Abstract
We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.
Original language | English (US) |
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Pages (from-to) | 1492-1498 |
Number of pages | 7 |
Journal | Unknown Journal |
Volume | 23 |
Issue number | 8 |
DOIs | |
State | Published - Jan 1 1977 |
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical