Detection of human antibodies against Plasmodium falciparum antigens in blood meals of anopheline mosquitoes.

C. E. Contreras, John C Beier

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1 Citation (Scopus)

Abstract

Human IgG antibodies against Plasmodium falciparum asexual stages, gametocytes and sporozoites were detected by indirect fluorescent antibody (IFA) techniques in the blood meals of Anopheles gambiae s.l. from a malaria-endemic area of western Kenya. Field-collected mosquitoes, which had been stored dry for over 2 years, were screened first for human IgG by ELISA. In 141 blood meal samples from human-fed mosquitoes, the prevalence of stage-specific antibodies was 87.9% for asexual-stage parasites, 78.0% for gametocytes, and 87.9% for sporozoites. There were no differences in the prevalence of stage-specific antibodies for mosquitoes collected from 2 sites, before and after the long rainy season of 1988. The detection of specific human antibodies in mosquito blood meals by IFA, or by more efficient methods, may provide alternative approaches for large-scale, epidemiologic studies of malaria and other vector-borne diseases.

Original languageEnglish
Pages (from-to)252-255
Number of pages4
JournalJournal of the American Mosquito Control Association
Volume8
Issue number3
StatePublished - Sep 1 1992
Externally publishedYes

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blood meal
Plasmodium falciparum
antigen
Culicidae
mosquito
antibody
Meals
blood
antigens
Antigens
antibodies
Antibodies
Sporozoites
gametocytes
sporozoites
malaria
Malaria
Immunoglobulin G
Anopheles gambiae
Disease Vectors

ASJC Scopus subject areas

  • Insect Science

Cite this

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abstract = "Human IgG antibodies against Plasmodium falciparum asexual stages, gametocytes and sporozoites were detected by indirect fluorescent antibody (IFA) techniques in the blood meals of Anopheles gambiae s.l. from a malaria-endemic area of western Kenya. Field-collected mosquitoes, which had been stored dry for over 2 years, were screened first for human IgG by ELISA. In 141 blood meal samples from human-fed mosquitoes, the prevalence of stage-specific antibodies was 87.9{\%} for asexual-stage parasites, 78.0{\%} for gametocytes, and 87.9{\%} for sporozoites. There were no differences in the prevalence of stage-specific antibodies for mosquitoes collected from 2 sites, before and after the long rainy season of 1988. The detection of specific human antibodies in mosquito blood meals by IFA, or by more efficient methods, may provide alternative approaches for large-scale, epidemiologic studies of malaria and other vector-borne diseases.",
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