Detection of p53 gene abnormality by sequence analysis of archival paraffin tissue: A comparison with fresh-frozen specimens

M. Nadji, L. Meng, L. Lin, M. Nassiri, A. R. Morales

Research output: Contribution to journalArticle

15 Scopus citations


This parallel study was designed to compare the sensitivity and specificity of detection of point mutations in fresh-frozen and formalin- fixed, paraffin-embedded breast cancer tissue. Sequence analysis of exon 5 of p53 gene was performed on polymerase chain reaction-amplified DNA from 25 infiltrating ductal carcinomas of the breast. Four tumors showed mutations with identical base substitutions in their respective codons of both frozen and paraffin-embedded specimens. We conclude that subtle genetic alterations can be detected in archival paraffin tissue with an accuracy comparable to that of fresh-frozen histologic samples.

Original languageEnglish (US)
Pages (from-to)279-283
Number of pages5
JournalDiagnostic Molecular Pathology
Issue number4
StatePublished - Dec 23 1996



  • Breast cancer
  • DNA sequencing
  • p53
  • Paraffin-embedded tissue

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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