In the development of antimicrobial agents, the use of both in-vitro and in-vivo models is essential for successful outcomes. In-vitro tests are necessary to help determine initial dose-response data as well as to evaluate potential susceptibility and/or resistance of specific pathogens. The employment of appropriate in-vivo animal testing is vital prior to clinical evaluations, since factors, such as wound fluid, growth factors, etc., need to be taken into account. When performing both of these types of evaluations (in vitro and in vivo), one also needs to be aware of which bacteria form is being studied, e.g., free floating (planktonic) or biofilm. This is especially critical, since bacteria living in a biofilm have been shown to be more resistant to therapies. The translation of in-vitro and in-vivo studies from the laboratory into the clinic is necessary to obtain successful antimicrobial therapeutics.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Nov 1 2004|
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