Abstract
We developed antigen-nonspecific enzyme-linked immunoassays (ELISA) to quantitate IgG-C3- and IgM-C3-containing circulating immune complexes (CIC) in venous and arterial blood from rheumatic disease patients. Standards were diethylaminoethyl (DEAE)-purified, heat-aggregated IgG incubated with fresh human serum (for IgG-C3 CIC) and IgM rheumatoid factor-rich serum incubated with reduced, alkylated IgG and then with fresh human serum (for IgM-IgG-C3 CIC). Venous serum and plasma IgG-C3 and IgM-C3 CIC correlated closely (P < 0.01). Rheumatoid arthritis (RA) and systemic lupus erythematous (SLE) patients had elevated levels of venous IgM-C3 CIC (P < 0.0001) but not IgG-C3 aIC; patients with vasculitis, inflammatory rheumatic diseases, or noninflammatory rheumatic diseases had mean values similar to normal individuals. Venous IgG-C3 and IgM-C3 CIC did not correlate. Paired venous and arterial samples from 16 rheumatic disease patients averaged comparable amounts of IgG-C3 and IgM-C3 CIC, respectively; venous and arterial IgM-C3 CIC levels in patients significantly exceeded normals (P < 0.01) Venous and arterial IgG-C3 CIC levels correlated closely (P < 0.01) as did venous dnd arterial IgM-C3 levels (P < 0.05). Thus, arterial CIC offered no advantage over venous determinations for rheumatic disease patients. IgM-C3 CIC were elevated in patients with RA and SLE when IgG-C3 CIC were not. Ig isotype-specific CIC quantitation may be useful for certain rheumatic diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 217-226 |
| Number of pages | 10 |
| Journal | Clinical Immunology and Immunopathology |
| Volume | 36 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Pathology and Forensic Medicine
Cite this
Detection and quantitation of circulating immune complexes in arterial blood of patients with rheumatic disease. / Panush, Richard S.; Katz, Paul; Longley, Selden; Yonker, Richard A.
In: Clinical Immunology and Immunopathology, Vol. 36, No. 2, 01.01.1985, p. 217-226.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection and quantitation of circulating immune complexes in arterial blood of patients with rheumatic disease
AU - Panush, Richard S.
AU - Katz, Paul
AU - Longley, Selden
AU - Yonker, Richard A.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - We developed antigen-nonspecific enzyme-linked immunoassays (ELISA) to quantitate IgG-C3- and IgM-C3-containing circulating immune complexes (CIC) in venous and arterial blood from rheumatic disease patients. Standards were diethylaminoethyl (DEAE)-purified, heat-aggregated IgG incubated with fresh human serum (for IgG-C3 CIC) and IgM rheumatoid factor-rich serum incubated with reduced, alkylated IgG and then with fresh human serum (for IgM-IgG-C3 CIC). Venous serum and plasma IgG-C3 and IgM-C3 CIC correlated closely (P < 0.01). Rheumatoid arthritis (RA) and systemic lupus erythematous (SLE) patients had elevated levels of venous IgM-C3 CIC (P < 0.0001) but not IgG-C3 aIC; patients with vasculitis, inflammatory rheumatic diseases, or noninflammatory rheumatic diseases had mean values similar to normal individuals. Venous IgG-C3 and IgM-C3 CIC did not correlate. Paired venous and arterial samples from 16 rheumatic disease patients averaged comparable amounts of IgG-C3 and IgM-C3 CIC, respectively; venous and arterial IgM-C3 CIC levels in patients significantly exceeded normals (P < 0.01) Venous and arterial IgG-C3 CIC levels correlated closely (P < 0.01) as did venous dnd arterial IgM-C3 levels (P < 0.05). Thus, arterial CIC offered no advantage over venous determinations for rheumatic disease patients. IgM-C3 CIC were elevated in patients with RA and SLE when IgG-C3 CIC were not. Ig isotype-specific CIC quantitation may be useful for certain rheumatic diseases.
AB - We developed antigen-nonspecific enzyme-linked immunoassays (ELISA) to quantitate IgG-C3- and IgM-C3-containing circulating immune complexes (CIC) in venous and arterial blood from rheumatic disease patients. Standards were diethylaminoethyl (DEAE)-purified, heat-aggregated IgG incubated with fresh human serum (for IgG-C3 CIC) and IgM rheumatoid factor-rich serum incubated with reduced, alkylated IgG and then with fresh human serum (for IgM-IgG-C3 CIC). Venous serum and plasma IgG-C3 and IgM-C3 CIC correlated closely (P < 0.01). Rheumatoid arthritis (RA) and systemic lupus erythematous (SLE) patients had elevated levels of venous IgM-C3 CIC (P < 0.0001) but not IgG-C3 aIC; patients with vasculitis, inflammatory rheumatic diseases, or noninflammatory rheumatic diseases had mean values similar to normal individuals. Venous IgG-C3 and IgM-C3 CIC did not correlate. Paired venous and arterial samples from 16 rheumatic disease patients averaged comparable amounts of IgG-C3 and IgM-C3 CIC, respectively; venous and arterial IgM-C3 CIC levels in patients significantly exceeded normals (P < 0.01) Venous and arterial IgG-C3 CIC levels correlated closely (P < 0.01) as did venous dnd arterial IgM-C3 levels (P < 0.05). Thus, arterial CIC offered no advantage over venous determinations for rheumatic disease patients. IgM-C3 CIC were elevated in patients with RA and SLE when IgG-C3 CIC were not. Ig isotype-specific CIC quantitation may be useful for certain rheumatic diseases.
UR - http://www.scopus.com/inward/record.url?scp=0021874257&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021874257&partnerID=8YFLogxK
U2 - 10.1016/0090-1229(85)90123-0
DO - 10.1016/0090-1229(85)90123-0
M3 - Article
C2 - 3874034
AN - SCOPUS:0021874257
VL - 36
SP - 217
EP - 226
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 2
ER -