Development of instrumentation and fluorochromes for automated multiparameter analysis of cells

R. C. Leif; R. A. Thomas; T. A. Yopp; B. D. Watson; V. R. Guarino; D. H. Hindman; N. Lefkove; L. M. Vallarino

doi:10.1093/clinchem/23.8.1492

Development of instrumentation and fluorochromes for automated multiparameter analysis of cells

R. C. Leif, R. A. Thomas, T. A. Yopp, B. D. Watson, V. R. Guarino, D. H. Hindman, N. Lefkove, L. M. Vallarino

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.

Original language	English (US)
Pages (from-to)	1492-1498
Number of pages	7
Journal	Unknown Journal
Volume	23
Issue number	8
DOIs	https://doi.org/10.1093/clinchem/23.8.1492
State	Published - 1977
Externally published	Yes

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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abstract = "We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.",

author = "Leif, {R. C.} and Thomas, {R. A.} and Yopp, {T. A.} and Watson, {B. D.} and Guarino, {V. R.} and Hindman, {D. H.} and N. Lefkove and Vallarino, {L. M.}",

year = "1977",

doi = "10.1093/clinchem/23.8.1492",

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AU - Leif, R. C.

AU - Thomas, R. A.

AU - Yopp, T. A.

AU - Watson, B. D.

AU - Guarino, V. R.

AU - Hindman, D. H.

AU - Lefkove, N.

AU - Vallarino, L. M.

PY - 1977

Y1 - 1977

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AB - We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.

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Development of instrumentation and fluorochromes for automated multiparameter analysis of cells

Abstract

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