Development of a method to test anti LH RH activity in rats

J. A. Vilchez-Martinez, Andrew V Schally, L. Debeljuk, D. H. Coy, E. Coy, A. Arimura, N. Yanaihara

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A method for testing anti LH RH activity was devised. The substance to be investigated was infused for 2 hr i.c. in ovariectomized, estrogen and progesterone pretreated rats, followed by an acute injection of synthetic LH RH into the jugular vein. The serum LH concentration was investigated before and 20 min after the injection of LH RH. Among all the peptides investigated, des His 2 LH RH was the only one that did not modify the response to LH RH. The infusion with (pyro)Glu His Trp NH 2, Leu 3 LH RH, and des Trp 3 des Gly 10 LH RH only partially inhibited the response to LH RH, whereas comparatively lower doses of des His 2 des Gly 10 LH RH ethylamide significantly blocked the LH release after the administration of LH RH.

Original languageEnglish
Pages (from-to)121-128
Number of pages8
JournalNeuroendocrinology
Volume14
Issue number2
StatePublished - Jan 1 1974
Externally publishedYes

Fingerprint

Gonadotropin-Releasing Hormone
Injections
Jugular Veins
Progesterone
Estrogens
Peptides
Serum

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Vilchez-Martinez, J. A., Schally, A. V., Debeljuk, L., Coy, D. H., Coy, E., Arimura, A., & Yanaihara, N. (1974). Development of a method to test anti LH RH activity in rats. Neuroendocrinology, 14(2), 121-128.

Development of a method to test anti LH RH activity in rats. / Vilchez-Martinez, J. A.; Schally, Andrew V; Debeljuk, L.; Coy, D. H.; Coy, E.; Arimura, A.; Yanaihara, N.

In: Neuroendocrinology, Vol. 14, No. 2, 01.01.1974, p. 121-128.

Research output: Contribution to journalArticle

Vilchez-Martinez, JA, Schally, AV, Debeljuk, L, Coy, DH, Coy, E, Arimura, A & Yanaihara, N 1974, 'Development of a method to test anti LH RH activity in rats', Neuroendocrinology, vol. 14, no. 2, pp. 121-128.
Vilchez-Martinez JA, Schally AV, Debeljuk L, Coy DH, Coy E, Arimura A et al. Development of a method to test anti LH RH activity in rats. Neuroendocrinology. 1974 Jan 1;14(2):121-128.
Vilchez-Martinez, J. A. ; Schally, Andrew V ; Debeljuk, L. ; Coy, D. H. ; Coy, E. ; Arimura, A. ; Yanaihara, N. / Development of a method to test anti LH RH activity in rats. In: Neuroendocrinology. 1974 ; Vol. 14, No. 2. pp. 121-128.
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