Development of instrumentation and fluorochromes for automated multiparameter analysis of cells

R. C. Leif; R. A. Thomas; T. A. Yopp; B. D. Watson; V. R. Guarino; D. H. Hindman; N. Lefkove; L. M. Vallarino

doi:10.1093/clinchem/23.8.1492

Development of instrumentation and fluorochromes for automated multiparameter analysis of cells

R. C. Leif, R. A. Thomas, T. A. Yopp, B. D. Watson, V. R. Guarino, D. H. Hindman, N. Lefkove, L. M. Vallarino

Neurology

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.

Original language	English (US)
Pages (from-to)	1492-1498
Number of pages	7
Journal	Unknown Journal
Volume	23
Issue number	8
DOIs	https://doi.org/10.1093/clinchem/23.8.1492
State	Published - Jan 1 1977

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

Access to Document

10.1093/clinchem/23.8.1492

Fingerprint Dive into the research topics of 'Development of instrumentation and fluorochromes for automated multiparameter analysis of cells'. Together they form a unique fingerprint.

Cite this

@article{6fa85fe9a83147dbba7d079d31ad94f1,

title = "Development of instrumentation and fluorochromes for automated multiparameter analysis of cells",

abstract = "We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.",

author = "Leif, {R. C.} and Thomas, {R. A.} and Yopp, {T. A.} and Watson, {B. D.} and Guarino, {V. R.} and Hindman, {D. H.} and N. Lefkove and Vallarino, {L. M.}",

year = "1977",

month = jan,

day = "1",

doi = "10.1093/clinchem/23.8.1492",

language = "English (US)",

volume = "23",

pages = "1492--1498",

journal = "Scientific Computing and Instrumentation",

issn = "1078-8956",

publisher = "Springer Wien",

number = "8",

}

TY - JOUR

T1 - Development of instrumentation and fluorochromes for automated multiparameter analysis of cells

AU - Leif, R. C.

AU - Thomas, R. A.

AU - Yopp, T. A.

AU - Watson, B. D.

AU - Guarino, V. R.

AU - Hindman, D. H.

AU - Lefkove, N.

AU - Vallarino, L. M.

PY - 1977/1/1

Y1 - 1977/1/1

N2 - We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.

AB - We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.

UR - http://www.scopus.com/inward/record.url?scp=0017642057&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017642057&partnerID=8YFLogxK

U2 - 10.1093/clinchem/23.8.1492

DO - 10.1093/clinchem/23.8.1492

M3 - Article

C2 - 872409

AN - SCOPUS:0017642057

VL - 23

SP - 1492

EP - 1498

JO - Scientific Computing and Instrumentation

JF - Scientific Computing and Instrumentation

SN - 1078-8956

IS - 8

ER -