Detection of HIV-1 gene sequences in hippocampal neurons isolated from postmortem AIDS brains by laser capture microdissection

Jorge Torres-Muñoz, Patricia Stockton, Noe Tacoronte, Brenda Roberts, Robert R. Maronpot, Carol Petito

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

We employed laser capture microdissection to remove individual pyramidal neurons from the CA1, CA3, and CA4 regions of formalin-fixed, paraffin-embedded hippocampus from 8 AIDS brains and 2 HIV-1-seronegative normal brains. We amplified HIV-1 gag and nef gene sequences using separate, double round PCR reactions for each of the primer sets. In all 3 hippocampal regions, amplification efficiency was best with sequence length between 284 and 324 bp; HIV-1 nef gene sequences were more common than HIV-1 gag sequences; and rank order for percent positive amplification was CA3 > CA4 > CA1 samples. These results are the first to detect HIV-1 gene sequences in microdissected human tissue. They indicate that brain neurons in vivo contain HIV-1 DNA sequences consistent with latent infection by this virus, and suggest that neurons display a selective vulnerability for HIV infection. Neuronal HIV infection could contribute to neuronal injury and death or act as a potential viral reservoir if reactivated.

Original languageEnglish
Pages (from-to)885-892
Number of pages8
JournalJournal of Neuropathology and Experimental Neurology
Volume60
Issue number9
StatePublished - Oct 1 2001

Fingerprint

Laser Capture Microdissection
HIV-1
Acquired Immunodeficiency Syndrome
Neurons
Brain
nef Genes
Genes
HIV Infections
gag Genes
Pyramidal Cells
Virus Diseases
Paraffin
Formaldehyde
Hippocampus
Polymerase Chain Reaction
Wounds and Injuries

Keywords

  • Hippocampus
  • HIV
  • HIV encephalitis
  • HIV-associated dementia
  • Laser capture microdissection
  • Polymerase chain reaction (PCR)

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Torres-Muñoz, J., Stockton, P., Tacoronte, N., Roberts, B., Maronpot, R. R., & Petito, C. (2001). Detection of HIV-1 gene sequences in hippocampal neurons isolated from postmortem AIDS brains by laser capture microdissection. Journal of Neuropathology and Experimental Neurology, 60(9), 885-892.

Detection of HIV-1 gene sequences in hippocampal neurons isolated from postmortem AIDS brains by laser capture microdissection. / Torres-Muñoz, Jorge; Stockton, Patricia; Tacoronte, Noe; Roberts, Brenda; Maronpot, Robert R.; Petito, Carol.

In: Journal of Neuropathology and Experimental Neurology, Vol. 60, No. 9, 01.10.2001, p. 885-892.

Research output: Contribution to journalArticle

Torres-Muñoz, J, Stockton, P, Tacoronte, N, Roberts, B, Maronpot, RR & Petito, C 2001, 'Detection of HIV-1 gene sequences in hippocampal neurons isolated from postmortem AIDS brains by laser capture microdissection', Journal of Neuropathology and Experimental Neurology, vol. 60, no. 9, pp. 885-892.
Torres-Muñoz, Jorge ; Stockton, Patricia ; Tacoronte, Noe ; Roberts, Brenda ; Maronpot, Robert R. ; Petito, Carol. / Detection of HIV-1 gene sequences in hippocampal neurons isolated from postmortem AIDS brains by laser capture microdissection. In: Journal of Neuropathology and Experimental Neurology. 2001 ; Vol. 60, No. 9. pp. 885-892.
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