Detection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase

Jhony Orbulescu, Celeste A. Constantine, Vipin K. Rastogi, Saumil S. Shah, Joseph J. DeFrank, Roger Leblanc

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection. The substrate was cleaned and modified prior to chemical attachment. Each modification step was monitored by imaging ellipsometry as the thickness increased with each modification step. The chemically attached OPH was labeled with a fluorescent dye (7-isothiocyanato-4-methylcoumarin) for the detection of paraoxon in aqueous solution, ranging from 10-9 to 10-5 M. UV-visible spectra were also acquired for the determination of the hydrolysis product of paraoxon, namely p-nitrophenol.

Original languageEnglish
Pages (from-to)7016-7021
Number of pages6
JournalAnalytical Chemistry
Volume78
Issue number19
DOIs
StatePublished - Oct 1 2006

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Organophosphorus Compounds
Paraoxon
Aryldialkylphosphatase
Quartz
Ellipsometry
Substrates
Fluorescent Dyes
Hydrolysis
Imaging techniques
Enzymes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Orbulescu, J., Constantine, C. A., Rastogi, V. K., Shah, S. S., DeFrank, J. J., & Leblanc, R. (2006). Detection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase. Analytical Chemistry, 78(19), 7016-7021. https://doi.org/10.1021/ac061118m

Detection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase. / Orbulescu, Jhony; Constantine, Celeste A.; Rastogi, Vipin K.; Shah, Saumil S.; DeFrank, Joseph J.; Leblanc, Roger.

In: Analytical Chemistry, Vol. 78, No. 19, 01.10.2006, p. 7016-7021.

Research output: Contribution to journalArticle

Orbulescu, J, Constantine, CA, Rastogi, VK, Shah, SS, DeFrank, JJ & Leblanc, R 2006, 'Detection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase', Analytical Chemistry, vol. 78, no. 19, pp. 7016-7021. https://doi.org/10.1021/ac061118m
Orbulescu, Jhony ; Constantine, Celeste A. ; Rastogi, Vipin K. ; Shah, Saumil S. ; DeFrank, Joseph J. ; Leblanc, Roger. / Detection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase. In: Analytical Chemistry. 2006 ; Vol. 78, No. 19. pp. 7016-7021.
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