Determination of the amino acid sequence of troponin C from rabbit skeletal muscle

J. H. Collins, M. L. Greaser, J. D. Potter, M. J. Horn

Research output: Contribution to journalArticle

Abstract

The complete amino acid sequence of rabbit skeletal muscle troponin C (TnC) was determined from studies on overlapping peptides isolated from cyanogen bromide and tryptic digests. TnC was found to be a single polypeptide chain of 159 amino acid residues, including 2 residues of tyrosine and 1 each of cysteine, histidine, and proline. The amino end is acetylated, the calculated net charge at pH 7.0 is -29, and the calculated molecular weight is 17,965. There was no evidence for heterogeneity in the sequence. The previously proposed four apparent Ca2+ binding sites are located at residues 27 to 38, 63 to 74, 103 to 114, and 139 to 150.

Original languageEnglish
Pages (from-to)6356-6362
Number of pages7
JournalJournal of Biological Chemistry
Volume252
Issue number18
StatePublished - Dec 1 1977
Externally publishedYes

Fingerprint

Troponin C
Protein Sequence Analysis
Muscle
Skeletal Muscle
Rabbits
Amino Acids
Cyanogen Bromide
Peptides
Histidine
Proline
Cysteine
Tyrosine
Amino Acid Sequence
Molecular Weight
Molecular weight
Binding Sites
2-tyrosine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Collins, J. H., Greaser, M. L., Potter, J. D., & Horn, M. J. (1977). Determination of the amino acid sequence of troponin C from rabbit skeletal muscle. Journal of Biological Chemistry, 252(18), 6356-6362.

Determination of the amino acid sequence of troponin C from rabbit skeletal muscle. / Collins, J. H.; Greaser, M. L.; Potter, J. D.; Horn, M. J.

In: Journal of Biological Chemistry, Vol. 252, No. 18, 01.12.1977, p. 6356-6362.

Research output: Contribution to journalArticle

Collins, JH, Greaser, ML, Potter, JD & Horn, MJ 1977, 'Determination of the amino acid sequence of troponin C from rabbit skeletal muscle', Journal of Biological Chemistry, vol. 252, no. 18, pp. 6356-6362.
Collins JH, Greaser ML, Potter JD, Horn MJ. Determination of the amino acid sequence of troponin C from rabbit skeletal muscle. Journal of Biological Chemistry. 1977 Dec 1;252(18):6356-6362.
Collins, J. H. ; Greaser, M. L. ; Potter, J. D. ; Horn, M. J. / Determination of the amino acid sequence of troponin C from rabbit skeletal muscle. In: Journal of Biological Chemistry. 1977 ; Vol. 252, No. 18. pp. 6356-6362.
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