Development of compensatory thermogenesis in response to overfeeding in hypothyroid rats

Cyntia Curcio, Agatha M. Lopes, Miriam O. Ribeiro, Ophelis A. Francoso, Suzy D. Carvalho, Fábio B. Lima, José E. Bicudo, Antonio C. Bianco

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Intact or surgically thyroidectomized (Tx) adult male Wistar rats, weighing 150-200 g, were fed a standard chow diet (~1.8 Cal/g) or a high calorie (~3.8 Cal/g) diet (cafeteria diet) for up to 30 days. Daily energy intake was about 5-fold higher in the rats fed the cafeteria diet regardless of their thyroid status. The cafeteria diet caused the retroperitoneal white fat pad to increase by approximately 2-fold, the volume of isolated white adipocytes to increase by 2-fold, and the total body fat to increase by a factor of approximately 3, again regardless of thyroid status. It also increased basal metabolic rate by about 20% in intact rats and by about 50% in Tx rats. The brown fat thermal response to norepinephrine (NE) infusion was approximately 2-fold increased in the intact rats fed the cafeteria diet. However, in the Tx rats, the brown fat thermal response to NE was blunted regardless of the dietary regimen adopted. In both intact and Tx rats, the cafeteria diet increased total brown fat mitochondria, uncoupling protein percentage, and total brown fat uncoupling protein by about 3-, 2-, and 5-fold, respectively. Serum leptin levels also increased approximately 4-fold in intact rats fed the cafeteria diet. However, in Tx rats, leptin levels did not change significantly during overfeeding. In conclusion, hypothyroidism caused the brown fat to become unresponsive to NE, even after 1 month on the cafeteria diet. However, these rats were able to increase basal metabolic rate and, as assessed by several different parameters, did not gain fat beyond that observed in intact controls kept on a similar overfeeding schedule.

Original languageEnglish
Pages (from-to)3438-3443
Number of pages6
JournalEndocrinology
Volume140
Issue number8
StatePublished - Dec 1 1999

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Thermogenesis
Diet
Brown Adipose Tissue
Basal Metabolism
Norepinephrine
Leptin
Adipose Tissue
Thyroid Gland
Hot Temperature
White Adipocytes
White Adipose Tissue
Intra-Abdominal Fat
Hypothyroidism
Energy Intake
Wistar Rats
Appointments and Schedules
Mitochondria
Fats

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Curcio, C., Lopes, A. M., Ribeiro, M. O., Francoso, O. A., Carvalho, S. D., Lima, F. B., ... Bianco, A. C. (1999). Development of compensatory thermogenesis in response to overfeeding in hypothyroid rats. Endocrinology, 140(8), 3438-3443.

Development of compensatory thermogenesis in response to overfeeding in hypothyroid rats. / Curcio, Cyntia; Lopes, Agatha M.; Ribeiro, Miriam O.; Francoso, Ophelis A.; Carvalho, Suzy D.; Lima, Fábio B.; Bicudo, José E.; Bianco, Antonio C.

In: Endocrinology, Vol. 140, No. 8, 01.12.1999, p. 3438-3443.

Research output: Contribution to journalArticle

Curcio, C, Lopes, AM, Ribeiro, MO, Francoso, OA, Carvalho, SD, Lima, FB, Bicudo, JE & Bianco, AC 1999, 'Development of compensatory thermogenesis in response to overfeeding in hypothyroid rats', Endocrinology, vol. 140, no. 8, pp. 3438-3443.
Curcio C, Lopes AM, Ribeiro MO, Francoso OA, Carvalho SD, Lima FB et al. Development of compensatory thermogenesis in response to overfeeding in hypothyroid rats. Endocrinology. 1999 Dec 1;140(8):3438-3443.
Curcio, Cyntia ; Lopes, Agatha M. ; Ribeiro, Miriam O. ; Francoso, Ophelis A. ; Carvalho, Suzy D. ; Lima, Fábio B. ; Bicudo, José E. ; Bianco, Antonio C. / Development of compensatory thermogenesis in response to overfeeding in hypothyroid rats. In: Endocrinology. 1999 ; Vol. 140, No. 8. pp. 3438-3443.
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