Determination of 1-(2-chloroethyl)-3-(2,6-dioxo-3-piperidyl)-1-nitrosourea in plasma by negative chemical ionization mass spectrometry

R. G. Smith, L. K. Cheung, Lynn G Feun, T. L. Loo

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5 Scopus citations


A sensitive and specific method for the quantitative determination of a new antitumor agent, 1-(2-chloroethyl)-3-(2,6-dioxo-3-piperidyl)-1-nitrosourea, (PCNU) has been developed for the analysis of plasma. This assay involves extraction of the plasma sample, separation of the drug by thin-layer chromatography and mass spectrometric detection of the negative ions derived from the unchanged drug and its 2H4-labeled analog. Ion current profile peaks are obtained when drug samples are introduced into the heated source using a desorption chemical ionization probe. Detection limits are below 1.0 ng ml-1 of plasma. This method has been applied to determine the in vitro rate of decomposition of PCNU in plasma and the plasma clearance of this drug from a cancer patient.

Original languageEnglish
Pages (from-to)404-407
Number of pages4
JournalBiomedical Mass Spectrometry
Issue number7
StatePublished - Dec 1 1983
Externally publishedYes


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pollution

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