Abstract
The reduction with sodium borohydride-t of a series of maltose oligosaccharides yields products whose residual radioactivity (after deduction of a blank) has a linear dependence on degree of polymerisation. The procedure has been extended to measure the average degree of polymerisation (or alternatively Mn) of hydrolysed amyloses. For this purpose, it is necessary to use a washing procedure to remove a radioactive impurity in the sodium borohydride. The method should be applicable to measurement of number-average molecular weights of any polysaccharide which has a reducing end-group.
| Original language | English |
|---|---|
| Pages (from-to) | 185-191 |
| Number of pages | 7 |
| Journal | Carbohydrate Research |
| Volume | 27 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1973 |
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ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Organic Chemistry
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Determination of the number-average molecular weight of polysaccharides by end-group reduction with borohydride-t. / Richards, G. N.; Whelan, William J.
In: Carbohydrate Research, Vol. 27, No. 1, 01.01.1973, p. 185-191.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of the number-average molecular weight of polysaccharides by end-group reduction with borohydride-t
AU - Richards, G. N.
AU - Whelan, William J.
PY - 1973/1/1
Y1 - 1973/1/1
N2 - The reduction with sodium borohydride-t of a series of maltose oligosaccharides yields products whose residual radioactivity (after deduction of a blank) has a linear dependence on degree of polymerisation. The procedure has been extended to measure the average degree of polymerisation (or alternatively Mn) of hydrolysed amyloses. For this purpose, it is necessary to use a washing procedure to remove a radioactive impurity in the sodium borohydride. The method should be applicable to measurement of number-average molecular weights of any polysaccharide which has a reducing end-group.
AB - The reduction with sodium borohydride-t of a series of maltose oligosaccharides yields products whose residual radioactivity (after deduction of a blank) has a linear dependence on degree of polymerisation. The procedure has been extended to measure the average degree of polymerisation (or alternatively Mn) of hydrolysed amyloses. For this purpose, it is necessary to use a washing procedure to remove a radioactive impurity in the sodium borohydride. The method should be applicable to measurement of number-average molecular weights of any polysaccharide which has a reducing end-group.
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UR - http://www.scopus.com/inward/citedby.url?scp=0015591678&partnerID=8YFLogxK
U2 - 10.1016/S0008-6215(00)82438-6
DO - 10.1016/S0008-6215(00)82438-6
M3 - Article
VL - 27
SP - 185
EP - 191
JO - Carbohydrate Research
JF - Carbohydrate Research
SN - 0008-6215
IS - 1
ER -