Determination of the route of medium-chain and long-chain fatty acid absorption by direct measurement in the rat

David L. Sigalet, Gerrit B. Winkelaar, Lesley J Smith

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Medium-chain triglycerides are used for the treatment of malabsorptive states. We measured directly the absorption of medium- and long-chain fatty acids via the mesenteric lymphatics and portal vein in normal animals. These results may be useful in guiding therapy for short- bowel syndrome. Methods: Under anesthesia, male Lewis rats (n = 6) underwent placement of jugular and portal venous lines, mesenteric lymphatic duct cannula, and a duodenal tube. After recovery, a 0.3% lauric acid (C12:0) and 0.37% palmitic acid (C16:0) solution solublized with 3.25 mmol/L lecithin and 23.75 mmol/L taurocholic acid in phosphate-buffered saline was infused at a rate of 3 mL/h via the duodenal tube. After stabilization of lymphatic flow (6 hours), a pulse of radiolabeled lauric and palmitic acid was given via the duodenal tube; absorption was measured by collection of lymphatic fluid and sampling of the portal and jugular venous blood for 4 hours. Results: The amount of acid recovered in the lymphatics was 51% ± 6% (mean ± SD) for lauric and 59% ± 6% for palmitic. For both fatty acids, <1% in total was recovered from the portal vein during the 4-hour postbolus period; thereafter, levels in the blood were constant and very low. At necropsy, the majority of the remaining label was found in the intestine. Conclusions: These results show no evidence for the preferential absorption of medium- chain fatty acids directly via the portal vein in this model. We suggest that further studies be done to measure directly portal vein absorption of the medium-chain fatty acids.

Original languageEnglish
Pages (from-to)275-278
Number of pages4
JournalJournal of Parenteral and Enteral Nutrition
Volume21
Issue number5
StatePublished - Sep 1 1997
Externally publishedYes

Fingerprint

lauric acid
portal vein
long chain fatty acids
Portal Vein
medium chain fatty acids
Fatty Acids
dodecanoic acid
rats
palmitic acid
neck
Neck
taurocholic acid
Short Bowel Syndrome
Mesenteric Veins
medium chain triacylglycerols
Taurocholic Acid
Palmitic Acid
Lecithins
blood
cannulas

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Determination of the route of medium-chain and long-chain fatty acid absorption by direct measurement in the rat. / Sigalet, David L.; Winkelaar, Gerrit B.; Smith, Lesley J.

In: Journal of Parenteral and Enteral Nutrition, Vol. 21, No. 5, 01.09.1997, p. 275-278.

Research output: Contribution to journalArticle

Sigalet, David L. ; Winkelaar, Gerrit B. ; Smith, Lesley J. / Determination of the route of medium-chain and long-chain fatty acid absorption by direct measurement in the rat. In: Journal of Parenteral and Enteral Nutrition. 1997 ; Vol. 21, No. 5. pp. 275-278.
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