Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus

C. Muller, D. Kagi, T. Aebischer, B. Odermatt, W. Held, E. R. Podack, R. M. Zinkernagel, H. Hengartner

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The analysis of gene expression in cytotoxic T cells by in situ hybridization of serial liver and brain sections from mice infected with lymphocytic choriomeningitis virus (LCMV) and immunostaining with T cell marker- and virus-specific antibodies revealed a close histological association of infiltrating lymphocytes expressing the perforin and granzyme A genes with virally infected cells. Maximal frequency of perforin and granzyme A mRNA-containing cells on liver sections preceded by about 2 days maximal LCMV-specific cytotoxicity of the lymphoid liver infiltrating cells. These results are most consistent with an involvement of perforin and granzyme A in cell-mediated cytotoxicity in vivo.

Original languageEnglish
Pages (from-to)1253-1259
Number of pages7
JournalEuropean Journal of Immunology
Volume19
Issue number7
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Lymphocytic choriomeningitis virus
Granzymes
Perforin
Messenger RNA
Infection
Liver
vif Genes
T-Lymphocytes
In Situ Hybridization
Lymphocytes
Viruses
Gene Expression
Antibodies
Brain

ASJC Scopus subject areas

  • Immunology

Cite this

Muller, C., Kagi, D., Aebischer, T., Odermatt, B., Held, W., Podack, E. R., ... Hengartner, H. (1989). Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus. European Journal of Immunology, 19(7), 1253-1259.

Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus. / Muller, C.; Kagi, D.; Aebischer, T.; Odermatt, B.; Held, W.; Podack, E. R.; Zinkernagel, R. M.; Hengartner, H.

In: European Journal of Immunology, Vol. 19, No. 7, 01.01.1989, p. 1253-1259.

Research output: Contribution to journalArticle

Muller, C, Kagi, D, Aebischer, T, Odermatt, B, Held, W, Podack, ER, Zinkernagel, RM & Hengartner, H 1989, 'Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus', European Journal of Immunology, vol. 19, no. 7, pp. 1253-1259.
Muller, C. ; Kagi, D. ; Aebischer, T. ; Odermatt, B. ; Held, W. ; Podack, E. R. ; Zinkernagel, R. M. ; Hengartner, H. / Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus. In: European Journal of Immunology. 1989 ; Vol. 19, No. 7. pp. 1253-1259.
@article{711df62204ed4cf0ae7129a75dde6136,
title = "Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus",
abstract = "The analysis of gene expression in cytotoxic T cells by in situ hybridization of serial liver and brain sections from mice infected with lymphocytic choriomeningitis virus (LCMV) and immunostaining with T cell marker- and virus-specific antibodies revealed a close histological association of infiltrating lymphocytes expressing the perforin and granzyme A genes with virally infected cells. Maximal frequency of perforin and granzyme A mRNA-containing cells on liver sections preceded by about 2 days maximal LCMV-specific cytotoxicity of the lymphoid liver infiltrating cells. These results are most consistent with an involvement of perforin and granzyme A in cell-mediated cytotoxicity in vivo.",
author = "C. Muller and D. Kagi and T. Aebischer and B. Odermatt and W. Held and Podack, {E. R.} and Zinkernagel, {R. M.} and H. Hengartner",
year = "1989",
month = "1",
day = "1",
language = "English",
volume = "19",
pages = "1253--1259",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "7",

}

TY - JOUR

T1 - Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus

AU - Muller, C.

AU - Kagi, D.

AU - Aebischer, T.

AU - Odermatt, B.

AU - Held, W.

AU - Podack, E. R.

AU - Zinkernagel, R. M.

AU - Hengartner, H.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - The analysis of gene expression in cytotoxic T cells by in situ hybridization of serial liver and brain sections from mice infected with lymphocytic choriomeningitis virus (LCMV) and immunostaining with T cell marker- and virus-specific antibodies revealed a close histological association of infiltrating lymphocytes expressing the perforin and granzyme A genes with virally infected cells. Maximal frequency of perforin and granzyme A mRNA-containing cells on liver sections preceded by about 2 days maximal LCMV-specific cytotoxicity of the lymphoid liver infiltrating cells. These results are most consistent with an involvement of perforin and granzyme A in cell-mediated cytotoxicity in vivo.

AB - The analysis of gene expression in cytotoxic T cells by in situ hybridization of serial liver and brain sections from mice infected with lymphocytic choriomeningitis virus (LCMV) and immunostaining with T cell marker- and virus-specific antibodies revealed a close histological association of infiltrating lymphocytes expressing the perforin and granzyme A genes with virally infected cells. Maximal frequency of perforin and granzyme A mRNA-containing cells on liver sections preceded by about 2 days maximal LCMV-specific cytotoxicity of the lymphoid liver infiltrating cells. These results are most consistent with an involvement of perforin and granzyme A in cell-mediated cytotoxicity in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0024379645&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024379645&partnerID=8YFLogxK

M3 - Article

VL - 19

SP - 1253

EP - 1259

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 7

ER -