Detection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase

Jhony Orbulescu; Celeste A. Constantine; Vipin K. Rastogi; Saumil S. Shah; Joseph J. DeFrank; Roger M. Leblanc

doi:10.1021/ac061118m

Detection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase

Jhony Orbulescu, Celeste A. Constantine, Vipin K. Rastogi, Saumil S. Shah, Joseph J. DeFrank, Roger M. Leblanc

Chemistry

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection. The substrate was cleaned and modified prior to chemical attachment. Each modification step was monitored by imaging ellipsometry as the thickness increased with each modification step. The chemically attached OPH was labeled with a fluorescent dye (7-isothiocyanato-4-methylcoumarin) for the detection of paraoxon in aqueous solution, ranging from 10^-9 to 10^-5 M. UV-visible spectra were also acquired for the determination of the hydrolysis product of paraoxon, namely p-nitrophenol.

Original language	English (US)
Pages (from-to)	7016-7021
Number of pages	6
Journal	Analytical Chemistry
Volume	78
Issue number	19
DOIs	https://doi.org/10.1021/ac061118m
State	Published - Oct 1 2006

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac061118m

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abstract = "As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection. The substrate was cleaned and modified prior to chemical attachment. Each modification step was monitored by imaging ellipsometry as the thickness increased with each modification step. The chemically attached OPH was labeled with a fluorescent dye (7-isothiocyanato-4-methylcoumarin) for the detection of paraoxon in aqueous solution, ranging from 10-9 to 10-5 M. UV-visible spectra were also acquired for the determination of the hydrolysis product of paraoxon, namely p-nitrophenol.",

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AU - Orbulescu, Jhony

AU - Constantine, Celeste A.

AU - Rastogi, Vipin K.

AU - Shah, Saumil S.

AU - DeFrank, Joseph J.

AU - Leblanc, Roger M.

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