Detection of p53 gene abnormality by sequence analysis of archival paraffin tissue

A comparison with fresh-frozen specimens

Mehrdad Nadji, L. Meng, L. Lin, M. Nassiri, Azorides R Morales

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This parallel study was designed to compare the sensitivity and specificity of detection of point mutations in fresh-frozen and formalin- fixed, paraffin-embedded breast cancer tissue. Sequence analysis of exon 5 of p53 gene was performed on polymerase chain reaction-amplified DNA from 25 infiltrating ductal carcinomas of the breast. Four tumors showed mutations with identical base substitutions in their respective codons of both frozen and paraffin-embedded specimens. We conclude that subtle genetic alterations can be detected in archival paraffin tissue with an accuracy comparable to that of fresh-frozen histologic samples.

Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalDiagnostic Molecular Pathology
Volume5
Issue number4
DOIs
StatePublished - Dec 23 1996

Fingerprint

p53 Genes
Paraffin
Sequence Analysis
Carcinoma, Ductal, Breast
Point Mutation
Codon
Formaldehyde
Exons
Breast Neoplasms
Sensitivity and Specificity
Polymerase Chain Reaction
Mutation
DNA
Neoplasms

Keywords

  • Breast cancer
  • DNA sequencing
  • p53
  • Paraffin-embedded tissue

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Detection of p53 gene abnormality by sequence analysis of archival paraffin tissue : A comparison with fresh-frozen specimens. / Nadji, Mehrdad; Meng, L.; Lin, L.; Nassiri, M.; Morales, Azorides R.

In: Diagnostic Molecular Pathology, Vol. 5, No. 4, 23.12.1996, p. 279-283.

Research output: Contribution to journalArticle

@article{03b2dce27644420489f2001f95b72877,
title = "Detection of p53 gene abnormality by sequence analysis of archival paraffin tissue: A comparison with fresh-frozen specimens",
abstract = "This parallel study was designed to compare the sensitivity and specificity of detection of point mutations in fresh-frozen and formalin- fixed, paraffin-embedded breast cancer tissue. Sequence analysis of exon 5 of p53 gene was performed on polymerase chain reaction-amplified DNA from 25 infiltrating ductal carcinomas of the breast. Four tumors showed mutations with identical base substitutions in their respective codons of both frozen and paraffin-embedded specimens. We conclude that subtle genetic alterations can be detected in archival paraffin tissue with an accuracy comparable to that of fresh-frozen histologic samples.",
keywords = "Breast cancer, DNA sequencing, p53, Paraffin-embedded tissue",
author = "Mehrdad Nadji and L. Meng and L. Lin and M. Nassiri and Morales, {Azorides R}",
year = "1996",
month = "12",
day = "23",
doi = "10.1097/00019606-199612000-00009",
language = "English",
volume = "5",
pages = "279--283",
journal = "Diagnostic Molecular Pathology",
issn = "1052-9551",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Detection of p53 gene abnormality by sequence analysis of archival paraffin tissue

T2 - A comparison with fresh-frozen specimens

AU - Nadji, Mehrdad

AU - Meng, L.

AU - Lin, L.

AU - Nassiri, M.

AU - Morales, Azorides R

PY - 1996/12/23

Y1 - 1996/12/23

N2 - This parallel study was designed to compare the sensitivity and specificity of detection of point mutations in fresh-frozen and formalin- fixed, paraffin-embedded breast cancer tissue. Sequence analysis of exon 5 of p53 gene was performed on polymerase chain reaction-amplified DNA from 25 infiltrating ductal carcinomas of the breast. Four tumors showed mutations with identical base substitutions in their respective codons of both frozen and paraffin-embedded specimens. We conclude that subtle genetic alterations can be detected in archival paraffin tissue with an accuracy comparable to that of fresh-frozen histologic samples.

AB - This parallel study was designed to compare the sensitivity and specificity of detection of point mutations in fresh-frozen and formalin- fixed, paraffin-embedded breast cancer tissue. Sequence analysis of exon 5 of p53 gene was performed on polymerase chain reaction-amplified DNA from 25 infiltrating ductal carcinomas of the breast. Four tumors showed mutations with identical base substitutions in their respective codons of both frozen and paraffin-embedded specimens. We conclude that subtle genetic alterations can be detected in archival paraffin tissue with an accuracy comparable to that of fresh-frozen histologic samples.

KW - Breast cancer

KW - DNA sequencing

KW - p53

KW - Paraffin-embedded tissue

UR - http://www.scopus.com/inward/record.url?scp=0029952902&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029952902&partnerID=8YFLogxK

U2 - 10.1097/00019606-199612000-00009

DO - 10.1097/00019606-199612000-00009

M3 - Article

VL - 5

SP - 279

EP - 283

JO - Diagnostic Molecular Pathology

JF - Diagnostic Molecular Pathology

SN - 1052-9551

IS - 4

ER -