Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus

Christoph Müller; David Kägi; Toni Aebischer; Bernhard Odermatt; Werner Held; Eckhard R. Podack; Rolf M. Zinkernagel; Hans Hengartner

doi:10.1002/eji.1830190716

Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus

Christoph Müller, David Kägi, Toni Aebischer, Bernhard Odermatt, Werner Held, Eckhard R. Podack, Rolf M. Zinkernagel, Hans Hengartner

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

The analysis of gene expression in cytotoxic T cells by in situ hybridization of serial liver and brain sections from mice infected with lymphocytic choriomeningitis virus (LCMV) and immunostaining with T cell marker- and virus-specific antibodies revealed a close histological association of infiltrating lymphocytes expressing the perforin and granzyme A genes with virally infected cells. Maximal frequency of perforin and granzyme A mRNA-containing cells on liver sections preceded by about 2 days maximal LCMV-specific cytotoxicity of the lymphoid liver infiltrating cells. These results are most consistent with an involvement of perforin and granzyme A in cell-mediated cytotoxicity in vivo.

Original language	English (US)
Pages (from-to)	1253-1259
Number of pages	7
Journal	European Journal of Immunology
Volume	19
Issue number	7
DOIs	https://doi.org/10.1002/eji.1830190716
State	Published - Jul 1989

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus. / Müller, Christoph; Kägi, David; Aebischer, Toni; Odermatt, Bernhard; Held, Werner; Podack, Eckhard R.; Zinkernagel, Rolf M.; Hengartner, Hans.

In: European Journal of Immunology, Vol. 19, No. 7, 07.1989, p. 1253-1259.

Research output: Contribution to journal › Article › peer-review

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abstract = "The analysis of gene expression in cytotoxic T cells by in situ hybridization of serial liver and brain sections from mice infected with lymphocytic choriomeningitis virus (LCMV) and immunostaining with T cell marker- and virus-specific antibodies revealed a close histological association of infiltrating lymphocytes expressing the perforin and granzyme A genes with virally infected cells. Maximal frequency of perforin and granzyme A mRNA-containing cells on liver sections preceded by about 2 days maximal LCMV-specific cytotoxicity of the lymphoid liver infiltrating cells. These results are most consistent with an involvement of perforin and granzyme A in cell-mediated cytotoxicity in vivo.",

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AU - Held, Werner

AU - Podack, Eckhard R.

AU - Zinkernagel, Rolf M.

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