Detection of perforin and granzyme A mRNA in infiltrating cells during infection of mice with lymphocytic choriomeningitis virus

Christoph Müller, David Kägi, Toni Aebischer, Bernhard Odermatt, Werner Held, Eckhard R. Podack, Rolf M. Zinkernagel, Hans Hengartner

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

The analysis of gene expression in cytotoxic T cells by in situ hybridization of serial liver and brain sections from mice infected with lymphocytic choriomeningitis virus (LCMV) and immunostaining with T cell marker- and virus-specific antibodies revealed a close histological association of infiltrating lymphocytes expressing the perforin and granzyme A genes with virally infected cells. Maximal frequency of perforin and granzyme A mRNA-containing cells on liver sections preceded by about 2 days maximal LCMV-specific cytotoxicity of the lymphoid liver infiltrating cells. These results are most consistent with an involvement of perforin and granzyme A in cell-mediated cytotoxicity in vivo.

Original languageEnglish (US)
Pages (from-to)1253-1259
Number of pages7
JournalEuropean Journal of Immunology
Volume19
Issue number7
DOIs
StatePublished - Jul 1989

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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