Detection of retroviral superantigen and products of the envelope gene from endogenous mouse mammary tumor virus in B cells from BALB/c mice

Mayra Lopez-Cepero, Yang Wang, Iafa Keydar, Carolyn Brandt-Carlson, Janet S. Butel, Diana M Lopez

Research output: Contribution to journalArticle

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Abstract

Minor lymphocyte-stimulating antigens and other superantigens have been shown to be encoded by the 3' long terminal repeat (LTR) open reading frame (ORF) of the endogenous and exogenous mammary tumor viruses. We have previously reported the presence of an antigen(s) related to mouse mammary tumor virus (MMTV) env products in splenic B cells of BALB/c mice. By Western blots an MMTV related molecule of 68 kDa was detected in splenic preparations of B lymphocytes, but not in T cells. Antibodies against the MMTV envelope proteins gp52 and gp36, obtained by elution after binding to nitrocellulose in the presence of purified MMTV, reacted in Western blots with a 68-kDa protein present in B cells, indicating that this molecule is related to both MMTV envelope proteins. Using antibody against the MMTV 3' LTR ORF coding sequence, 10-15% of splenic B cells reacted by immunoperoxidase staining with this reagent, while no such staining was observed in splenic T cell preparations. Furthermore, in preparations of splenic E cells, but not T cells, two bands of 68 and 33 kDa, respectively, were detected by Western blots using the anti-ORF. These results demonstrate that the superantigen protein is present in B cells of BALB/c mice in two distinct forms, i.e., as a 68-kDa molecule, possibly associated with products of the env gene, and as a 33-kDa form.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalCellular Immunology
Volume163
Issue number2
DOIs
StatePublished - Jul 14 1995

Fingerprint

Mouse mammary tumor virus
Superantigens
B-Lymphocytes
Open Reading Frames
Genes
Viral Envelope Proteins
Terminal Repeat Sequences
Western Blotting
T-Lymphocytes
Minor Lymphocyte Stimulatory Antigens
env Gene Products
Staining and Labeling
Oncogenic Viruses
Collodion
Antibodies
Proteins
Breast Neoplasms
Antigens

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Detection of retroviral superantigen and products of the envelope gene from endogenous mouse mammary tumor virus in B cells from BALB/c mice. / Lopez-Cepero, Mayra; Wang, Yang; Keydar, Iafa; Brandt-Carlson, Carolyn; Butel, Janet S.; Lopez, Diana M.

In: Cellular Immunology, Vol. 163, No. 2, 14.07.1995, p. 191-197.

Research output: Contribution to journalArticle

Lopez-Cepero, Mayra ; Wang, Yang ; Keydar, Iafa ; Brandt-Carlson, Carolyn ; Butel, Janet S. ; Lopez, Diana M. / Detection of retroviral superantigen and products of the envelope gene from endogenous mouse mammary tumor virus in B cells from BALB/c mice. In: Cellular Immunology. 1995 ; Vol. 163, No. 2. pp. 191-197.
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