Development of a method to test anti-LH-RH activity in rats

J. A. Vilchez-Martinez, A. V. Schally, L. Debeljuk, D. H. Coy, E. Coy, A. Arimura, N. Yanaihara

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A method for testing anti LH RH activity was devised. The substance to be investigated was infused for 2 hr i.c. in ovariectomized, estrogen and progesterone pretreated rats, followed by an acute injection of synthetic LH RH into the jugular vein. The serum LH concentration was investigated before and 20 min after the injection of LH RH. Among all the peptides investigated, des His 2 LH RH was the only one that did not modify the response to LH RH. The infusion with (pyro)Glu His Trp NH 2, Leu 3 LH RH, and des Trp 3 des Gly 10 LH RH only partially inhibited the response to LH RH, whereas comparatively lower doses of des His 2 des Gly 10 LH RH ethylamide significantly blocked the LH release after the administration of LH RH.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalNeuroendocrinology
Volume14
Issue number2
DOIs
StatePublished - Jan 1 1974
Externally publishedYes

Keywords

  • Anti-LH-RH methods
  • LH release
  • LH-releasing hormone
  • Peptide antagonists

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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    Vilchez-Martinez, J. A., Schally, A. V., Debeljuk, L., Coy, D. H., Coy, E., Arimura, A., & Yanaihara, N. (1974). Development of a method to test anti-LH-RH activity in rats. Neuroendocrinology, 14(2), 121-128. https://doi.org/10.1159/000122252