Detection of an altered heterochromatin structure in the absence of the nucleotide excision repair protein Rad4 in Saccharomtyces cerevisiae

Ling Zhang, Hua Chen, Xin Bi, Feng Gong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Rad4p is a DNA damage recognition protein essential for global genomic nucleotide excision repair in Saccharomyces cerevisiae. Here, we show that Rad4p binds to the heterochromatic HML locus. In a yeast mutant lacking Rad4p, an increased level of SIR complex binding at the HML locus is accompanied by an altered, more compact heterochromatin structure, as revealed by a topological analysis of chromatin circles released from the locus. In addition, gene silencing at the HML locus is enhanced in the rad4Δ mutant. Importantly, re-expression of Rad4p in the rad4Δ mutant restores the altered heterochromatin structure to a conformation similar to that detected in wild-type cells. These findings reveal a novel role of Rad4p in the regulation of heterochromatin structure and gene silencing.

Original languageEnglish
Pages (from-to)2435-2442
Number of pages8
JournalCell Cycle
Volume12
Issue number15
DOIs
StatePublished - Aug 1 2013

Fingerprint

Heterochromatin
DNA Repair
Gene Silencing
Proteins
DNA Damage
Chromatin
Saccharomyces cerevisiae
Yeasts

Keywords

  • Heterochromatin
  • HML
  • Nucleotide excision repair
  • Rad4p
  • SIR complex

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

Detection of an altered heterochromatin structure in the absence of the nucleotide excision repair protein Rad4 in Saccharomtyces cerevisiae. / Zhang, Ling; Chen, Hua; Bi, Xin; Gong, Feng.

In: Cell Cycle, Vol. 12, No. 15, 01.08.2013, p. 2435-2442.

Research output: Contribution to journalArticle

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