Detection of human acid α-glucosidase in fibroblasts using monoclonal antibodies in a biotin-avidin amplified ELISA

R. D. Henkel, G. R. Dreesman, R. C. Kennedy, R. R. Howell, J. C. Williams

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A sensitive and specific immunological assay for detection of human lysosomal α-glucosidase was developed using a mouse monoclonal antibody incorporated into a biotin-avidin amplified ELISA. The immunoassay was more than 60 times more sensitive than the currently used enzymatic assay for α-glucosidase activity using a fluorimetric substrate. This methodology provides an alternative approach with increased sensitivity for screening individuals for α-glucosidase deficiency.

Original languageEnglish (US)
Pages (from-to)351-360
Number of pages10
JournalHybridoma
Volume4
Issue number4
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Genetics

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