Detection and quantification of lipid droplets in differentiated human podocytes

Shamroop Kumar Mallela, Devang Maheshkumar Patel, Gloria Michelle Ducasa, Sandra M Merscher-gomez, Alessia Fornoni, Hassan Al-Ali

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lipid droplets (LDs) are dynamic organelles that regulate the storage and homeostasis of intracellular triglycerides and other neutral lipids. Studies show that the number, morphology, and subcellular localization of LDs are altered in a growing number of diseases. As such, methodologies for imaging and quantifying LDs have become essential tools for detecting changes in cellular lipid metabolism, which could be an important indicator of disease onset or progression. We previously reported on the accumulation of LDs in podocytes of the kidney glomerulus in nephrological diseases of metabolic and non-metabolic origin. Here, we describe a high-content analysis (HCA) method for automated detection and quantification of LDs in differentiated human podocytes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages199-206
Number of pages8
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1996
ISSN (Print)1064-3745

Fingerprint

Podocytes
Kidney Glomerulus
Metabolic Diseases
Lipid Metabolism
Organelles
Triglycerides
Homeostasis
Lipid Droplets
Lipids

Keywords

  • Cell-based assay
  • High content screening
  • Human podocytes
  • Lipid droplets
  • Phenotypic drug discovery

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Mallela, S. K., Patel, D. M., Ducasa, G. M., Merscher-gomez, S. M., Fornoni, A., & Al-Ali, H. (2019). Detection and quantification of lipid droplets in differentiated human podocytes. In Methods in Molecular Biology (pp. 199-206). (Methods in Molecular Biology; Vol. 1996). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9488-5_17

Detection and quantification of lipid droplets in differentiated human podocytes. / Mallela, Shamroop Kumar; Patel, Devang Maheshkumar; Ducasa, Gloria Michelle; Merscher-gomez, Sandra M; Fornoni, Alessia; Al-Ali, Hassan.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 199-206 (Methods in Molecular Biology; Vol. 1996).

Research output: Chapter in Book/Report/Conference proceedingChapter

Mallela, SK, Patel, DM, Ducasa, GM, Merscher-gomez, SM, Fornoni, A & Al-Ali, H 2019, Detection and quantification of lipid droplets in differentiated human podocytes. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1996, Humana Press Inc., pp. 199-206. https://doi.org/10.1007/978-1-4939-9488-5_17
Mallela SK, Patel DM, Ducasa GM, Merscher-gomez SM, Fornoni A, Al-Ali H. Detection and quantification of lipid droplets in differentiated human podocytes. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 199-206. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9488-5_17
Mallela, Shamroop Kumar ; Patel, Devang Maheshkumar ; Ducasa, Gloria Michelle ; Merscher-gomez, Sandra M ; Fornoni, Alessia ; Al-Ali, Hassan. / Detection and quantification of lipid droplets in differentiated human podocytes. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 199-206 (Methods in Molecular Biology).
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