Detecting sugar beet syrups in orange juice by D/H and 18O/16O analysis of sucrose

Landis W. Doner, Henry O. Ajie, Leonel Sternberg, John M. Milburn, Michael J. DeNiro, Kevin B. Hicks

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Sucrose from pure orange juices and beet sugar syrups was isolated by preparative HPLC. A portion was converted to sucrose octanitrate, to remove the readily exchangeable hydroxyl hydrogens. D/H ratios were then determined on the carbon-bound hydrogen atoms by mass spectrometric stable-isotope ratio analysis. The mean D/H ratios in orange sucrose were significantly elevated compared to those in beet sucrose, the δ(D) values being -27 and -143/mil respectively. 18O/16O ratios were determined for underivatized sucrose from both soures. With a single exception, δ(18O) values were higher in orange sucrose than in beet sucrose. From these data, a discriminatory formula has been developed describing a 99.99% confidence ellipse about pure orange juices. The availability of this method to identify orange juices as pure or adulterated with beet sugar will provide a disincentive for the economic adulteration of orange juice.

Original languageEnglish
Pages (from-to)610-612
Number of pages3
JournalJournal of Agricultural and Food Chemistry
Volume35
Issue number4
StatePublished - Dec 1 1987

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Sugar beets
Beta vulgaris
syrups
orange juice
sugar beet
Sucrose
sucrose
beet sugar
beets
hydrogen
Hydrogen
adulterated products
Isotopes
Hydroxyl Radical
stable isotopes
Motivation
Carbon
High Pressure Liquid Chromatography
Economics
Availability

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Doner, L. W., Ajie, H. O., Sternberg, L., Milburn, J. M., DeNiro, M. J., & Hicks, K. B. (1987). Detecting sugar beet syrups in orange juice by D/H and 18O/16O analysis of sucrose. Journal of Agricultural and Food Chemistry, 35(4), 610-612.

Detecting sugar beet syrups in orange juice by D/H and 18O/16O analysis of sucrose. / Doner, Landis W.; Ajie, Henry O.; Sternberg, Leonel; Milburn, John M.; DeNiro, Michael J.; Hicks, Kevin B.

In: Journal of Agricultural and Food Chemistry, Vol. 35, No. 4, 01.12.1987, p. 610-612.

Research output: Contribution to journalArticle

Doner, LW, Ajie, HO, Sternberg, L, Milburn, JM, DeNiro, MJ & Hicks, KB 1987, 'Detecting sugar beet syrups in orange juice by D/H and 18O/16O analysis of sucrose', Journal of Agricultural and Food Chemistry, vol. 35, no. 4, pp. 610-612.
Doner LW, Ajie HO, Sternberg L, Milburn JM, DeNiro MJ, Hicks KB. Detecting sugar beet syrups in orange juice by D/H and 18O/16O analysis of sucrose. Journal of Agricultural and Food Chemistry. 1987 Dec 1;35(4):610-612.
Doner, Landis W. ; Ajie, Henry O. ; Sternberg, Leonel ; Milburn, John M. ; DeNiro, Michael J. ; Hicks, Kevin B. / Detecting sugar beet syrups in orange juice by D/H and 18O/16O analysis of sucrose. In: Journal of Agricultural and Food Chemistry. 1987 ; Vol. 35, No. 4. pp. 610-612.
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