Development and Validation of a Small Animal Immobilizer and Positioning System for the Study of Delivery of Intracranial and Extracranial Radiotherapy Using the Gamma Knife System

Musaddiq J. Awan, Jennifer Dorth, Arvind Mani, Haksoo Kim, Yiran Zheng, Mazen Mislmani, Scott Welford, Jiankui Yuan, Barry W. Wessels, Simon S. Lo, John Letterio, Mitchell Machtay, Andrew Sloan, Jason W. Sohn

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The purpose of this research is to establish a process of irradiating mice using the Gamma Knife as a versatile system for small animal irradiation and to validate accurate intracranial and extracranial dose delivery using this system. A stereotactic immobilization device was developed for small animals for the Gamma Knife head frame allowing for isocentric dose delivery. Intercranial positional reproducibility of a reference point from a primary reference animal was verified on an additional mouse. Extracranial positional reproducibility of the mouse aorta was verified using 3 mice. Accurate dose delivery was validated using film and thermoluminescent dosimeter measurements with a solid water phantom. Gamma Knife plans were developed to irradiate intracranial and extracranial targets. Mice were irradiated validating successful targeted radiation dose delivery. Intramouse positional variability of the right mandible reference point across 10 micro-computed tomography scans was 0.65 ± 0.48 mm. Intermouse positional reproducibility across 2 mice at the same reference point was 0.76 ± 0.46 mm. The accuracy of dose delivery was 0.67 ± 0.29 mm and 1.01 ± 0.43 mm in the coronal and sagittal planes, respectively. The planned dose delivered to a mouse phantom was 2 Gy at the 50% isodose with a measured thermoluminescent dosimeter dose of 2.9 ± 0.3 Gy. The phosphorylated form of member X of histone family H2A (γH2AX) staining of irradiated mouse brain and mouse aorta demonstrated adjacent tissue sparing. In conclusion, our system for preclinical studies of small animal irradiation using the Gamma Knife is able to accurately deliver intracranial and extracranial targeted focal radiation allowing for preclinical experiments studying focal radiation.

Original languageEnglish (US)
Pages (from-to)203-210
Number of pages8
JournalTechnology in Cancer Research and Treatment
Volume16
Issue number2
DOIs
StatePublished - Apr 1 2017
Externally publishedYes

Fingerprint

Radiotherapy
Radiation
Aorta
Mandible
Immobilization
Histones
Head
Tomography
Staining and Labeling
Equipment and Supplies
Water
Brain
Research

Keywords

  • conformal radiotherapy
  • preclinical models
  • radiation biology
  • radiation physics
  • small animal irradiation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Development and Validation of a Small Animal Immobilizer and Positioning System for the Study of Delivery of Intracranial and Extracranial Radiotherapy Using the Gamma Knife System. / Awan, Musaddiq J.; Dorth, Jennifer; Mani, Arvind; Kim, Haksoo; Zheng, Yiran; Mislmani, Mazen; Welford, Scott; Yuan, Jiankui; Wessels, Barry W.; Lo, Simon S.; Letterio, John; Machtay, Mitchell; Sloan, Andrew; Sohn, Jason W.

In: Technology in Cancer Research and Treatment, Vol. 16, No. 2, 01.04.2017, p. 203-210.

Research output: Contribution to journalArticle

Awan, Musaddiq J. ; Dorth, Jennifer ; Mani, Arvind ; Kim, Haksoo ; Zheng, Yiran ; Mislmani, Mazen ; Welford, Scott ; Yuan, Jiankui ; Wessels, Barry W. ; Lo, Simon S. ; Letterio, John ; Machtay, Mitchell ; Sloan, Andrew ; Sohn, Jason W. / Development and Validation of a Small Animal Immobilizer and Positioning System for the Study of Delivery of Intracranial and Extracranial Radiotherapy Using the Gamma Knife System. In: Technology in Cancer Research and Treatment. 2017 ; Vol. 16, No. 2. pp. 203-210.
@article{25557559087e40028994cdbc542b7c04,
title = "Development and Validation of a Small Animal Immobilizer and Positioning System for the Study of Delivery of Intracranial and Extracranial Radiotherapy Using the Gamma Knife System",
abstract = "The purpose of this research is to establish a process of irradiating mice using the Gamma Knife as a versatile system for small animal irradiation and to validate accurate intracranial and extracranial dose delivery using this system. A stereotactic immobilization device was developed for small animals for the Gamma Knife head frame allowing for isocentric dose delivery. Intercranial positional reproducibility of a reference point from a primary reference animal was verified on an additional mouse. Extracranial positional reproducibility of the mouse aorta was verified using 3 mice. Accurate dose delivery was validated using film and thermoluminescent dosimeter measurements with a solid water phantom. Gamma Knife plans were developed to irradiate intracranial and extracranial targets. Mice were irradiated validating successful targeted radiation dose delivery. Intramouse positional variability of the right mandible reference point across 10 micro-computed tomography scans was 0.65 ± 0.48 mm. Intermouse positional reproducibility across 2 mice at the same reference point was 0.76 ± 0.46 mm. The accuracy of dose delivery was 0.67 ± 0.29 mm and 1.01 ± 0.43 mm in the coronal and sagittal planes, respectively. The planned dose delivered to a mouse phantom was 2 Gy at the 50{\%} isodose with a measured thermoluminescent dosimeter dose of 2.9 ± 0.3 Gy. The phosphorylated form of member X of histone family H2A (γH2AX) staining of irradiated mouse brain and mouse aorta demonstrated adjacent tissue sparing. In conclusion, our system for preclinical studies of small animal irradiation using the Gamma Knife is able to accurately deliver intracranial and extracranial targeted focal radiation allowing for preclinical experiments studying focal radiation.",
keywords = "conformal radiotherapy, preclinical models, radiation biology, radiation physics, small animal irradiation",
author = "Awan, {Musaddiq J.} and Jennifer Dorth and Arvind Mani and Haksoo Kim and Yiran Zheng and Mazen Mislmani and Scott Welford and Jiankui Yuan and Wessels, {Barry W.} and Lo, {Simon S.} and John Letterio and Mitchell Machtay and Andrew Sloan and Sohn, {Jason W.}",
year = "2017",
month = "4",
day = "1",
doi = "10.1177/1533034616658394",
language = "English (US)",
volume = "16",
pages = "203--210",
journal = "Technology in Cancer Research and Treatment",
issn = "1533-0346",
publisher = "Adenine Press",
number = "2",

}

TY - JOUR

T1 - Development and Validation of a Small Animal Immobilizer and Positioning System for the Study of Delivery of Intracranial and Extracranial Radiotherapy Using the Gamma Knife System

AU - Awan, Musaddiq J.

AU - Dorth, Jennifer

AU - Mani, Arvind

AU - Kim, Haksoo

AU - Zheng, Yiran

AU - Mislmani, Mazen

AU - Welford, Scott

AU - Yuan, Jiankui

AU - Wessels, Barry W.

AU - Lo, Simon S.

AU - Letterio, John

AU - Machtay, Mitchell

AU - Sloan, Andrew

AU - Sohn, Jason W.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - The purpose of this research is to establish a process of irradiating mice using the Gamma Knife as a versatile system for small animal irradiation and to validate accurate intracranial and extracranial dose delivery using this system. A stereotactic immobilization device was developed for small animals for the Gamma Knife head frame allowing for isocentric dose delivery. Intercranial positional reproducibility of a reference point from a primary reference animal was verified on an additional mouse. Extracranial positional reproducibility of the mouse aorta was verified using 3 mice. Accurate dose delivery was validated using film and thermoluminescent dosimeter measurements with a solid water phantom. Gamma Knife plans were developed to irradiate intracranial and extracranial targets. Mice were irradiated validating successful targeted radiation dose delivery. Intramouse positional variability of the right mandible reference point across 10 micro-computed tomography scans was 0.65 ± 0.48 mm. Intermouse positional reproducibility across 2 mice at the same reference point was 0.76 ± 0.46 mm. The accuracy of dose delivery was 0.67 ± 0.29 mm and 1.01 ± 0.43 mm in the coronal and sagittal planes, respectively. The planned dose delivered to a mouse phantom was 2 Gy at the 50% isodose with a measured thermoluminescent dosimeter dose of 2.9 ± 0.3 Gy. The phosphorylated form of member X of histone family H2A (γH2AX) staining of irradiated mouse brain and mouse aorta demonstrated adjacent tissue sparing. In conclusion, our system for preclinical studies of small animal irradiation using the Gamma Knife is able to accurately deliver intracranial and extracranial targeted focal radiation allowing for preclinical experiments studying focal radiation.

AB - The purpose of this research is to establish a process of irradiating mice using the Gamma Knife as a versatile system for small animal irradiation and to validate accurate intracranial and extracranial dose delivery using this system. A stereotactic immobilization device was developed for small animals for the Gamma Knife head frame allowing for isocentric dose delivery. Intercranial positional reproducibility of a reference point from a primary reference animal was verified on an additional mouse. Extracranial positional reproducibility of the mouse aorta was verified using 3 mice. Accurate dose delivery was validated using film and thermoluminescent dosimeter measurements with a solid water phantom. Gamma Knife plans were developed to irradiate intracranial and extracranial targets. Mice were irradiated validating successful targeted radiation dose delivery. Intramouse positional variability of the right mandible reference point across 10 micro-computed tomography scans was 0.65 ± 0.48 mm. Intermouse positional reproducibility across 2 mice at the same reference point was 0.76 ± 0.46 mm. The accuracy of dose delivery was 0.67 ± 0.29 mm and 1.01 ± 0.43 mm in the coronal and sagittal planes, respectively. The planned dose delivered to a mouse phantom was 2 Gy at the 50% isodose with a measured thermoluminescent dosimeter dose of 2.9 ± 0.3 Gy. The phosphorylated form of member X of histone family H2A (γH2AX) staining of irradiated mouse brain and mouse aorta demonstrated adjacent tissue sparing. In conclusion, our system for preclinical studies of small animal irradiation using the Gamma Knife is able to accurately deliver intracranial and extracranial targeted focal radiation allowing for preclinical experiments studying focal radiation.

KW - conformal radiotherapy

KW - preclinical models

KW - radiation biology

KW - radiation physics

KW - small animal irradiation

UR - http://www.scopus.com/inward/record.url?scp=85015068895&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85015068895&partnerID=8YFLogxK

U2 - 10.1177/1533034616658394

DO - 10.1177/1533034616658394

M3 - Article

C2 - 27444980

AN - SCOPUS:85015068895

VL - 16

SP - 203

EP - 210

JO - Technology in Cancer Research and Treatment

JF - Technology in Cancer Research and Treatment

SN - 1533-0346

IS - 2

ER -