Development of instrumentation and fluorochromes for automated multiparameter analysis of cells

R. C. Leif, R. A. Thomas, T. A. Yopp, B. D. Watson, V. R. Guarino, D. H. Hindman, N. Lefkove, L. M. Vallarino

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We have developed and interfaced to a computer an automated instrument (the AMAC III) which is designed to observe simultaneously several physical parameters of cells. Typical parameters include electronic cell volume (Coulter effect), RF amplitude (opacity), multiwavelength fluorescence of cytological stains, and cell light scattering. The use of a new ultraviolet laser combined with a holographic grating spectrograph promises to increase the number of fluorescing species that can be detected simultaneously. This number can be further increased by use of special rare earth based fluorochromes, that emit well defined, spectrally distinct peaks.

Original languageEnglish
Pages (from-to)1492-1498
Number of pages7
JournalClinical Chemistry
Volume23
Issue number8
StatePublished - Dec 1 1977

Fingerprint

Holographic gratings
Ultraviolet lasers
Spectrographs
Opacity
Fluorescent Dyes
Light scattering
Rare earths
Coloring Agents
Fluorescence
Cell Size
Lasers
Light

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Leif, R. C., Thomas, R. A., Yopp, T. A., Watson, B. D., Guarino, V. R., Hindman, D. H., ... Vallarino, L. M. (1977). Development of instrumentation and fluorochromes for automated multiparameter analysis of cells. Clinical Chemistry, 23(8), 1492-1498.

Development of instrumentation and fluorochromes for automated multiparameter analysis of cells. / Leif, R. C.; Thomas, R. A.; Yopp, T. A.; Watson, B. D.; Guarino, V. R.; Hindman, D. H.; Lefkove, N.; Vallarino, L. M.

In: Clinical Chemistry, Vol. 23, No. 8, 01.12.1977, p. 1492-1498.

Research output: Contribution to journalArticle

Leif, RC, Thomas, RA, Yopp, TA, Watson, BD, Guarino, VR, Hindman, DH, Lefkove, N & Vallarino, LM 1977, 'Development of instrumentation and fluorochromes for automated multiparameter analysis of cells', Clinical Chemistry, vol. 23, no. 8, pp. 1492-1498.
Leif RC, Thomas RA, Yopp TA, Watson BD, Guarino VR, Hindman DH et al. Development of instrumentation and fluorochromes for automated multiparameter analysis of cells. Clinical Chemistry. 1977 Dec 1;23(8):1492-1498.
Leif, R. C. ; Thomas, R. A. ; Yopp, T. A. ; Watson, B. D. ; Guarino, V. R. ; Hindman, D. H. ; Lefkove, N. ; Vallarino, L. M. / Development of instrumentation and fluorochromes for automated multiparameter analysis of cells. In: Clinical Chemistry. 1977 ; Vol. 23, No. 8. pp. 1492-1498.
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