Detection of human papillomavirus DNA in invasive carcinomas of the cervix by in situ hybridization

R. S. Ostrow, D. A. Manias, B. A. Clark, T. Okagaki, L. B. Twiggs, A. J. Faras

Research output: Contribution to journalArticle

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Abstract

An examination of 27 invasive cancers of the cervix was performed using the technique of in situ hybridization using human papillomavirus DNA probes. Four tissues, previously found to harbor papillomavirus DNA by filter hybridization, were confirmed by in situ analysis. One further tissue never previously studied was also found to be positive by in situ hybridization. Overall, we found 33% of invasive cancers of the cervix to contain human papillomavirus DNA. In contrast, 55% of carcinoma in situ and severe dysplasia of the cervix were found to be positive for human papillomavirus DNA. These results confirmed that the sample population of patients in our studies have a relatively low association of human papillomavirus DNA with invasive cancers of the cervix and that in situ hybridization provides an effective complementation to filter hybridization for human papillomavirus-infected tumors.

Original languageEnglish
Pages (from-to)649-653
Number of pages5
JournalCancer Research
Volume47
Issue number2
StatePublished - Apr 2 1987
Externally publishedYes

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Carcinoma in Situ
Cervix Uteri
In Situ Hybridization
Uterine Cervical Neoplasms
DNA
Uterine Cervical Dysplasia
DNA Probes
Population
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ostrow, R. S., Manias, D. A., Clark, B. A., Okagaki, T., Twiggs, L. B., & Faras, A. J. (1987). Detection of human papillomavirus DNA in invasive carcinomas of the cervix by in situ hybridization. Cancer Research, 47(2), 649-653.

Detection of human papillomavirus DNA in invasive carcinomas of the cervix by in situ hybridization. / Ostrow, R. S.; Manias, D. A.; Clark, B. A.; Okagaki, T.; Twiggs, L. B.; Faras, A. J.

In: Cancer Research, Vol. 47, No. 2, 02.04.1987, p. 649-653.

Research output: Contribution to journalArticle

Ostrow, RS, Manias, DA, Clark, BA, Okagaki, T, Twiggs, LB & Faras, AJ 1987, 'Detection of human papillomavirus DNA in invasive carcinomas of the cervix by in situ hybridization', Cancer Research, vol. 47, no. 2, pp. 649-653.
Ostrow RS, Manias DA, Clark BA, Okagaki T, Twiggs LB, Faras AJ. Detection of human papillomavirus DNA in invasive carcinomas of the cervix by in situ hybridization. Cancer Research. 1987 Apr 2;47(2):649-653.
Ostrow, R. S. ; Manias, D. A. ; Clark, B. A. ; Okagaki, T. ; Twiggs, L. B. ; Faras, A. J. / Detection of human papillomavirus DNA in invasive carcinomas of the cervix by in situ hybridization. In: Cancer Research. 1987 ; Vol. 47, No. 2. pp. 649-653.
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