Abstract
We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.
| Original language | English |
|---|---|
| Pages (from-to) | 1492-1498 |
| Number of pages | 7 |
| Journal | Clinical Chemistry |
| Volume | 23 |
| Issue number | 8 |
| State | Published - Dec 1 1977 |
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ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Development of instrumentation and fluorochromes for automated multiparameter analysis of cells. / Leif, R. C.; Thomas, R. A.; Yopp, T. A.; Watson, B. D.; Guarino, V. R.; Hindman, D. H.; Lefkove, N.; Vallarino, L. M.
In: Clinical Chemistry, Vol. 23, No. 8, 01.12.1977, p. 1492-1498.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of instrumentation and fluorochromes for automated multiparameter analysis of cells
AU - Leif, R. C.
AU - Thomas, R. A.
AU - Yopp, T. A.
AU - Watson, B. D.
AU - Guarino, V. R.
AU - Hindman, D. H.
AU - Lefkove, N.
AU - Vallarino, L. M.
PY - 1977/12/1
Y1 - 1977/12/1
N2 - We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.
AB - We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.
UR - http://www.scopus.com/inward/record.url?scp=0017642057&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017642057&partnerID=8YFLogxK
M3 - Article
C2 - 872409
AN - SCOPUS:0017642057
VL - 23
SP - 1492
EP - 1498
JO - Clinical Chemistry
JF - Clinical Chemistry
SN - 0009-9147
IS - 8
ER -