Detection of anti-hepatitis C virus antibodies in patients undergoing dialysis by utilizing a hepatitis C virus 3,0 assay: Correlation with hepatitis C virus RNA

M. De Medina, M. Hill, H. O. Sullivan, B. Leclerq, J. P. Pennell, L. Jeffers, K. R. Reddy, E. R. Schiff, G. O. Perez

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Hepatitis C virus (HCV) infection is endemic in long-term dialysis units. We assessed the performance of a recently developed HCV 3.0 assay for the detection of HCV antibodies in patients undergoing dialysis. The study evaluated 128 patients undergoing long-term maintenance hemodialysis. Anti-HCV was detected by 2.0 and 3.0 enzyme immunoassay (EIA). Results were confirmed with recombinant immunoblot assays (RIBA(TM) 2.0 and RIBA(TM) 3.0). HCV RNA was detected by using reverse transcriptase-polymerase chain reaction (RT-PCR). Thirty-two patients (25%) were HCV EIA 2.0 positive. Of these, 1 was RIBA(TM) 2.0 negative (PCR positive), 3 were indeterminate (3 PCR positive), and 28 were positive (23 PCR positive). Thirty-five (27%) were HCV EIA 3.0 positive. One was RIBA(TM) 3.0 negative (PCR positive), 1 was indeterminate (c33c, PCR positive), and 33 were positive (27 PCR positive) by RIBA(TM) 3.0. Thus only 1 PCR-positive patient was negative with RIBA(TM) 2.0 and 3.0 assays. Two of the 3 RIBA(TM) 2.0 indeterminate samples were positive with RIBA(TM) 3.0. One remained indeterminate but was HCV RNA positive. In summary, HCV 3.0 EIA detected 4 additional viremic patients but was positive in 6 PCR-negative subjects. A high correlation of the presence of antibody to c33c with HCV RNA (28 of 34, 82%) was found, and it was found in all anti-HCV positive samples and in 1 indeterminate sample. We conclude that the HCV EIA 3.0 test with the supplemental confirmatory RIBA(TM) 3.0 test may improve the sensitivity for the detection of anti-HCV. Nevertheless, in potentially immunocompromised patients undergoing dialysis, PCR continues to be the only reliable test for detecting viremia.

Original languageEnglish (US)
Pages (from-to)73-75
Number of pages3
JournalJournal of Laboratory and Clinical Medicine
Volume132
Issue number1
DOIs
StatePublished - Jul 1998

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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