fluorochromes for automated multiparameter analysis of cells'. Together they form a unique fingerprint.

  • Cite this

    Leif, R. C., Thomas, R. A., Yopp, T. A., Watson, B. D., Guarino, V. R., Hindman, D. H., Lefkove, N., & Vallarino, L. M. (1977). Development of instrumentation and fluorochromes for automated multiparameter analysis of cells. Unknown Journal, 23(8), 1492-1498. https://doi.org/10.1093/clinchem/23.8.1492