Development of a set of simple bacterial biosensors for quantitative and rapid measurements of arsenite and arsenate in potable water

Judith Stocker, Denisa Balluch, Monika Gsell, Hauke Harms, Jessika Feliciano, Sylvia Daunert, Khurseed A. Malik, Jan Roelof Van der Meer

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

Testing for arsenic pollution is commonly performed with chemical test kits of unsatisfying accuracy. Bacterial biosensors are an interesting alternative as they are easily produced, simple, and highly accurate devices. Here, we describe the development of a set of bacterial biosensors based on a nonpathogenic laboratory strain of Escherichia coli, the natural resistance mechanism of E. coli against arsenite and arsenate, and three reporter proteins: bacterial luciferase, β-galactosidase and Green Fluorescent Protein (GFP). The biosensors were genetically optimized to reduce background expression in the absence of arsenic. In calibration experiments with the biosensors and arsenite-amended potable water, arsenite concentrations at 4 μg of As/L (0. 05 μM) were routinely and accurately measured. The currently most quantitative system expressed the bacteria luciferase as reporter protein, responding proportional with a concentration range between 8 and 80 μg of As/L. Sensor cells could be stored as frozen batches, resuspended in plain media, and exposed to the aqueous test sample, and light emission was measured after 30-min incubation. Field testing for arsenite was achieved with a system that contained β-galactosidase, producing a visible blue color at arsenite concentrations above 8 μg/L. For this sensor, a protocol was developed in which the sensor cells were dried on a paper strip and placed in the aqueous test solution for 30 min after which time color development was allowed to take place. The GFP sensor showed good potential for continuous rather than end point measurements. In all cases, growth of the biosensors and production of the strip test was achieved by very simple means with common growth media, and quality control of the sensors was performed by isolating the respective plasmids with the genetic constructs according to simple standard genetic technologies. Therefore, the biosensor cells and protocols may offer a realistic alternative for measuring arsenic contamination in potable water.

Original languageEnglish
Pages (from-to)4743-4750
Number of pages8
JournalEnvironmental Science and Technology
Volume37
Issue number20
DOIs
StatePublished - Oct 15 2003
Externally publishedYes

Fingerprint

arsenite
arsenate
Biosensors
Potable water
Drinking Water
drinking water
sensor
Arsenic
arsenic
Galactosidases
protein
Sensors
Green Fluorescent Proteins
Proteins
Escherichia coli
Bacterial Luciferases
Color
plasmid
quality control
Light emission

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Development of a set of simple bacterial biosensors for quantitative and rapid measurements of arsenite and arsenate in potable water. / Stocker, Judith; Balluch, Denisa; Gsell, Monika; Harms, Hauke; Feliciano, Jessika; Daunert, Sylvia; Malik, Khurseed A.; Van der Meer, Jan Roelof.

In: Environmental Science and Technology, Vol. 37, No. 20, 15.10.2003, p. 4743-4750.

Research output: Contribution to journalArticle

Stocker, Judith ; Balluch, Denisa ; Gsell, Monika ; Harms, Hauke ; Feliciano, Jessika ; Daunert, Sylvia ; Malik, Khurseed A. ; Van der Meer, Jan Roelof. / Development of a set of simple bacterial biosensors for quantitative and rapid measurements of arsenite and arsenate in potable water. In: Environmental Science and Technology. 2003 ; Vol. 37, No. 20. pp. 4743-4750.
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