Detection and quantification of lipid droplets in differentiated human podocytes

Shamroop Kumar Mallela, Devang Maheshkumar Patel, Gloria Michelle Ducasa, Sandra Merscher, Alessia Fornoni, Hassan Al-Ali

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Lipid droplets (LDs) are dynamic organelles that regulate the storage and homeostasis of intracellular triglycerides and other neutral lipids. Studies show that the number, morphology, and subcellular localization of LDs are altered in a growing number of diseases. As such, methodologies for imaging and quantifying LDs have become essential tools for detecting changes in cellular lipid metabolism, which could be an important indicator of disease onset or progression. We previously reported on the accumulation of LDs in podocytes of the kidney glomerulus in nephrological diseases of metabolic and non-metabolic origin. Here, we describe a high-content analysis (HCA) method for automated detection and quantification of LDs in differentiated human podocytes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages199-206
Number of pages8
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1996
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell-based assay
  • High content screening
  • Human podocytes
  • Lipid droplets
  • Phenotypic drug discovery

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Mallela, S. K., Patel, D. M., Ducasa, G. M., Merscher, S., Fornoni, A., & Al-Ali, H. (2019). Detection and quantification of lipid droplets in differentiated human podocytes. In Methods in Molecular Biology (pp. 199-206). (Methods in Molecular Biology; Vol. 1996). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9488-5_17