Development of a new bioartificial liver using a porcine autologous biomatrix as hepatocyte support

Giovanni Ambrosino, Sergio Varotto, M. M.Stefano Basso, Daniele Galavotti, Attilio Cecchetto, Paolo Carraro, Agostino Naso, Giustina De Silvestro, Mario Plebani, Gianpiero Giron, Giovanni Abatangelo, Daniele Donato, Adriano Cestrone, Luigi Marrelli, Marcella Trombetta, Vincenzo Lorenzelli, Antonio Picardi, Alessandra Colantoni, David Van Thiel, Camillo RicordiFrancesco Davide D'Amico

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Long-term maintenance of hepatocyte viability and differentiated function expression is crucial for bioartificial liver support. The maintenance of hepatocyte function in a bioreactor is still a problem. A major advance was the recognition that hepatocytes in attachment cultures can maintain their differentiation longer. To restore hepatocyte polarity and prolong their function, we developed a new bioreactor with a crossflow geometry configuration and an original hepatocyte extracellular autologous biomatrix (Porcine Bio-Matrix) support. To test this new bioreactor, we compared it with a standard bioartificial liver cartridge in a suitable surgical model of acute liver failure in pigs. In our model, we performed a total hepatectomy, followed by partial liver transplantation after an 18 hour anhepatic phase. The results showed that the bioreactor containing the biomatrix was able to bridge the animal to transplantation and to sustain the transplanted liver until all function recovered (80% of animals survived, p = 0.0027). No animal survived more than 24 hours after liver transplantation in the group treated with the traditional bioartificial liver, whereas hepatocyte viability on the Porcine Bio-Matrix was 65% after 12 hours of treatment. The results suggest that our biomatrix is a suitable cell support and guarantees long-term maintenance of metabolic activity of hepatocytes. Further studies are needed, but the results obtained with this new three-dimensional bioreactor are promising, and its potential is attractive.

Original languageEnglish (US)
Pages (from-to)592-597
Number of pages6
JournalASAIO Journal
Volume48
Issue number6
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Ambrosino, G., Varotto, S., Basso, M. M. S., Galavotti, D., Cecchetto, A., Carraro, P., Naso, A., De Silvestro, G., Plebani, M., Giron, G., Abatangelo, G., Donato, D., Cestrone, A., Marrelli, L., Trombetta, M., Lorenzelli, V., Picardi, A., Colantoni, A., Van Thiel, D., ... D'Amico, F. D. (2002). Development of a new bioartificial liver using a porcine autologous biomatrix as hepatocyte support. ASAIO Journal, 48(6), 592-597. https://doi.org/10.1097/00002480-200211000-00004